A Novel <i>Perilla frutescens</i> (L.) Britton Cell-Derived Phytocomplex Regulates Keratinocytes Inflammatory Cascade and Barrier Function and Preserves Vaginal Mucosal Integrity In Vivo
In the last years, the medicinal plant <i>Perilla frutescens</i> (L.) Britton has gained scientific interest because leaf extracts, due to the presence of rosmarinic acid and other polyphenols, have shown anti-allergic and skin protective potential in pre-clinical studies. Nevertheless,...
| Main Authors: | , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-01-01
|
| Series: | Pharmaceutics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1999-4923/15/1/240 |
| _version_ | 1797437786396033024 |
|---|---|
| author | Giovanna Pressi Giovanna Rigillo Paolo Governa Vittoria Borgonetti Giulia Baini Raffaella Rizzi Chiara Guarnerio Oriana Bertaiola Marco Frigo Matilde Merlin Stefania Paltrinieri Roberto Zambonin Stefano Pandolfo Marco Biagi |
| author_facet | Giovanna Pressi Giovanna Rigillo Paolo Governa Vittoria Borgonetti Giulia Baini Raffaella Rizzi Chiara Guarnerio Oriana Bertaiola Marco Frigo Matilde Merlin Stefania Paltrinieri Roberto Zambonin Stefano Pandolfo Marco Biagi |
| author_sort | Giovanna Pressi |
| collection | DOAJ |
| description | In the last years, the medicinal plant <i>Perilla frutescens</i> (L.) Britton has gained scientific interest because leaf extracts, due to the presence of rosmarinic acid and other polyphenols, have shown anti-allergic and skin protective potential in pre-clinical studies. Nevertheless, the lack of standardized extracts has limited clinical applications to date. In this work, for the first time, a standardized phytocomplex of <i>P. frutescens</i>, enriched in rosmarinic acid and total polyphenols, was produced through innovative in vitro cell culture biotechnology and tested. The activity of perilla was evaluated in an in vitro inflammatory model of human keratinocytes (HaCaT) by monitoring tight junctions, filaggrin, and loricrin protein levels, the release of pro-inflammatory cytokines and JNK MAPK signaling. In a practical health care application, the perilla biotechnological phytocomplex was tested in a multilayer model of vaginal mucosa, and then, in a preliminary clinical observation to explore its capacity to preserve vaginal mucosal integrity in women in peri-menopause. In keratinocytes cells, perilla phytocomplex demonstrated to exert a marked activity in epidermis barrier maintenance and anti-inflammatory effects, preserving tight junction expression and downregulating cytokines release through targeting JNK activation. Furthermore, perilla showed positive effects in retaining vaginal mucosal integrity in the reconstructed vaginal mucosa model and in vivo tests. Overall, our data suggest that the biotechnological <i>P. frutescens</i> phytocomplex could represent an innovative ingredient for dermatological applications. |
| first_indexed | 2024-03-09T11:27:39Z |
| format | Article |
| id | doaj.art-80281b600d49423e967986ed6263d3b6 |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-09T11:27:39Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-80281b600d49423e967986ed6263d3b62023-11-30T23:59:39ZengMDPI AGPharmaceutics1999-49232023-01-0115124010.3390/pharmaceutics15010240A Novel <i>Perilla frutescens</i> (L.) Britton Cell-Derived Phytocomplex Regulates Keratinocytes Inflammatory Cascade and Barrier Function and Preserves Vaginal Mucosal Integrity In VivoGiovanna Pressi0Giovanna Rigillo1Paolo Governa2Vittoria Borgonetti3Giulia Baini4Raffaella Rizzi5Chiara Guarnerio6Oriana Bertaiola7Marco Frigo8Matilde Merlin9Stefania Paltrinieri10Roberto Zambonin11Stefano Pandolfo12Marco Biagi13Aethera Biotech s.r.l., 36043 Camisano Vicentino, ItalyDepartment of Biomedical, Metabolic and Neural Science, University of Modena and Reggio Emilia, 41125 Modena, ItalyDepartment of Biotechnology, Chemistry and Pharmacy (Department of Excellence 2018–2022), University of Siena, 53100 Siena, ItalyDepartment of Neuroscience, Psychology, Drug Research and Child Health (NEUROFARBA), University of Florence, 50139 Florence, ItalyDepartment of Physical Sciences, Earth and Environment, University of Siena, 53100 Siena, ItalyAethera Biotech s.r.l., 36043 Camisano Vicentino, ItalyAethera Biotech s.r.l., 36043 Camisano Vicentino, ItalyAethera Biotech s.r.l., 36043 Camisano Vicentino, ItalyAethera Biotech s.r.l., 36043 Camisano Vicentino, ItalyAethera Biotech s.r.l., 36043 Camisano Vicentino, ItalyAethera Biotech s.r.l., 36043 Camisano Vicentino, ItalyEurochem Ricerche s.r.l., 35035 Mestrino, ItalyEurochem Ricerche s.r.l., 35035 Mestrino, ItalyDepartment of Physical Sciences, Earth and Environment, University of Siena, 53100 Siena, ItalyIn the last years, the medicinal plant <i>Perilla frutescens</i> (L.) Britton has gained scientific interest because leaf extracts, due to the presence of rosmarinic acid and other polyphenols, have shown anti-allergic and skin protective potential in pre-clinical studies. Nevertheless, the lack of standardized extracts has limited clinical applications to date. In this work, for the first time, a standardized phytocomplex of <i>P. frutescens</i>, enriched in rosmarinic acid and total polyphenols, was produced through innovative in vitro cell culture biotechnology and tested. The activity of perilla was evaluated in an in vitro inflammatory model of human keratinocytes (HaCaT) by monitoring tight junctions, filaggrin, and loricrin protein levels, the release of pro-inflammatory cytokines and JNK MAPK signaling. In a practical health care application, the perilla biotechnological phytocomplex was tested in a multilayer model of vaginal mucosa, and then, in a preliminary clinical observation to explore its capacity to preserve vaginal mucosal integrity in women in peri-menopause. In keratinocytes cells, perilla phytocomplex demonstrated to exert a marked activity in epidermis barrier maintenance and anti-inflammatory effects, preserving tight junction expression and downregulating cytokines release through targeting JNK activation. Furthermore, perilla showed positive effects in retaining vaginal mucosal integrity in the reconstructed vaginal mucosa model and in vivo tests. Overall, our data suggest that the biotechnological <i>P. frutescens</i> phytocomplex could represent an innovative ingredient for dermatological applications.https://www.mdpi.com/1999-4923/15/1/240<i>Perilla frutescens</i>rosmarinic acidphytocomplexskin integrityinflammation |
| spellingShingle | Giovanna Pressi Giovanna Rigillo Paolo Governa Vittoria Borgonetti Giulia Baini Raffaella Rizzi Chiara Guarnerio Oriana Bertaiola Marco Frigo Matilde Merlin Stefania Paltrinieri Roberto Zambonin Stefano Pandolfo Marco Biagi A Novel <i>Perilla frutescens</i> (L.) Britton Cell-Derived Phytocomplex Regulates Keratinocytes Inflammatory Cascade and Barrier Function and Preserves Vaginal Mucosal Integrity In Vivo Pharmaceutics <i>Perilla frutescens</i> rosmarinic acid phytocomplex skin integrity inflammation |
| title | A Novel <i>Perilla frutescens</i> (L.) Britton Cell-Derived Phytocomplex Regulates Keratinocytes Inflammatory Cascade and Barrier Function and Preserves Vaginal Mucosal Integrity In Vivo |
| title_full | A Novel <i>Perilla frutescens</i> (L.) Britton Cell-Derived Phytocomplex Regulates Keratinocytes Inflammatory Cascade and Barrier Function and Preserves Vaginal Mucosal Integrity In Vivo |
| title_fullStr | A Novel <i>Perilla frutescens</i> (L.) Britton Cell-Derived Phytocomplex Regulates Keratinocytes Inflammatory Cascade and Barrier Function and Preserves Vaginal Mucosal Integrity In Vivo |
| title_full_unstemmed | A Novel <i>Perilla frutescens</i> (L.) Britton Cell-Derived Phytocomplex Regulates Keratinocytes Inflammatory Cascade and Barrier Function and Preserves Vaginal Mucosal Integrity In Vivo |
| title_short | A Novel <i>Perilla frutescens</i> (L.) Britton Cell-Derived Phytocomplex Regulates Keratinocytes Inflammatory Cascade and Barrier Function and Preserves Vaginal Mucosal Integrity In Vivo |
| title_sort | novel i perilla frutescens i l britton cell derived phytocomplex regulates keratinocytes inflammatory cascade and barrier function and preserves vaginal mucosal integrity in vivo |
| topic | <i>Perilla frutescens</i> rosmarinic acid phytocomplex skin integrity inflammation |
| url | https://www.mdpi.com/1999-4923/15/1/240 |
| work_keys_str_mv | AT giovannapressi anoveliperillafrutescensilbrittoncellderivedphytocomplexregulateskeratinocytesinflammatorycascadeandbarrierfunctionandpreservesvaginalmucosalintegrityinvivo AT giovannarigillo anoveliperillafrutescensilbrittoncellderivedphytocomplexregulateskeratinocytesinflammatorycascadeandbarrierfunctionandpreservesvaginalmucosalintegrityinvivo AT paologoverna anoveliperillafrutescensilbrittoncellderivedphytocomplexregulateskeratinocytesinflammatorycascadeandbarrierfunctionandpreservesvaginalmucosalintegrityinvivo AT vittoriaborgonetti anoveliperillafrutescensilbrittoncellderivedphytocomplexregulateskeratinocytesinflammatorycascadeandbarrierfunctionandpreservesvaginalmucosalintegrityinvivo AT giuliabaini anoveliperillafrutescensilbrittoncellderivedphytocomplexregulateskeratinocytesinflammatorycascadeandbarrierfunctionandpreservesvaginalmucosalintegrityinvivo AT raffaellarizzi anoveliperillafrutescensilbrittoncellderivedphytocomplexregulateskeratinocytesinflammatorycascadeandbarrierfunctionandpreservesvaginalmucosalintegrityinvivo AT chiaraguarnerio anoveliperillafrutescensilbrittoncellderivedphytocomplexregulateskeratinocytesinflammatorycascadeandbarrierfunctionandpreservesvaginalmucosalintegrityinvivo AT orianabertaiola anoveliperillafrutescensilbrittoncellderivedphytocomplexregulateskeratinocytesinflammatorycascadeandbarrierfunctionandpreservesvaginalmucosalintegrityinvivo AT marcofrigo anoveliperillafrutescensilbrittoncellderivedphytocomplexregulateskeratinocytesinflammatorycascadeandbarrierfunctionandpreservesvaginalmucosalintegrityinvivo AT matildemerlin anoveliperillafrutescensilbrittoncellderivedphytocomplexregulateskeratinocytesinflammatorycascadeandbarrierfunctionandpreservesvaginalmucosalintegrityinvivo AT stefaniapaltrinieri anoveliperillafrutescensilbrittoncellderivedphytocomplexregulateskeratinocytesinflammatorycascadeandbarrierfunctionandpreservesvaginalmucosalintegrityinvivo AT robertozambonin anoveliperillafrutescensilbrittoncellderivedphytocomplexregulateskeratinocytesinflammatorycascadeandbarrierfunctionandpreservesvaginalmucosalintegrityinvivo AT stefanopandolfo anoveliperillafrutescensilbrittoncellderivedphytocomplexregulateskeratinocytesinflammatorycascadeandbarrierfunctionandpreservesvaginalmucosalintegrityinvivo AT marcobiagi anoveliperillafrutescensilbrittoncellderivedphytocomplexregulateskeratinocytesinflammatorycascadeandbarrierfunctionandpreservesvaginalmucosalintegrityinvivo AT giovannapressi noveliperillafrutescensilbrittoncellderivedphytocomplexregulateskeratinocytesinflammatorycascadeandbarrierfunctionandpreservesvaginalmucosalintegrityinvivo AT giovannarigillo noveliperillafrutescensilbrittoncellderivedphytocomplexregulateskeratinocytesinflammatorycascadeandbarrierfunctionandpreservesvaginalmucosalintegrityinvivo AT paologoverna noveliperillafrutescensilbrittoncellderivedphytocomplexregulateskeratinocytesinflammatorycascadeandbarrierfunctionandpreservesvaginalmucosalintegrityinvivo AT vittoriaborgonetti noveliperillafrutescensilbrittoncellderivedphytocomplexregulateskeratinocytesinflammatorycascadeandbarrierfunctionandpreservesvaginalmucosalintegrityinvivo AT giuliabaini noveliperillafrutescensilbrittoncellderivedphytocomplexregulateskeratinocytesinflammatorycascadeandbarrierfunctionandpreservesvaginalmucosalintegrityinvivo AT raffaellarizzi noveliperillafrutescensilbrittoncellderivedphytocomplexregulateskeratinocytesinflammatorycascadeandbarrierfunctionandpreservesvaginalmucosalintegrityinvivo AT chiaraguarnerio noveliperillafrutescensilbrittoncellderivedphytocomplexregulateskeratinocytesinflammatorycascadeandbarrierfunctionandpreservesvaginalmucosalintegrityinvivo AT orianabertaiola noveliperillafrutescensilbrittoncellderivedphytocomplexregulateskeratinocytesinflammatorycascadeandbarrierfunctionandpreservesvaginalmucosalintegrityinvivo AT marcofrigo noveliperillafrutescensilbrittoncellderivedphytocomplexregulateskeratinocytesinflammatorycascadeandbarrierfunctionandpreservesvaginalmucosalintegrityinvivo AT matildemerlin noveliperillafrutescensilbrittoncellderivedphytocomplexregulateskeratinocytesinflammatorycascadeandbarrierfunctionandpreservesvaginalmucosalintegrityinvivo AT stefaniapaltrinieri noveliperillafrutescensilbrittoncellderivedphytocomplexregulateskeratinocytesinflammatorycascadeandbarrierfunctionandpreservesvaginalmucosalintegrityinvivo AT robertozambonin noveliperillafrutescensilbrittoncellderivedphytocomplexregulateskeratinocytesinflammatorycascadeandbarrierfunctionandpreservesvaginalmucosalintegrityinvivo AT stefanopandolfo noveliperillafrutescensilbrittoncellderivedphytocomplexregulateskeratinocytesinflammatorycascadeandbarrierfunctionandpreservesvaginalmucosalintegrityinvivo AT marcobiagi noveliperillafrutescensilbrittoncellderivedphytocomplexregulateskeratinocytesinflammatorycascadeandbarrierfunctionandpreservesvaginalmucosalintegrityinvivo |