Low LINC02147 expression promotes the malignant progression of oral submucous fibrosis
Abstract Background Key lncRNAs associated with the malignant progression of oral submucous fibrosis (OSF) to oral squamous cell carcinoma (OSCC) were identified. Methods Key lncRNAs with sequential changes from normal oral mucosa (NOM) to OSF to OSCC were identified based on the GEO database. Kapla...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-07-01
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| Series: | BMC Oral Health |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12903-022-02346-4 |
| _version_ | 1828190609251237888 |
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| author | Jun Chen Wenjie Li Binjie Liu Xiaoli Xie |
| author_facet | Jun Chen Wenjie Li Binjie Liu Xiaoli Xie |
| author_sort | Jun Chen |
| collection | DOAJ |
| description | Abstract Background Key lncRNAs associated with the malignant progression of oral submucous fibrosis (OSF) to oral squamous cell carcinoma (OSCC) were identified. Methods Key lncRNAs with sequential changes from normal oral mucosa (NOM) to OSF to OSCC were identified based on the GEO database. Kaplan–Meier analysis was used to screen lncRNAs related to OSCC prognosis. Cox regression analysis was used to validate the independent prognostic value. qPCR was used to confirm the expression of the candidate lncRNAs. Gene set enrichment analysis (GSEA), nucleocytoplasmic separation assay, fluorescence in situ hybridization, RNA knockdown, western blot, and cell viability assay were performed to investigate the biological functions of the candidate lncRNA. A nomogram was constructed to quantitatively predict OSCC prognosis based on TCGA. Results Bioinformatics methods indicated that LINC02147 was sequentially downregulated from NOM to OSF to OSCC, as confirmed by clinical tissues and cells. Meanwhile, low LINC02147 expression, as an independent prognostic factor, predicted a poor prognosis for OSCC. GSEA and in vitro studies suggested that low LINC02147 expression promoted OSF malignant progression by promoting cell proliferation and differentiation. A LINC02147 signature-based nomogram successfully quantified each indicator’s contribution to the overall survival of OSCC. Conclusions Low LINC02147 expression promoted OSF malignant progression and predicted poor OSCC prognosis. |
| first_indexed | 2024-04-12T08:25:23Z |
| format | Article |
| id | doaj.art-80146c32416a4f95aa3c6700ae9c5928 |
| institution | Directory Open Access Journal |
| issn | 1472-6831 |
| language | English |
| last_indexed | 2024-04-12T08:25:23Z |
| publishDate | 2022-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Oral Health |
| spelling | doaj.art-80146c32416a4f95aa3c6700ae9c59282022-12-22T03:40:23ZengBMCBMC Oral Health1472-68312022-07-0122111810.1186/s12903-022-02346-4Low LINC02147 expression promotes the malignant progression of oral submucous fibrosisJun Chen0Wenjie Li1Binjie Liu2Xiaoli Xie3Hunan Key Laboratory of Oral Health Research & Hunan 3D, Printing Engineering Research Center of Oral Care and Hunan Clinical Research Center of Oral Major Diseases and Oral Health and Xiangya Stomatological Hospital and Xiangya School of Stomatology, Central South UniversityHunan Key Laboratory of Oral Health Research & Hunan 3D, Printing Engineering Research Center of Oral Care and Hunan Clinical Research Center of Oral Major Diseases and Oral Health and Xiangya Stomatological Hospital and Xiangya School of Stomatology, Central South UniversityHunan Key Laboratory of Oral Health Research & Hunan 3D, Printing Engineering Research Center of Oral Care and Hunan Clinical Research Center of Oral Major Diseases and Oral Health and Xiangya Stomatological Hospital and Xiangya School of Stomatology, Central South UniversityHunan Key Laboratory of Oral Health Research & Hunan 3D, Printing Engineering Research Center of Oral Care and Hunan Clinical Research Center of Oral Major Diseases and Oral Health and Xiangya Stomatological Hospital and Xiangya School of Stomatology, Central South UniversityAbstract Background Key lncRNAs associated with the malignant progression of oral submucous fibrosis (OSF) to oral squamous cell carcinoma (OSCC) were identified. Methods Key lncRNAs with sequential changes from normal oral mucosa (NOM) to OSF to OSCC were identified based on the GEO database. Kaplan–Meier analysis was used to screen lncRNAs related to OSCC prognosis. Cox regression analysis was used to validate the independent prognostic value. qPCR was used to confirm the expression of the candidate lncRNAs. Gene set enrichment analysis (GSEA), nucleocytoplasmic separation assay, fluorescence in situ hybridization, RNA knockdown, western blot, and cell viability assay were performed to investigate the biological functions of the candidate lncRNA. A nomogram was constructed to quantitatively predict OSCC prognosis based on TCGA. Results Bioinformatics methods indicated that LINC02147 was sequentially downregulated from NOM to OSF to OSCC, as confirmed by clinical tissues and cells. Meanwhile, low LINC02147 expression, as an independent prognostic factor, predicted a poor prognosis for OSCC. GSEA and in vitro studies suggested that low LINC02147 expression promoted OSF malignant progression by promoting cell proliferation and differentiation. A LINC02147 signature-based nomogram successfully quantified each indicator’s contribution to the overall survival of OSCC. Conclusions Low LINC02147 expression promoted OSF malignant progression and predicted poor OSCC prognosis.https://doi.org/10.1186/s12903-022-02346-4Oral submucous fibrosis (OSF)Oral squamous cell carcinoma (OSCC)lncRNABiomarkerPrognosis |
| spellingShingle | Jun Chen Wenjie Li Binjie Liu Xiaoli Xie Low LINC02147 expression promotes the malignant progression of oral submucous fibrosis BMC Oral Health Oral submucous fibrosis (OSF) Oral squamous cell carcinoma (OSCC) lncRNA Biomarker Prognosis |
| title | Low LINC02147 expression promotes the malignant progression of oral submucous fibrosis |
| title_full | Low LINC02147 expression promotes the malignant progression of oral submucous fibrosis |
| title_fullStr | Low LINC02147 expression promotes the malignant progression of oral submucous fibrosis |
| title_full_unstemmed | Low LINC02147 expression promotes the malignant progression of oral submucous fibrosis |
| title_short | Low LINC02147 expression promotes the malignant progression of oral submucous fibrosis |
| title_sort | low linc02147 expression promotes the malignant progression of oral submucous fibrosis |
| topic | Oral submucous fibrosis (OSF) Oral squamous cell carcinoma (OSCC) lncRNA Biomarker Prognosis |
| url | https://doi.org/10.1186/s12903-022-02346-4 |
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