Synthesis and Biological Properties of Alanine-Grafted Hydroxyapatite Nanoparticles
Hydroxyapatite attracts great attention as hard tissues implant material for bones and teeth. Its application in reconstructive medicine depends on its biocompatibility, which is in a function of composition and surface properties. The insertion of a protein element in the composition of implants ca...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-12-01
|
| Series: | Life |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2075-1729/13/1/116 |
| _version_ | 1827623862251028480 |
|---|---|
| author | Bruna Carolina Dorm Mônica Rosas Costa Iemma Benedito Domingos Neto Rauany Cristina Lopes Francisco Ivana Dinić Nenad Ignjatović Smilja Marković Marina Vuković Srečo Škapin Eliane Trovatti Lidija Mančić |
| author_facet | Bruna Carolina Dorm Mônica Rosas Costa Iemma Benedito Domingos Neto Rauany Cristina Lopes Francisco Ivana Dinić Nenad Ignjatović Smilja Marković Marina Vuković Srečo Škapin Eliane Trovatti Lidija Mančić |
| author_sort | Bruna Carolina Dorm |
| collection | DOAJ |
| description | Hydroxyapatite attracts great attention as hard tissues implant material for bones and teeth. Its application in reconstructive medicine depends on its biocompatibility, which is in a function of composition and surface properties. The insertion of a protein element in the composition of implants can improve the cell adhesion and the osseointegration. Having this in mind, the proposal of this work was to develop L-alanine-grafted hydroxyapatite nanoparticles and to study their biocompatibility. Two L-alanine sources and three grafting methods were used for hydroxyapatite surface functionalization. The efficiency of grafting was determined based on X-ray powder diffraction, Fourier-transform infrared spectroscopy, thermal analyses, and field-emission scanning electron microscopy. The results indicated the formation of hydroxyapatite with 8–25 wt% of organic content, depending on the grafting method. Protein adsorption, cell adhesion, and viability studies were carried out to evaluate biological properties of grafted materials. The viability of MG-63 human osteoblastic cells following 24 h incubation with the alanine-grafted hydroxyapatite samples is well preserved, being in all cases above the viability of cells incubated with hydroxyapatite. The alanine-grafted hydroxyapatite prepared in situ and by simple mixture showed higher protein adsorption and cell adhesion, respectively, indicating their potential toward use in regenerative medicine. |
| first_indexed | 2024-03-09T11:56:40Z |
| format | Article |
| id | doaj.art-80247f1dff144a49a9b53ff575ff9822 |
| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-09T11:56:40Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj.art-80247f1dff144a49a9b53ff575ff98222023-11-30T23:08:15ZengMDPI AGLife2075-17292022-12-0113111610.3390/life13010116Synthesis and Biological Properties of Alanine-Grafted Hydroxyapatite NanoparticlesBruna Carolina Dorm0Mônica Rosas Costa Iemma1Benedito Domingos Neto2Rauany Cristina Lopes Francisco3Ivana Dinić4Nenad Ignjatović5Smilja Marković6Marina Vuković7Srečo Škapin8Eliane Trovatti9Lidija Mančić10Department of Health and Biological Sciences,Universidade de Araraquara-UNIARA, CEP, Araraquara 14801-340, BrazilDepartment of Health and Biological Sciences,Universidade de Araraquara-UNIARA, CEP, Araraquara 14801-340, BrazilDepartment of Health and Biological Sciences,Universidade de Araraquara-UNIARA, CEP, Araraquara 14801-340, BrazilDepartment of Health and Biological Sciences,Universidade de Araraquara-UNIARA, CEP, Araraquara 14801-340, BrazilInstitute of Technical Sciences of SASA, P.O. Box 377, 11000 Belgrade, SerbiaInstitute of Technical Sciences of SASA, P.O. Box 377, 11000 Belgrade, SerbiaInstitute of Technical Sciences of SASA, P.O. Box 377, 11000 Belgrade, SerbiaInnovative Centre, Faculty of Chemistry, University of Belgrade, P.O. Box 51 Belgrade, SerbiaJožef Stefan Institute, P.O. Box 3000 Ljubljana, SloveniaDepartment of Health and Biological Sciences,Universidade de Araraquara-UNIARA, CEP, Araraquara 14801-340, BrazilInstitute of Technical Sciences of SASA, P.O. Box 377, 11000 Belgrade, SerbiaHydroxyapatite attracts great attention as hard tissues implant material for bones and teeth. Its application in reconstructive medicine depends on its biocompatibility, which is in a function of composition and surface properties. The insertion of a protein element in the composition of implants can improve the cell adhesion and the osseointegration. Having this in mind, the proposal of this work was to develop L-alanine-grafted hydroxyapatite nanoparticles and to study their biocompatibility. Two L-alanine sources and three grafting methods were used for hydroxyapatite surface functionalization. The efficiency of grafting was determined based on X-ray powder diffraction, Fourier-transform infrared spectroscopy, thermal analyses, and field-emission scanning electron microscopy. The results indicated the formation of hydroxyapatite with 8–25 wt% of organic content, depending on the grafting method. Protein adsorption, cell adhesion, and viability studies were carried out to evaluate biological properties of grafted materials. The viability of MG-63 human osteoblastic cells following 24 h incubation with the alanine-grafted hydroxyapatite samples is well preserved, being in all cases above the viability of cells incubated with hydroxyapatite. The alanine-grafted hydroxyapatite prepared in situ and by simple mixture showed higher protein adsorption and cell adhesion, respectively, indicating their potential toward use in regenerative medicine.https://www.mdpi.com/2075-1729/13/1/116hydroxyapatitealanineamino acidsurface modificationcell adhesioncell viability |
| spellingShingle | Bruna Carolina Dorm Mônica Rosas Costa Iemma Benedito Domingos Neto Rauany Cristina Lopes Francisco Ivana Dinić Nenad Ignjatović Smilja Marković Marina Vuković Srečo Škapin Eliane Trovatti Lidija Mančić Synthesis and Biological Properties of Alanine-Grafted Hydroxyapatite Nanoparticles Life hydroxyapatite alanine amino acid surface modification cell adhesion cell viability |
| title | Synthesis and Biological Properties of Alanine-Grafted Hydroxyapatite Nanoparticles |
| title_full | Synthesis and Biological Properties of Alanine-Grafted Hydroxyapatite Nanoparticles |
| title_fullStr | Synthesis and Biological Properties of Alanine-Grafted Hydroxyapatite Nanoparticles |
| title_full_unstemmed | Synthesis and Biological Properties of Alanine-Grafted Hydroxyapatite Nanoparticles |
| title_short | Synthesis and Biological Properties of Alanine-Grafted Hydroxyapatite Nanoparticles |
| title_sort | synthesis and biological properties of alanine grafted hydroxyapatite nanoparticles |
| topic | hydroxyapatite alanine amino acid surface modification cell adhesion cell viability |
| url | https://www.mdpi.com/2075-1729/13/1/116 |
| work_keys_str_mv | AT brunacarolinadorm synthesisandbiologicalpropertiesofalaninegraftedhydroxyapatitenanoparticles AT monicarosascostaiemma synthesisandbiologicalpropertiesofalaninegraftedhydroxyapatitenanoparticles AT beneditodomingosneto synthesisandbiologicalpropertiesofalaninegraftedhydroxyapatitenanoparticles AT rauanycristinalopesfrancisco synthesisandbiologicalpropertiesofalaninegraftedhydroxyapatitenanoparticles AT ivanadinic synthesisandbiologicalpropertiesofalaninegraftedhydroxyapatitenanoparticles AT nenadignjatovic synthesisandbiologicalpropertiesofalaninegraftedhydroxyapatitenanoparticles AT smiljamarkovic synthesisandbiologicalpropertiesofalaninegraftedhydroxyapatitenanoparticles AT marinavukovic synthesisandbiologicalpropertiesofalaninegraftedhydroxyapatitenanoparticles AT srecoskapin synthesisandbiologicalpropertiesofalaninegraftedhydroxyapatitenanoparticles AT elianetrovatti synthesisandbiologicalpropertiesofalaninegraftedhydroxyapatitenanoparticles AT lidijamancic synthesisandbiologicalpropertiesofalaninegraftedhydroxyapatitenanoparticles |