Postbiotic Fractions of Probiotics <i>Lactobacillus plantarum</i> 299v and <i>Lactobacillus rhamnosus</i> GG Show Immune-Modulating Effects
Probiotic bacteria belonging to <i>Lactobacillus</i> spp. are important producers of bioactive molecules, known as postbiotics, that play essential roles in the immunological support of the intestinal mucosa. In this study, the system of co-culture of intestinal epithelial cells with mac...
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| Format: | Article |
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MDPI AG
2023-10-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/12/21/2538 |
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| author | Agnieszka Magryś Mateusz Pawlik |
| author_facet | Agnieszka Magryś Mateusz Pawlik |
| author_sort | Agnieszka Magryś |
| collection | DOAJ |
| description | Probiotic bacteria belonging to <i>Lactobacillus</i> spp. are important producers of bioactive molecules, known as postbiotics, that play essential roles in the immunological support of the intestinal mucosa. In this study, the system of co-culture of intestinal epithelial cells with macrophage cells in vitro was used to study the potential effect of postbiotic fractions of <i>L. rhamonosus</i> and <i>L. plantarum</i> on the modulation of the immune response induced by pro-inflammatory stimuli. This study’s results revealed that the presence of probiotic bacterial components on the mucosal surface in the early and late stage of inflammatory conditions is based on cellular interactions that control inflammation and consequent damage to the intestinal epithelium. In our studies, heat killed fractions of probiotic bacteria and their extracted proteins showed a beneficial effect on controlling inflammation, regardless of the strain tested, consequently protecting intestinal barrier damage. In conclusion, the presented results emphasize that the fractions of probiotic bacteria of <i>L. plantarum</i> and <i>L. rhamnosus</i> may play a significant role in the regulation of LPS-mediated cytotoxic activity in intestinal epithelial cells. The fractions of probiotic strains of <i>L. rhamnosus</i> and <i>L. plantarum</i> showed the potential to suppress inflammation, effectively activating the anti-inflammatory cytokine IL-10 and modulating the IL-18-related response. |
| first_indexed | 2024-03-11T11:32:43Z |
| format | Article |
| id | doaj.art-80285859143e4e759954fed6fd8ea788 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-11T11:32:43Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-80285859143e4e759954fed6fd8ea7882023-11-10T15:00:42ZengMDPI AGCells2073-44092023-10-011221253810.3390/cells12212538Postbiotic Fractions of Probiotics <i>Lactobacillus plantarum</i> 299v and <i>Lactobacillus rhamnosus</i> GG Show Immune-Modulating EffectsAgnieszka Magryś0Mateusz Pawlik1Chair and Department of Medical Microbiology, Medical University of Lublin, ul. Chodźki 1, 20-093 Lublin, PolandChair and Department of Medical Microbiology, Medical University of Lublin, ul. Chodźki 1, 20-093 Lublin, PolandProbiotic bacteria belonging to <i>Lactobacillus</i> spp. are important producers of bioactive molecules, known as postbiotics, that play essential roles in the immunological support of the intestinal mucosa. In this study, the system of co-culture of intestinal epithelial cells with macrophage cells in vitro was used to study the potential effect of postbiotic fractions of <i>L. rhamonosus</i> and <i>L. plantarum</i> on the modulation of the immune response induced by pro-inflammatory stimuli. This study’s results revealed that the presence of probiotic bacterial components on the mucosal surface in the early and late stage of inflammatory conditions is based on cellular interactions that control inflammation and consequent damage to the intestinal epithelium. In our studies, heat killed fractions of probiotic bacteria and their extracted proteins showed a beneficial effect on controlling inflammation, regardless of the strain tested, consequently protecting intestinal barrier damage. In conclusion, the presented results emphasize that the fractions of probiotic bacteria of <i>L. plantarum</i> and <i>L. rhamnosus</i> may play a significant role in the regulation of LPS-mediated cytotoxic activity in intestinal epithelial cells. The fractions of probiotic strains of <i>L. rhamnosus</i> and <i>L. plantarum</i> showed the potential to suppress inflammation, effectively activating the anti-inflammatory cytokine IL-10 and modulating the IL-18-related response.https://www.mdpi.com/2073-4409/12/21/2538probioticsgut inflammationco-culture<i>Lactobacillus plantarum</i> 299v<i>Lactobacillus rhamnosus</i> GGpostbiotics |
| spellingShingle | Agnieszka Magryś Mateusz Pawlik Postbiotic Fractions of Probiotics <i>Lactobacillus plantarum</i> 299v and <i>Lactobacillus rhamnosus</i> GG Show Immune-Modulating Effects Cells probiotics gut inflammation co-culture <i>Lactobacillus plantarum</i> 299v <i>Lactobacillus rhamnosus</i> GG postbiotics |
| title | Postbiotic Fractions of Probiotics <i>Lactobacillus plantarum</i> 299v and <i>Lactobacillus rhamnosus</i> GG Show Immune-Modulating Effects |
| title_full | Postbiotic Fractions of Probiotics <i>Lactobacillus plantarum</i> 299v and <i>Lactobacillus rhamnosus</i> GG Show Immune-Modulating Effects |
| title_fullStr | Postbiotic Fractions of Probiotics <i>Lactobacillus plantarum</i> 299v and <i>Lactobacillus rhamnosus</i> GG Show Immune-Modulating Effects |
| title_full_unstemmed | Postbiotic Fractions of Probiotics <i>Lactobacillus plantarum</i> 299v and <i>Lactobacillus rhamnosus</i> GG Show Immune-Modulating Effects |
| title_short | Postbiotic Fractions of Probiotics <i>Lactobacillus plantarum</i> 299v and <i>Lactobacillus rhamnosus</i> GG Show Immune-Modulating Effects |
| title_sort | postbiotic fractions of probiotics i lactobacillus plantarum i 299v and i lactobacillus rhamnosus i gg show immune modulating effects |
| topic | probiotics gut inflammation co-culture <i>Lactobacillus plantarum</i> 299v <i>Lactobacillus rhamnosus</i> GG postbiotics |
| url | https://www.mdpi.com/2073-4409/12/21/2538 |
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