(1→3)-α-<span style="font-variant: small-caps">d</span>-Glucooligosaccharides Increase the Killing Capacity of NK Cells against Selected Human Colon Cancer Cells
Despite the progress of medicine, colorectal cancer has occupied one of the highest positions in the rankings of cancer morbidity and mortality for many years. Thus, alternative methods of its treatment are sought. One of the newer therapeutic strategies is immunotherapy based on NK cells (natural k...
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MDPI AG
2023-05-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/28/10/4212 |
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| author | Marta Kinga Lemieszek Paulina Adamczyk Iwona Komaniecka Wojciech Rzeski Michał Tomczyk Adrian Wiater |
| author_facet | Marta Kinga Lemieszek Paulina Adamczyk Iwona Komaniecka Wojciech Rzeski Michał Tomczyk Adrian Wiater |
| author_sort | Marta Kinga Lemieszek |
| collection | DOAJ |
| description | Despite the progress of medicine, colorectal cancer has occupied one of the highest positions in the rankings of cancer morbidity and mortality for many years. Thus, alternative methods of its treatment are sought. One of the newer therapeutic strategies is immunotherapy based on NK cells (natural killers), which are the body’s first line of defense against cancer. The aim of the study was to verify the possibility of using (1→3)-α-<span style="font-variant: small-caps;">d</span>-glucooligosaccharides (GOSs) obtained via acid hydrolysis of (1→3)-α-<span style="font-variant: small-caps;">d</span>-glucan from the fruiting body of <i>Laetiporus sulphureus</i> to improve the anticancer effect of NK-92 cells, with proven clinical utility, against selected human colon adenocarcinoma cell lines LS180 and HT-29. The study revealed that the investigated oligosaccharides significantly enhanced the ability of NK-92 cells to eliminate the examined colon cancer cells, mostly by an increase in their cytotoxic activity. The most significant effect was observed in LS180 and HT-29 cells exposed to a two-times higher quantity of NK cells activated by 500 µg/mL GOS, wherein NK-92 killing properties increased for 20.5% (<i>p</i> < 0.001) and 24.8% (<i>p</i> < 0.001), respectively. The beneficial impact of (1→3)-α-<span style="font-variant: small-caps;">d</span>-glucooligosaccharides on the anticancer properties of NK-92 suggests their use in colon cancer immunotherapy as adjuvants; however, the obtained data require further investigation and confirmation. |
| first_indexed | 2024-03-11T03:26:32Z |
| format | Article |
| id | doaj.art-802af4fb89ae466c87ea30fe5f81caad |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-11T03:26:32Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-802af4fb89ae466c87ea30fe5f81caad2023-11-18T02:40:57ZengMDPI AGMolecules1420-30492023-05-012810421210.3390/molecules28104212(1→3)-α-<span style="font-variant: small-caps">d</span>-Glucooligosaccharides Increase the Killing Capacity of NK Cells against Selected Human Colon Cancer CellsMarta Kinga Lemieszek0Paulina Adamczyk1Iwona Komaniecka2Wojciech Rzeski3Michał Tomczyk4Adrian Wiater5Department of Medical Biology, Institute of Rural Health, ul. Jaczewskiego 2, 20-090 Lublin, PolandDepartment of Industrial and Environmental Microbiology, Institute of Biological Science, Maria Curie-Skłodowska University, ul. Akademicka 19, 20-033 Lublin, PolandDepartment of Genetics and Microbiology, Institute of Biological Science, Maria Curie-Skłodowska University, ul. Akademicka 19, 20-033 Lublin, PolandDepartment of Medical Biology, Institute of Rural Health, ul. Jaczewskiego 2, 20-090 Lublin, PolandDepartment of Pharmacognosy, Faculty of Pharmacy with the Division of Laboratory Medicine, Medical University of Białystok, ul. Mickiewicza 2a, 15-230 Białystok, PolandDepartment of Industrial and Environmental Microbiology, Institute of Biological Science, Maria Curie-Skłodowska University, ul. Akademicka 19, 20-033 Lublin, PolandDespite the progress of medicine, colorectal cancer has occupied one of the highest positions in the rankings of cancer morbidity and mortality for many years. Thus, alternative methods of its treatment are sought. One of the newer therapeutic strategies is immunotherapy based on NK cells (natural killers), which are the body’s first line of defense against cancer. The aim of the study was to verify the possibility of using (1→3)-α-<span style="font-variant: small-caps;">d</span>-glucooligosaccharides (GOSs) obtained via acid hydrolysis of (1→3)-α-<span style="font-variant: small-caps;">d</span>-glucan from the fruiting body of <i>Laetiporus sulphureus</i> to improve the anticancer effect of NK-92 cells, with proven clinical utility, against selected human colon adenocarcinoma cell lines LS180 and HT-29. The study revealed that the investigated oligosaccharides significantly enhanced the ability of NK-92 cells to eliminate the examined colon cancer cells, mostly by an increase in their cytotoxic activity. The most significant effect was observed in LS180 and HT-29 cells exposed to a two-times higher quantity of NK cells activated by 500 µg/mL GOS, wherein NK-92 killing properties increased for 20.5% (<i>p</i> < 0.001) and 24.8% (<i>p</i> < 0.001), respectively. The beneficial impact of (1→3)-α-<span style="font-variant: small-caps;">d</span>-glucooligosaccharides on the anticancer properties of NK-92 suggests their use in colon cancer immunotherapy as adjuvants; however, the obtained data require further investigation and confirmation.https://www.mdpi.com/1420-3049/28/10/4212(1→3)-α-<span style="font-variant: small-caps">d</span>-glucooligosaccharidesimmunomodulationcancer immunotherapycolon cancerNK cells |
| spellingShingle | Marta Kinga Lemieszek Paulina Adamczyk Iwona Komaniecka Wojciech Rzeski Michał Tomczyk Adrian Wiater (1→3)-α-<span style="font-variant: small-caps">d</span>-Glucooligosaccharides Increase the Killing Capacity of NK Cells against Selected Human Colon Cancer Cells Molecules (1→3)-α-<span style="font-variant: small-caps">d</span>-glucooligosaccharides immunomodulation cancer immunotherapy colon cancer NK cells |
| title | (1→3)-α-<span style="font-variant: small-caps">d</span>-Glucooligosaccharides Increase the Killing Capacity of NK Cells against Selected Human Colon Cancer Cells |
| title_full | (1→3)-α-<span style="font-variant: small-caps">d</span>-Glucooligosaccharides Increase the Killing Capacity of NK Cells against Selected Human Colon Cancer Cells |
| title_fullStr | (1→3)-α-<span style="font-variant: small-caps">d</span>-Glucooligosaccharides Increase the Killing Capacity of NK Cells against Selected Human Colon Cancer Cells |
| title_full_unstemmed | (1→3)-α-<span style="font-variant: small-caps">d</span>-Glucooligosaccharides Increase the Killing Capacity of NK Cells against Selected Human Colon Cancer Cells |
| title_short | (1→3)-α-<span style="font-variant: small-caps">d</span>-Glucooligosaccharides Increase the Killing Capacity of NK Cells against Selected Human Colon Cancer Cells |
| title_sort | 1 3 α span style font variant small caps d span glucooligosaccharides increase the killing capacity of nk cells against selected human colon cancer cells |
| topic | (1→3)-α-<span style="font-variant: small-caps">d</span>-glucooligosaccharides immunomodulation cancer immunotherapy colon cancer NK cells |
| url | https://www.mdpi.com/1420-3049/28/10/4212 |
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