Regulation of Neural Differentiation of ADMSCs using Graphene‐Mediated Wireless‐Localized Electrical Signals Driven by Electromagnetic Induction
Abstract Although adipose‐derived mesenchymal stem cells (ADMSCs) isolated from patients’ fat are considered as the most important autologous stem cells for tissue repair, significant difficulties in the neural differentiation of ADMSCs still impede stem cell therapy for neurodegenerative diseases....
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2022-05-01
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| Series: | Advanced Science |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/advs.202104424 |
| _version_ | 1811235360716357632 |
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| author | Zhijie Guo Chunhui Sun Hongru Yang Haoyang Gao Na Liang Jian Wang Shuang Hu Na Ren Jinbo Pang Jingang Wang Ning Meng Lin Han Hong Liu |
| author_facet | Zhijie Guo Chunhui Sun Hongru Yang Haoyang Gao Na Liang Jian Wang Shuang Hu Na Ren Jinbo Pang Jingang Wang Ning Meng Lin Han Hong Liu |
| author_sort | Zhijie Guo |
| collection | DOAJ |
| description | Abstract Although adipose‐derived mesenchymal stem cells (ADMSCs) isolated from patients’ fat are considered as the most important autologous stem cells for tissue repair, significant difficulties in the neural differentiation of ADMSCs still impede stem cell therapy for neurodegenerative diseases. Herein, a wireless‐electrical stimulation method is proposed to direct the neural differentiation of ADMSCs based on the electromagnetic effect using a graphene film as a conductive scaffold. By placing a rotating magnet on the top of a culture system without any inducer, the ADMSCs cultured on graphene differentiate into functional neurons within 15 days. As a conductive biodegradable nanomaterial, graphene film acts as a wireless electrical signal generator driven by the electromagnetic induction, and millivolt‐level voltage generated in situ provokes ADMSCs to differentiate into neurons, proved by morphological variation, extremely high levels of neuron‐specific genes, and proteins. Most importantly, Ca2+ intracellular influx is observed in these ADMSC‐derived neurons once exposure to neurotransmitters, indicating that these cells are functional neurons. This research enhances stem cell therapy for neurodegenerative diseases using autologous ADMSCs and overcomes the lack of neural stem cells. This nanostructure‐mediated physical‐signal simulation method is inexpensive, safe, and localized, and has a significant impact on neural regeneration. |
| first_indexed | 2024-04-12T11:49:32Z |
| format | Article |
| id | doaj.art-80303766d24f432998f1846f85325c5d |
| institution | Directory Open Access Journal |
| issn | 2198-3844 |
| language | English |
| last_indexed | 2024-04-12T11:49:32Z |
| publishDate | 2022-05-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advanced Science |
| spelling | doaj.art-80303766d24f432998f1846f85325c5d2022-12-22T03:34:13ZengWileyAdvanced Science2198-38442022-05-01914n/an/a10.1002/advs.202104424Regulation of Neural Differentiation of ADMSCs using Graphene‐Mediated Wireless‐Localized Electrical Signals Driven by Electromagnetic InductionZhijie Guo0Chunhui Sun1Hongru Yang2Haoyang Gao3Na Liang4Jian Wang5Shuang Hu6Na Ren7Jinbo Pang8Jingang Wang9Ning Meng10Lin Han11Hong Liu12Collaborative Innovation Center of Technology and Equipment for Biological Diagnosis and Therapy in Universities of Shandong Institute for Advanced Interdisciplinary Research (iAIR) University of Jinan Jinan 250022 P. R. ChinaCollaborative Innovation Center of Technology and Equipment for Biological Diagnosis and Therapy in Universities of Shandong Institute for Advanced Interdisciplinary Research (iAIR) University of Jinan Jinan 250022 P. R. ChinaState Key Laboratory of Crystal Materials Shandong University Jinan Shandong 250100 P. R. ChinaCollaborative Innovation Center of Technology and Equipment for Biological Diagnosis and Therapy in Universities of Shandong Institute for Advanced Interdisciplinary Research (iAIR) University of Jinan Jinan 250022 P. R. ChinaCollaborative Innovation Center of Technology and Equipment for Biological Diagnosis and Therapy in Universities of Shandong Institute for Advanced Interdisciplinary Research (iAIR) University of Jinan Jinan 250022 P. R. ChinaCollaborative Innovation Center of Technology and Equipment for Biological Diagnosis and Therapy in Universities of Shandong Institute for Advanced Interdisciplinary Research (iAIR) University of Jinan Jinan 250022 P. R. ChinaCollaborative Innovation Center of Technology and Equipment for Biological Diagnosis and Therapy in Universities of Shandong Institute for Advanced Interdisciplinary Research (iAIR) University of Jinan Jinan 250022 P. R. ChinaCollaborative Innovation Center of Technology and Equipment for Biological Diagnosis and Therapy in Universities of Shandong Institute for Advanced Interdisciplinary Research (iAIR) University of Jinan Jinan 250022 P. R. ChinaCollaborative Innovation Center of Technology and Equipment for Biological Diagnosis and Therapy in Universities of Shandong Institute for Advanced Interdisciplinary Research (iAIR) University of Jinan Jinan 250022 P. R. ChinaCollaborative Innovation Center of Technology and Equipment for Biological Diagnosis and Therapy in Universities of Shandong Institute for Advanced Interdisciplinary Research (iAIR) University of Jinan Jinan 250022 P. R. ChinaSchool of Biological Science and Technology University of Jinan Jinan Shandong 250022 P. R. ChinaInstitute of Marine Science and Technology Shandong University Qingdao Shandong 266200 P. R. ChinaCollaborative Innovation Center of Technology and Equipment for Biological Diagnosis and Therapy in Universities of Shandong Institute for Advanced Interdisciplinary Research (iAIR) University of Jinan Jinan 250022 P. R. ChinaAbstract Although adipose‐derived mesenchymal stem cells (ADMSCs) isolated from patients’ fat are considered as the most important autologous stem cells for tissue repair, significant difficulties in the neural differentiation of ADMSCs still impede stem cell therapy for neurodegenerative diseases. Herein, a wireless‐electrical stimulation method is proposed to direct the neural differentiation of ADMSCs based on the electromagnetic effect using a graphene film as a conductive scaffold. By placing a rotating magnet on the top of a culture system without any inducer, the ADMSCs cultured on graphene differentiate into functional neurons within 15 days. As a conductive biodegradable nanomaterial, graphene film acts as a wireless electrical signal generator driven by the electromagnetic induction, and millivolt‐level voltage generated in situ provokes ADMSCs to differentiate into neurons, proved by morphological variation, extremely high levels of neuron‐specific genes, and proteins. Most importantly, Ca2+ intracellular influx is observed in these ADMSC‐derived neurons once exposure to neurotransmitters, indicating that these cells are functional neurons. This research enhances stem cell therapy for neurodegenerative diseases using autologous ADMSCs and overcomes the lack of neural stem cells. This nanostructure‐mediated physical‐signal simulation method is inexpensive, safe, and localized, and has a significant impact on neural regeneration.https://doi.org/10.1002/advs.202104424adipose‐derived mesenchymal stem cellselectromagnetic inductiongraphene filmmagneto‐electric biomaterialneural differentiation |
| spellingShingle | Zhijie Guo Chunhui Sun Hongru Yang Haoyang Gao Na Liang Jian Wang Shuang Hu Na Ren Jinbo Pang Jingang Wang Ning Meng Lin Han Hong Liu Regulation of Neural Differentiation of ADMSCs using Graphene‐Mediated Wireless‐Localized Electrical Signals Driven by Electromagnetic Induction Advanced Science adipose‐derived mesenchymal stem cells electromagnetic induction graphene film magneto‐electric biomaterial neural differentiation |
| title | Regulation of Neural Differentiation of ADMSCs using Graphene‐Mediated Wireless‐Localized Electrical Signals Driven by Electromagnetic Induction |
| title_full | Regulation of Neural Differentiation of ADMSCs using Graphene‐Mediated Wireless‐Localized Electrical Signals Driven by Electromagnetic Induction |
| title_fullStr | Regulation of Neural Differentiation of ADMSCs using Graphene‐Mediated Wireless‐Localized Electrical Signals Driven by Electromagnetic Induction |
| title_full_unstemmed | Regulation of Neural Differentiation of ADMSCs using Graphene‐Mediated Wireless‐Localized Electrical Signals Driven by Electromagnetic Induction |
| title_short | Regulation of Neural Differentiation of ADMSCs using Graphene‐Mediated Wireless‐Localized Electrical Signals Driven by Electromagnetic Induction |
| title_sort | regulation of neural differentiation of admscs using graphene mediated wireless localized electrical signals driven by electromagnetic induction |
| topic | adipose‐derived mesenchymal stem cells electromagnetic induction graphene film magneto‐electric biomaterial neural differentiation |
| url | https://doi.org/10.1002/advs.202104424 |
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