Activation of immune evasion machinery is a part of the process of malignant transformation of human cells
Malignant transformation of human cells is associated with their re-programming which results in uncontrolled proliferation and in the same time biochemical activation of immunosuppressive pathways which form cancer immune evasion machinery. However, there is no conceptual understanding of whether i...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2024-01-01
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| Series: | Translational Oncology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1936523323001912 |
| _version_ | 1797422641398677504 |
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| author | Maryam Abooali Inna M. Yasinska Stephanie Schlichtner Sabrina Ruggiero Steffen M. Berger Dietmar Cholewa Milan Milošević Andreas Bartenstein Elizaveta Fasler-Kan Vadim V. Sumbayev |
| author_facet | Maryam Abooali Inna M. Yasinska Stephanie Schlichtner Sabrina Ruggiero Steffen M. Berger Dietmar Cholewa Milan Milošević Andreas Bartenstein Elizaveta Fasler-Kan Vadim V. Sumbayev |
| author_sort | Maryam Abooali |
| collection | DOAJ |
| description | Malignant transformation of human cells is associated with their re-programming which results in uncontrolled proliferation and in the same time biochemical activation of immunosuppressive pathways which form cancer immune evasion machinery. However, there is no conceptual understanding of whether immune evasion machinery pathways and expression of immune checkpoint proteins form a part of the process of malignant transformation or if they are triggered by T lymphocytes and natural killers (NK) attempting to attack cells which are undergoing or already underwent malignant transformation. To address this fundamental question, we performed experimental malignant transformation of BEAS-2B human bronchial epithelium cells and RC-124 non-malignant human kidney epithelial cells using bracken extracts containing carcinogenic alkaloid called ptaquiloside. This transformation led to a significant upregulation of cell proliferation velocity and in the same time led to a significant upregulation in expression of key immune checkpoint proteins – galectin-9, programmed death ligand 1 (PD-L1), indoleamine 2,3-dioxygenase (IDO1). Their increased expression levels were in line with upregulation of the levels and activities of HIF-1 transcription complex and transforming growth factor beta type 1 (TGF-β)-Smad3 signalling pathway. When co-cultured with T cells, transformed epithelial cells displayed much higher and more efficient immune evasion activity compared to original non-transformed cells. Therefore, this work resolved a very important scientific and clinical question and suggested that cancer immune evasion machinery is activated during malignant transformation of human cells regardless the presence of immune cells in microenvironment. |
| first_indexed | 2024-03-09T07:35:23Z |
| format | Article |
| id | doaj.art-8017fc6b56d84459acc2a497ce18cf29 |
| institution | Directory Open Access Journal |
| issn | 1936-5233 |
| language | English |
| last_indexed | 2024-03-09T07:35:23Z |
| publishDate | 2024-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Translational Oncology |
| spelling | doaj.art-8017fc6b56d84459acc2a497ce18cf292023-12-03T05:40:26ZengElsevierTranslational Oncology1936-52332024-01-0139101805Activation of immune evasion machinery is a part of the process of malignant transformation of human cellsMaryam Abooali0Inna M. Yasinska1Stephanie Schlichtner2Sabrina Ruggiero3Steffen M. Berger4Dietmar Cholewa5Milan Milošević6Andreas Bartenstein7Elizaveta Fasler-Kan8Vadim V. Sumbayev9Medway School of Pharmacy, Universities of Kent and Greenwich, Chatham Maritime, United KingdomMedway School of Pharmacy, Universities of Kent and Greenwich, Chatham Maritime, United KingdomMedway School of Pharmacy, Universities of Kent and Greenwich, Chatham Maritime, United Kingdom; DKFZ-Hector Cancer Institute at the University Medical Center Mannheim, Mannheim, Germany; Division of Personalized Medical Oncology (A420), German Cancer Research Center (DKFZ); German Center for Lung Research (DZL), Heidelberg, Germany; Department of Personalized Oncology, Medical Faculty Mannheim, University Hospital Mannheim, University of Heidelberg, Mannheim, GermanyDepartment of Pediatric Surgery, Children's Hospital, Inselspital Bern, University of Bern and Department of Biomedical Research, University of Bern, Bern, SwitzerlandDepartment of Pediatric Surgery, Children's Hospital, Inselspital Bern, University of Bern and Department of Biomedical Research, University of Bern, Bern, SwitzerlandDepartment of Pediatric Surgery, Children's Hospital, Inselspital Bern, University of Bern and Department of Biomedical Research, University of Bern, Bern, SwitzerlandDepartment of Pediatric Surgery, Children's Hospital, Inselspital Bern, University of Bern and Department of Biomedical Research, University of Bern, Bern, SwitzerlandDepartment of Pediatric Surgery, Children's Hospital, Inselspital Bern, University of Bern and Department of Biomedical Research, University of Bern, Bern, SwitzerlandDepartment of Pediatric Surgery, Children's Hospital, Inselspital Bern, University of Bern and Department of Biomedical Research, University of Bern, Bern, Switzerland; Addresses for correspondenceMedway School of Pharmacy, Universities of Kent and Greenwich, Chatham Maritime, United Kingdom; Addresses for correspondenceMalignant transformation of human cells is associated with their re-programming which results in uncontrolled proliferation and in the same time biochemical activation of immunosuppressive pathways which form cancer immune evasion machinery. However, there is no conceptual understanding of whether immune evasion machinery pathways and expression of immune checkpoint proteins form a part of the process of malignant transformation or if they are triggered by T lymphocytes and natural killers (NK) attempting to attack cells which are undergoing or already underwent malignant transformation. To address this fundamental question, we performed experimental malignant transformation of BEAS-2B human bronchial epithelium cells and RC-124 non-malignant human kidney epithelial cells using bracken extracts containing carcinogenic alkaloid called ptaquiloside. This transformation led to a significant upregulation of cell proliferation velocity and in the same time led to a significant upregulation in expression of key immune checkpoint proteins – galectin-9, programmed death ligand 1 (PD-L1), indoleamine 2,3-dioxygenase (IDO1). Their increased expression levels were in line with upregulation of the levels and activities of HIF-1 transcription complex and transforming growth factor beta type 1 (TGF-β)-Smad3 signalling pathway. When co-cultured with T cells, transformed epithelial cells displayed much higher and more efficient immune evasion activity compared to original non-transformed cells. Therefore, this work resolved a very important scientific and clinical question and suggested that cancer immune evasion machinery is activated during malignant transformation of human cells regardless the presence of immune cells in microenvironment.http://www.sciencedirect.com/science/article/pii/S1936523323001912Malignant transformationCo-inhibitory immune checkpointsT cells |
| spellingShingle | Maryam Abooali Inna M. Yasinska Stephanie Schlichtner Sabrina Ruggiero Steffen M. Berger Dietmar Cholewa Milan Milošević Andreas Bartenstein Elizaveta Fasler-Kan Vadim V. Sumbayev Activation of immune evasion machinery is a part of the process of malignant transformation of human cells Translational Oncology Malignant transformation Co-inhibitory immune checkpoints T cells |
| title | Activation of immune evasion machinery is a part of the process of malignant transformation of human cells |
| title_full | Activation of immune evasion machinery is a part of the process of malignant transformation of human cells |
| title_fullStr | Activation of immune evasion machinery is a part of the process of malignant transformation of human cells |
| title_full_unstemmed | Activation of immune evasion machinery is a part of the process of malignant transformation of human cells |
| title_short | Activation of immune evasion machinery is a part of the process of malignant transformation of human cells |
| title_sort | activation of immune evasion machinery is a part of the process of malignant transformation of human cells |
| topic | Malignant transformation Co-inhibitory immune checkpoints T cells |
| url | http://www.sciencedirect.com/science/article/pii/S1936523323001912 |
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