Resistance Exercise Training Improves Metabolic and Inflammatory Control in Adipose and Muscle Tissues in Mice Fed a High-Fat Diet
This study investigates whether ladder climbing (LC), as a model of resistance exercise, can reverse whole-body and skeletal muscle deleterious metabolic and inflammatory effects of high-fat (HF) diet-induced obesity in mice. To accomplish this, Swiss mice were fed for 17 weeks either standard chow...
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| Format: | Article |
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MDPI AG
2022-05-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/14/11/2179 |
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| author | Pauline S. Effting Anand Thirupathi Alexandre P. Müller Bárbara C. Pereira Diane M. Sepa-Kishi Luis F. B. Marqueze Franciane T. F. Vasconcellos Renata T. Nesi Talita C. B. Pereira Luiza W. Kist Maurício R. Bogo Rolando B. Ceddia Ricardo A. Pinho |
| author_facet | Pauline S. Effting Anand Thirupathi Alexandre P. Müller Bárbara C. Pereira Diane M. Sepa-Kishi Luis F. B. Marqueze Franciane T. F. Vasconcellos Renata T. Nesi Talita C. B. Pereira Luiza W. Kist Maurício R. Bogo Rolando B. Ceddia Ricardo A. Pinho |
| author_sort | Pauline S. Effting |
| collection | DOAJ |
| description | This study investigates whether ladder climbing (LC), as a model of resistance exercise, can reverse whole-body and skeletal muscle deleterious metabolic and inflammatory effects of high-fat (HF) diet-induced obesity in mice. To accomplish this, Swiss mice were fed for 17 weeks either standard chow (SC) or an HF diet and then randomly assigned to remain sedentary or to undergo 8 weeks of LC training with progressive increases in resistance weight. Prior to beginning the exercise intervention, HF-fed animals displayed a 47% increase in body weight (BW) and impaired ability to clear blood glucose during an insulin tolerance test (ITT) when compared to SC animals. However, 8 weeks of LC significantly reduced BW, adipocyte size, as well as glycemia under fasting and during the ITT in HF-fed rats. LC also increased the phosphorylation of Akt<sub>Ser473</sub> and AMPK<sub>Thr172</sub> and reduced tumor necrosis factor-alpha (TNF-α) and interleukin 1 beta (IL1-β) contents in the quadriceps muscles of HF-fed mice. Additionally, LC reduced the gene expression of inflammatory markers and attenuated HF-diet-induced NADPH oxidase subunit gp91phox in skeletal muscles. LC training was effective in reducing adiposity and the content of inflammatory mediators in skeletal muscle and improved whole-body glycemic control in mice fed an HF diet. |
| first_indexed | 2024-03-10T01:00:43Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-10T01:00:43Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-8037d5b3c149488c9a6dc2c2c27f3e1b2023-11-23T14:34:48ZengMDPI AGNutrients2072-66432022-05-011411217910.3390/nu14112179Resistance Exercise Training Improves Metabolic and Inflammatory Control in Adipose and Muscle Tissues in Mice Fed a High-Fat DietPauline S. Effting0Anand Thirupathi1Alexandre P. Müller2Bárbara C. Pereira3Diane M. Sepa-Kishi4Luis F. B. Marqueze5Franciane T. F. Vasconcellos6Renata T. Nesi7Talita C. B. Pereira8Luiza W. Kist9Maurício R. Bogo10Rolando B. Ceddia11Ricardo A. Pinho12Faculty of Sports Science, Ningbo University, Ningbo 315211, ChinaFaculty of Sports Science, Ningbo University, Ningbo 315211, ChinaGraduate de Pós-graduação em Farmacologia, Universidade Federal de Santa Catarina, Florianópolis 88020-302, SC, BrazilGraduate Program in Health Science, Medical School, Universidade do Extremo Sul Catarinense, Criciúma 88806-000, SC, BrazilHealth Research Centre, School of Kinesiology and Health Science, York University, Toronto, ON M3J 1P3, CanadaLaboratory of Exercise Biochemistry in Health, Graduate Program in Health Sciences, School of Medicine, Pontifícia Universidade Católica do Paraná, Curitiba 80215-901, PR, BrazilLaboratory of Exercise Biochemistry in Health, Graduate Program in Health Sciences, School of Medicine, Pontifícia Universidade Católica do Paraná, Curitiba 80215-901, PR, BrazilLaboratory of Exercise Biochemistry in Health, Graduate Program in Health Sciences, School of Medicine, Pontifícia Universidade Católica do Paraná, Curitiba 80215-901, PR, BrazilGraduate Program in Cellular and Molecular Biology, School of Health and Life Sciences, Pontifical Catholic University of Rio Grande do Sul, Porto Alegre 90619-900, RS, BrazilGraduate Program in Cellular and Molecular Biology, School of Health and Life Sciences, Pontifical Catholic University of Rio Grande do Sul, Porto Alegre 90619-900, RS, BrazilGraduate Program in Cellular and Molecular Biology, School of Health and Life Sciences, Pontifical Catholic University of Rio Grande do Sul, Porto Alegre 90619-900, RS, BrazilHealth Research Centre, School of Kinesiology and Health Science, York University, Toronto, ON M3J 1P3, CanadaFaculty of Sports Science, Ningbo University, Ningbo 315211, ChinaThis study investigates whether ladder climbing (LC), as a model of resistance exercise, can reverse whole-body and skeletal muscle deleterious metabolic and inflammatory effects of high-fat (HF) diet-induced obesity in mice. To accomplish this, Swiss mice were fed for 17 weeks either standard chow (SC) or an HF diet and then randomly assigned to remain sedentary or to undergo 8 weeks of LC training with progressive increases in resistance weight. Prior to beginning the exercise intervention, HF-fed animals displayed a 47% increase in body weight (BW) and impaired ability to clear blood glucose during an insulin tolerance test (ITT) when compared to SC animals. However, 8 weeks of LC significantly reduced BW, adipocyte size, as well as glycemia under fasting and during the ITT in HF-fed rats. LC also increased the phosphorylation of Akt<sub>Ser473</sub> and AMPK<sub>Thr172</sub> and reduced tumor necrosis factor-alpha (TNF-α) and interleukin 1 beta (IL1-β) contents in the quadriceps muscles of HF-fed mice. Additionally, LC reduced the gene expression of inflammatory markers and attenuated HF-diet-induced NADPH oxidase subunit gp91phox in skeletal muscles. LC training was effective in reducing adiposity and the content of inflammatory mediators in skeletal muscle and improved whole-body glycemic control in mice fed an HF diet.https://www.mdpi.com/2072-6643/14/11/2179physical exerciseladder-climbing traininginflammationinsulin resistanceobesity |
| spellingShingle | Pauline S. Effting Anand Thirupathi Alexandre P. Müller Bárbara C. Pereira Diane M. Sepa-Kishi Luis F. B. Marqueze Franciane T. F. Vasconcellos Renata T. Nesi Talita C. B. Pereira Luiza W. Kist Maurício R. Bogo Rolando B. Ceddia Ricardo A. Pinho Resistance Exercise Training Improves Metabolic and Inflammatory Control in Adipose and Muscle Tissues in Mice Fed a High-Fat Diet Nutrients physical exercise ladder-climbing training inflammation insulin resistance obesity |
| title | Resistance Exercise Training Improves Metabolic and Inflammatory Control in Adipose and Muscle Tissues in Mice Fed a High-Fat Diet |
| title_full | Resistance Exercise Training Improves Metabolic and Inflammatory Control in Adipose and Muscle Tissues in Mice Fed a High-Fat Diet |
| title_fullStr | Resistance Exercise Training Improves Metabolic and Inflammatory Control in Adipose and Muscle Tissues in Mice Fed a High-Fat Diet |
| title_full_unstemmed | Resistance Exercise Training Improves Metabolic and Inflammatory Control in Adipose and Muscle Tissues in Mice Fed a High-Fat Diet |
| title_short | Resistance Exercise Training Improves Metabolic and Inflammatory Control in Adipose and Muscle Tissues in Mice Fed a High-Fat Diet |
| title_sort | resistance exercise training improves metabolic and inflammatory control in adipose and muscle tissues in mice fed a high fat diet |
| topic | physical exercise ladder-climbing training inflammation insulin resistance obesity |
| url | https://www.mdpi.com/2072-6643/14/11/2179 |
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