Microbial Colorants Production in Stirred-Tank Bioreactor and Their Incorporation in an Alternative Food Packaging Biomaterial
Natural colorants from microbial fermentation have gained significant attention in the market to replace the synthetic ones. <i>Talaromyces</i> spp. produce yellow-orange-red colorants, appearing as a potential microorganism to be used for this purpose. In this work, the production of na...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-11-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/6/4/264 |
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| author | Fernanda de Oliveira Caio de Azevedo Lima André Moreni Lopes Daniela de Araújo Viana Marques Janice Izabel Druzian Adalberto Pessoa Júnior Valéria Carvalho Santos-Ebinuma |
| author_facet | Fernanda de Oliveira Caio de Azevedo Lima André Moreni Lopes Daniela de Araújo Viana Marques Janice Izabel Druzian Adalberto Pessoa Júnior Valéria Carvalho Santos-Ebinuma |
| author_sort | Fernanda de Oliveira |
| collection | DOAJ |
| description | Natural colorants from microbial fermentation have gained significant attention in the market to replace the synthetic ones. <i>Talaromyces</i> spp. produce yellow-orange-red colorants, appearing as a potential microorganism to be used for this purpose. In this work, the production of natural colorants by <i>T. amestolkiae</i> in a stirred-tank bioreactor is studied, followed by its application as additives in bio-based films. The effect of the pH-shift control strategy from 4.5 to 8.0 after 96 h of cultivation is evaluated at 500 rpm, resulting in an improvement of natural colorant production, with this increase being more significant for the orange and red ones, both close to 4-fold. Next, the fermented broth containing the colorants is applied to the preparation of cassava starch-based films in order to incorporate functional activity in biodegradable films for food packaging. The presence of fermented broth did not affect the water activity and total solids of biodegradable films as compared with the standard one. In the end, the films are used to pack butter samples (for 45 days) showing excellent results regarding antioxidant activity. It is demonstrated that the presence of natural colorants is obtained by a biotechnology process, which can provide protection against oxidative action, as well as be a functional food additive in food packing biomaterials. |
| first_indexed | 2024-03-10T15:09:08Z |
| format | Article |
| id | doaj.art-802287a97f184133a17f86a9faca04ef |
| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-10T15:09:08Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-802287a97f184133a17f86a9faca04ef2023-11-20T19:29:01ZengMDPI AGJournal of Fungi2309-608X2020-11-016426410.3390/jof6040264Microbial Colorants Production in Stirred-Tank Bioreactor and Their Incorporation in an Alternative Food Packaging BiomaterialFernanda de Oliveira0Caio de Azevedo Lima1André Moreni Lopes2Daniela de Araújo Viana Marques3Janice Izabel Druzian4Adalberto Pessoa Júnior5Valéria Carvalho Santos-Ebinuma6Department of Engineering Bioprocess and Biotechnology, School of Pharmaceutical Sciences, Universidade Estadual Paulista—UNESP, Araraquara 14800-903, BrazilDepartment of Engineering Bioprocess and Biotechnology, School of Pharmaceutical Sciences, Universidade Estadual Paulista—UNESP, Araraquara 14800-903, BrazilFaculty of Pharmaceutical Sciences, University of Campinas—FCF/UNICAMP, Campinas 13083-859, BrazilLaboratory of Biotechnology Applied to Infectious and Parasitic Diseases, Biological Science Institute, University of Pernambuco-ICB/UPE, Recife 50100-130, BrazilDepartment of Bromatological Analysis, Faculty of Pharmacy, Postgraduate Program in Science of Food, Federal University of Bahia, Salvador 40170-115, BrazilDepartment of Biochemical and Pharmaceutical Technology, University of São Paulo, São Paulo 05508-000, BrazilDepartment of Engineering Bioprocess and Biotechnology, School of Pharmaceutical Sciences, Universidade Estadual Paulista—UNESP, Araraquara 14800-903, BrazilNatural colorants from microbial fermentation have gained significant attention in the market to replace the synthetic ones. <i>Talaromyces</i> spp. produce yellow-orange-red colorants, appearing as a potential microorganism to be used for this purpose. In this work, the production of natural colorants by <i>T. amestolkiae</i> in a stirred-tank bioreactor is studied, followed by its application as additives in bio-based films. The effect of the pH-shift control strategy from 4.5 to 8.0 after 96 h of cultivation is evaluated at 500 rpm, resulting in an improvement of natural colorant production, with this increase being more significant for the orange and red ones, both close to 4-fold. Next, the fermented broth containing the colorants is applied to the preparation of cassava starch-based films in order to incorporate functional activity in biodegradable films for food packaging. The presence of fermented broth did not affect the water activity and total solids of biodegradable films as compared with the standard one. In the end, the films are used to pack butter samples (for 45 days) showing excellent results regarding antioxidant activity. It is demonstrated that the presence of natural colorants is obtained by a biotechnology process, which can provide protection against oxidative action, as well as be a functional food additive in food packing biomaterials.https://www.mdpi.com/2309-608X/6/4/264natural colorantsfilamentous fungistirred-tank bioreactorbiodegradable filmsantioxidantfood package |
| spellingShingle | Fernanda de Oliveira Caio de Azevedo Lima André Moreni Lopes Daniela de Araújo Viana Marques Janice Izabel Druzian Adalberto Pessoa Júnior Valéria Carvalho Santos-Ebinuma Microbial Colorants Production in Stirred-Tank Bioreactor and Their Incorporation in an Alternative Food Packaging Biomaterial Journal of Fungi natural colorants filamentous fungi stirred-tank bioreactor biodegradable films antioxidant food package |
| title | Microbial Colorants Production in Stirred-Tank Bioreactor and Their Incorporation in an Alternative Food Packaging Biomaterial |
| title_full | Microbial Colorants Production in Stirred-Tank Bioreactor and Their Incorporation in an Alternative Food Packaging Biomaterial |
| title_fullStr | Microbial Colorants Production in Stirred-Tank Bioreactor and Their Incorporation in an Alternative Food Packaging Biomaterial |
| title_full_unstemmed | Microbial Colorants Production in Stirred-Tank Bioreactor and Their Incorporation in an Alternative Food Packaging Biomaterial |
| title_short | Microbial Colorants Production in Stirred-Tank Bioreactor and Their Incorporation in an Alternative Food Packaging Biomaterial |
| title_sort | microbial colorants production in stirred tank bioreactor and their incorporation in an alternative food packaging biomaterial |
| topic | natural colorants filamentous fungi stirred-tank bioreactor biodegradable films antioxidant food package |
| url | https://www.mdpi.com/2309-608X/6/4/264 |
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