Sulfated Lactosyl Archaeol Archaeosome-Adjuvanted Vaccine Formulations Targeting Rabbit Hemorrhagic Disease Virus Are Immunogenic and Efficacious
Vaccines play an important role in maintaining human and animal health worldwide. There is continued demand for effective and safe adjuvants capable of enhancing antigen-specific responses to a target pathogen. Rabbit hemorrhagic disease virus (RHDV) is a highly contagious calicivirus that often ind...
| Main Authors: | , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-05-01
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| Series: | Vaccines |
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| Online Access: | https://www.mdpi.com/2076-393X/11/6/1043 |
| _version_ | 1827735454646009856 |
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| author | Bassel Akache Andrew J. Read Renu Dudani Blair A. Harrison Dean Williams Lise Deschatelets Yimei Jia Vandana Chandan Felicity C. Stark Gerard Agbayani Shawn R. Makinen Usha D. Hemraz Edmond Lam Sophie Régnier Wei Zou Peter D. Kirkland Michael J. McCluskie |
| author_facet | Bassel Akache Andrew J. Read Renu Dudani Blair A. Harrison Dean Williams Lise Deschatelets Yimei Jia Vandana Chandan Felicity C. Stark Gerard Agbayani Shawn R. Makinen Usha D. Hemraz Edmond Lam Sophie Régnier Wei Zou Peter D. Kirkland Michael J. McCluskie |
| author_sort | Bassel Akache |
| collection | DOAJ |
| description | Vaccines play an important role in maintaining human and animal health worldwide. There is continued demand for effective and safe adjuvants capable of enhancing antigen-specific responses to a target pathogen. Rabbit hemorrhagic disease virus (RHDV) is a highly contagious calicivirus that often induces high mortality rates in rabbits. Herein, we evaluated the activity of an experimental sulfated lactosyl archaeol (SLA) archaeosome adjuvant when incorporated in subunit vaccine formulations targeting RHDV. The subunit antigens consisted of RHDV–CRM<sub>197</sub> peptide conjugates or recombinant RHDV2 VP60. SLA was able to enhance antigen-specific antibody titers and cellular responses in mice and rabbits. Three weeks following immunization, antigen-specific antibody levels in rabbits vaccinated with RHDV2 VP60 + SLA were significantly higher than those immunized with antigen alone, with geomean titers of 7393 vs. 117. In addition, the SLA-adjuvanted VP60-based formulations were highly efficacious in a rabbit RHDV2 challenge model with up to 87.5% animals surviving the viral challenge. These findings demonstrate the potential utility of SLA adjuvants in veterinary applications and highlight its activity in different types of mammalian species. |
| first_indexed | 2024-03-11T01:51:07Z |
| format | Article |
| id | doaj.art-8033a136f9c84c7da76024c305c13447 |
| institution | Directory Open Access Journal |
| issn | 2076-393X |
| language | English |
| last_indexed | 2024-03-11T01:51:07Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Vaccines |
| spelling | doaj.art-8033a136f9c84c7da76024c305c134472023-11-18T12:58:25ZengMDPI AGVaccines2076-393X2023-05-01116104310.3390/vaccines11061043Sulfated Lactosyl Archaeol Archaeosome-Adjuvanted Vaccine Formulations Targeting Rabbit Hemorrhagic Disease Virus Are Immunogenic and EfficaciousBassel Akache0Andrew J. Read1Renu Dudani2Blair A. Harrison3Dean Williams4Lise Deschatelets5Yimei Jia6Vandana Chandan7Felicity C. Stark8Gerard Agbayani9Shawn R. Makinen10Usha D. Hemraz11Edmond Lam12Sophie Régnier13Wei Zou14Peter D. Kirkland15Michael J. McCluskie16National Research Council Canada, Human Health Therapeutics, Ottawa, ON K1A 0R6, CanadaVirology Laboratory, Elizabeth Macarthur Agricultural Institute, NSW Department of Primary Industries, Menangle, NSW 2567, AustraliaNational Research Council Canada, Human Health Therapeutics, Ottawa, ON K1A 0R6, CanadaNational Research Council Canada, Human Health Therapeutics, Ottawa, ON K1A 0R6, CanadaNational Research Council Canada, Human Health Therapeutics, Ottawa, ON K1A 0R6, CanadaNational Research Council Canada, Human Health Therapeutics, Ottawa, ON K1A 0R6, CanadaNational Research Council Canada, Human Health Therapeutics, Ottawa, ON K1A 0R6, CanadaNational Research Council Canada, Human Health Therapeutics, Ottawa, ON K1A 0R6, CanadaNational Research Council Canada, Human Health Therapeutics, Ottawa, ON K1A 0R6, CanadaNational Research Council Canada, Human Health Therapeutics, Ottawa, ON K1A 0R6, CanadaNational Research Council Canada, Human Health Therapeutics, Ottawa, ON K1A 0R6, CanadaNational Research Council Canada, Aquatic and Crop Resource Development, Montreal, QC H4P 2R2, CanadaNational Research Council Canada, Aquatic and Crop Resource Development, Montreal, QC H4P 2R2, CanadaNational Research Council Canada, Aquatic and Crop Resource Development, Montreal, QC H4P 2R2, CanadaNational Research Council Canada, Human Health Therapeutics, Ottawa, ON K1A 0R6, CanadaVirology Laboratory, Elizabeth Macarthur Agricultural Institute, NSW Department of Primary Industries, Menangle, NSW 2567, AustraliaNational Research Council Canada, Human Health Therapeutics, Ottawa, ON K1A 0R6, CanadaVaccines play an important role in maintaining human and animal health worldwide. There is continued demand for effective and safe adjuvants capable of enhancing antigen-specific responses to a target pathogen. Rabbit hemorrhagic disease virus (RHDV) is a highly contagious calicivirus that often induces high mortality rates in rabbits. Herein, we evaluated the activity of an experimental sulfated lactosyl archaeol (SLA) archaeosome adjuvant when incorporated in subunit vaccine formulations targeting RHDV. The subunit antigens consisted of RHDV–CRM<sub>197</sub> peptide conjugates or recombinant RHDV2 VP60. SLA was able to enhance antigen-specific antibody titers and cellular responses in mice and rabbits. Three weeks following immunization, antigen-specific antibody levels in rabbits vaccinated with RHDV2 VP60 + SLA were significantly higher than those immunized with antigen alone, with geomean titers of 7393 vs. 117. In addition, the SLA-adjuvanted VP60-based formulations were highly efficacious in a rabbit RHDV2 challenge model with up to 87.5% animals surviving the viral challenge. These findings demonstrate the potential utility of SLA adjuvants in veterinary applications and highlight its activity in different types of mammalian species.https://www.mdpi.com/2076-393X/11/6/1043rabbit hemorrhagic disease virusRHDVsulfated lactosyl archaeolSLAvaccineadjuvant |
| spellingShingle | Bassel Akache Andrew J. Read Renu Dudani Blair A. Harrison Dean Williams Lise Deschatelets Yimei Jia Vandana Chandan Felicity C. Stark Gerard Agbayani Shawn R. Makinen Usha D. Hemraz Edmond Lam Sophie Régnier Wei Zou Peter D. Kirkland Michael J. McCluskie Sulfated Lactosyl Archaeol Archaeosome-Adjuvanted Vaccine Formulations Targeting Rabbit Hemorrhagic Disease Virus Are Immunogenic and Efficacious Vaccines rabbit hemorrhagic disease virus RHDV sulfated lactosyl archaeol SLA vaccine adjuvant |
| title | Sulfated Lactosyl Archaeol Archaeosome-Adjuvanted Vaccine Formulations Targeting Rabbit Hemorrhagic Disease Virus Are Immunogenic and Efficacious |
| title_full | Sulfated Lactosyl Archaeol Archaeosome-Adjuvanted Vaccine Formulations Targeting Rabbit Hemorrhagic Disease Virus Are Immunogenic and Efficacious |
| title_fullStr | Sulfated Lactosyl Archaeol Archaeosome-Adjuvanted Vaccine Formulations Targeting Rabbit Hemorrhagic Disease Virus Are Immunogenic and Efficacious |
| title_full_unstemmed | Sulfated Lactosyl Archaeol Archaeosome-Adjuvanted Vaccine Formulations Targeting Rabbit Hemorrhagic Disease Virus Are Immunogenic and Efficacious |
| title_short | Sulfated Lactosyl Archaeol Archaeosome-Adjuvanted Vaccine Formulations Targeting Rabbit Hemorrhagic Disease Virus Are Immunogenic and Efficacious |
| title_sort | sulfated lactosyl archaeol archaeosome adjuvanted vaccine formulations targeting rabbit hemorrhagic disease virus are immunogenic and efficacious |
| topic | rabbit hemorrhagic disease virus RHDV sulfated lactosyl archaeol SLA vaccine adjuvant |
| url | https://www.mdpi.com/2076-393X/11/6/1043 |
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