Design of primer pairs for species-specific diagnosis of Leishmania (Leishmania) infantum chagasi using PCR Construção de pares de primers para a detecção espécie-específica de Leishmania (Leishmania) infantum chagasi por PCR

Design of primer pairs for species-specific diagnosis of Leishmania (Leishmania) infantum chagasi using PCR Construção de pares de primers para a detecção espécie-específica de Leishmania (Leishmania) infantum chagasi por PCR

The objective of this study was to design and evaluate new primers for species-specific detection of L. infantum chagasi using PCR. Two combinations of primer pairs were established with the aim of obtaining specific amplification products from the L. infantum chagasi 18S rRNA gene. The combinations...

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Bibliographic Details
Main Authors:	Osvaldo José da Silveira Neto, Sabrina Castilho Duarte, Hérika Xavier da Costa, Guido Fontgalland Coelho Linhares
Format:	Article
Language:	English
Published:	Colégio Brasileiro de Parasitologia Veterinaria 2012-09-01
Series:	Revista Brasileira de Parasitologia Veterinária
Subjects:	Calazar leishmaniose visceral diagnóstico molecular gene 18S rRNA L. infantum chagasi Kala-azar visceral leishmaniasis molecular diagnosis 18S rRNA gene
Online Access:	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612012000300024

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