Analyses of the sucrose synthase gene family in cotton: structure, phylogeny and expression patterns
<p>Abstract</p> <p>Background</p> <p>In plants, sucrose synthase (Sus) is widely considered as a key enzyme involved in sucrose metabolism. Several paralogous genes encoding different isozymes of Sus have been identified and characterized in multiple plant genomes, whil...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2012-06-01
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| Series: | BMC Plant Biology |
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| Online Access: | http://www.biomedcentral.com/1471-2229/12/85 |
| _version_ | 1811315853406240768 |
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| author | Chen Aiqun He Shae Li Feifei Li Zhao Ding Mingquan Liu Qingpo Rong Junkang |
| author_facet | Chen Aiqun He Shae Li Feifei Li Zhao Ding Mingquan Liu Qingpo Rong Junkang |
| author_sort | Chen Aiqun |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>In plants, sucrose synthase (Sus) is widely considered as a key enzyme involved in sucrose metabolism. Several paralogous genes encoding different isozymes of Sus have been identified and characterized in multiple plant genomes, while limited information of Sus genes is available to date for cotton.</p> <p>Results</p> <p>Here, we report the molecular cloning, structural organization, phylogenetic evolution and expression profiles of seven Sus genes (<it>GaSus1</it> to 7) identified from diploid fiber cotton (<it>Gossypium arboreum</it>). Comparisons between cDNA and genomic sequences revealed that the cotton <it>GaSus</it> genes were interrupted by multiple introns. Comparative screening of introns in homologous genes demonstrated that the number and position of Sus introns are highly conserved among Sus genes in cotton and other more distantly related plant species. Phylogenetic analysis showed that <it>GaSus1</it>, <it>GaSus2</it>, <it>GaSus3</it>, <it>GaSus4</it> and <it>GaSus5</it> could be clustered together into a dicot Sus group, while <it>GaSus6</it> and <it>GaSus7</it> were separated evenly into other two groups, with members from both dicot and monocot species. Expression profiles analyses of the seven Sus genes indicated that except <it>GaSus2</it>, of which the transcripts was undetectable in all tissues examined, and <it>GaSus7</it>, which was only expressed in stem and petal, the other five paralogues were differentially expressed in a wide ranges of tissues, and showed development-dependent expression profiles in cotton fiber cells.</p> <p>Conclusions</p> <p>This is a comprehensive study of the Sus gene family in cotton plant. The results presented in this work provide new insights into the evolutionary conservation and sub-functional divergence of the cotton Sus gene family in response to cotton fiber growth and development.</p> |
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| id | doaj.art-803a9591c88b40b19d00986f3a685cac |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-04-13T11:38:35Z |
| publishDate | 2012-06-01 |
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| series | BMC Plant Biology |
| spelling | doaj.art-803a9591c88b40b19d00986f3a685cac2022-12-22T02:48:22ZengBMCBMC Plant Biology1471-22292012-06-011218510.1186/1471-2229-12-85Analyses of the sucrose synthase gene family in cotton: structure, phylogeny and expression patternsChen AiqunHe ShaeLi FeifeiLi ZhaoDing MingquanLiu QingpoRong Junkang<p>Abstract</p> <p>Background</p> <p>In plants, sucrose synthase (Sus) is widely considered as a key enzyme involved in sucrose metabolism. Several paralogous genes encoding different isozymes of Sus have been identified and characterized in multiple plant genomes, while limited information of Sus genes is available to date for cotton.</p> <p>Results</p> <p>Here, we report the molecular cloning, structural organization, phylogenetic evolution and expression profiles of seven Sus genes (<it>GaSus1</it> to 7) identified from diploid fiber cotton (<it>Gossypium arboreum</it>). Comparisons between cDNA and genomic sequences revealed that the cotton <it>GaSus</it> genes were interrupted by multiple introns. Comparative screening of introns in homologous genes demonstrated that the number and position of Sus introns are highly conserved among Sus genes in cotton and other more distantly related plant species. Phylogenetic analysis showed that <it>GaSus1</it>, <it>GaSus2</it>, <it>GaSus3</it>, <it>GaSus4</it> and <it>GaSus5</it> could be clustered together into a dicot Sus group, while <it>GaSus6</it> and <it>GaSus7</it> were separated evenly into other two groups, with members from both dicot and monocot species. Expression profiles analyses of the seven Sus genes indicated that except <it>GaSus2</it>, of which the transcripts was undetectable in all tissues examined, and <it>GaSus7</it>, which was only expressed in stem and petal, the other five paralogues were differentially expressed in a wide ranges of tissues, and showed development-dependent expression profiles in cotton fiber cells.</p> <p>Conclusions</p> <p>This is a comprehensive study of the Sus gene family in cotton plant. The results presented in this work provide new insights into the evolutionary conservation and sub-functional divergence of the cotton Sus gene family in response to cotton fiber growth and development.</p>http://www.biomedcentral.com/1471-2229/12/85Sucrose synthaseGene structurePhylogenyExpression patternCotton |
| spellingShingle | Chen Aiqun He Shae Li Feifei Li Zhao Ding Mingquan Liu Qingpo Rong Junkang Analyses of the sucrose synthase gene family in cotton: structure, phylogeny and expression patterns BMC Plant Biology Sucrose synthase Gene structure Phylogeny Expression pattern Cotton |
| title | Analyses of the sucrose synthase gene family in cotton: structure, phylogeny and expression patterns |
| title_full | Analyses of the sucrose synthase gene family in cotton: structure, phylogeny and expression patterns |
| title_fullStr | Analyses of the sucrose synthase gene family in cotton: structure, phylogeny and expression patterns |
| title_full_unstemmed | Analyses of the sucrose synthase gene family in cotton: structure, phylogeny and expression patterns |
| title_short | Analyses of the sucrose synthase gene family in cotton: structure, phylogeny and expression patterns |
| title_sort | analyses of the sucrose synthase gene family in cotton structure phylogeny and expression patterns |
| topic | Sucrose synthase Gene structure Phylogeny Expression pattern Cotton |
| url | http://www.biomedcentral.com/1471-2229/12/85 |
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