Analysis of a cellular structure observed in the compound eyes of Drosophila white; yata mutants and white mutants
We previously identified the Drosophila yata mutant, which showed phenotypes including progressive vacuolization of the white-coloured compound eye, progressive shrinkage of the brain and a shortened lifespan. The yata gene was shown to be involved in controlling intracellular trafficking of the Amy...
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The Company of Biologists
2020-01-01
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| Series: | Biology Open |
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| Online Access: | http://bio.biologists.org/content/9/1/bio047043 |
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| author | Eri Arimoto Yutaro Kawashima Taein Choi Mami Unagami Shintaro Akiyama Mizuki Tomizawa Hiroyuki Yano Emiko Suzuki Masaki Sone |
| author_facet | Eri Arimoto Yutaro Kawashima Taein Choi Mami Unagami Shintaro Akiyama Mizuki Tomizawa Hiroyuki Yano Emiko Suzuki Masaki Sone |
| author_sort | Eri Arimoto |
| collection | DOAJ |
| description | We previously identified the Drosophila yata mutant, which showed phenotypes including progressive vacuolization of the white-coloured compound eye, progressive shrinkage of the brain and a shortened lifespan. The yata gene was shown to be involved in controlling intracellular trafficking of the Amyloid precursor protein-like protein, which is an orthologue of Amyloid precursor protein, which is a causative molecule of Alzheimer's disease. In this study, we examined the phenotype of the compound eye of the yata mutant using electron microscopy and confocal microscopy. We found that abnormal cellular structures that seemed to originate from bleb-like structures and contained vesicles and organelles, such as multivesicular bodies and autophagosomes, were observed in aged white; yata mutants and aged white mutants. These structures were not observed in newly eclosed flies and the presence of the structures was suppressed in flies grown under constant dark conditions after eclosion. The structures were not observed in newly eclosed red-eyed yata mutants or wild-type flies, but were observed in very aged red-eyed wild-type flies. Thus, our data suggest that the observed structures are formed as a result of changes associated with exposure to light after eclosion in white mutants, white; yata mutants and aged flies. |
| first_indexed | 2024-12-17T00:53:14Z |
| format | Article |
| id | doaj.art-803b1dc45a06401492861a934ee209de |
| institution | Directory Open Access Journal |
| issn | 2046-6390 |
| language | English |
| last_indexed | 2024-12-17T00:53:14Z |
| publishDate | 2020-01-01 |
| publisher | The Company of Biologists |
| record_format | Article |
| series | Biology Open |
| spelling | doaj.art-803b1dc45a06401492861a934ee209de2022-12-21T22:09:42ZengThe Company of BiologistsBiology Open2046-63902020-01-019110.1242/bio.047043047043Analysis of a cellular structure observed in the compound eyes of Drosophila white; yata mutants and white mutantsEri Arimoto0Yutaro Kawashima1Taein Choi2Mami Unagami3Shintaro Akiyama4Mizuki Tomizawa5Hiroyuki Yano6Emiko Suzuki7Masaki Sone8 Department of Biomolecular Science, Faculty of Science, Toho University, Funabashi 274-8510, Japan Department of Biomolecular Science, Faculty of Science, Toho University, Funabashi 274-8510, Japan Department of Biomolecular Science, Faculty of Science, Toho University, Funabashi 274-8510, Japan Department of Biomolecular Science, Faculty of Science, Toho University, Funabashi 274-8510, Japan Department of Biomolecular Science, Faculty of Science, Toho University, Funabashi 274-8510, Japan Department of Biomolecular Science, Faculty of Science, Toho University, Funabashi 274-8510, Japan Technical Section, National Institute of Genetics, Mishima 411-8540, Japan Department of Gene Function and Phenomics, National Institute of Genetics, Mishima 411-8540, Japan Department of Biomolecular Science, Faculty of Science, Toho University, Funabashi 274-8510, Japan We previously identified the Drosophila yata mutant, which showed phenotypes including progressive vacuolization of the white-coloured compound eye, progressive shrinkage of the brain and a shortened lifespan. The yata gene was shown to be involved in controlling intracellular trafficking of the Amyloid precursor protein-like protein, which is an orthologue of Amyloid precursor protein, which is a causative molecule of Alzheimer's disease. In this study, we examined the phenotype of the compound eye of the yata mutant using electron microscopy and confocal microscopy. We found that abnormal cellular structures that seemed to originate from bleb-like structures and contained vesicles and organelles, such as multivesicular bodies and autophagosomes, were observed in aged white; yata mutants and aged white mutants. These structures were not observed in newly eclosed flies and the presence of the structures was suppressed in flies grown under constant dark conditions after eclosion. The structures were not observed in newly eclosed red-eyed yata mutants or wild-type flies, but were observed in very aged red-eyed wild-type flies. Thus, our data suggest that the observed structures are formed as a result of changes associated with exposure to light after eclosion in white mutants, white; yata mutants and aged flies.http://bio.biologists.org/content/9/1/bio047043drosophilaeyephotoreceptorblebageingautophagy |
| spellingShingle | Eri Arimoto Yutaro Kawashima Taein Choi Mami Unagami Shintaro Akiyama Mizuki Tomizawa Hiroyuki Yano Emiko Suzuki Masaki Sone Analysis of a cellular structure observed in the compound eyes of Drosophila white; yata mutants and white mutants Biology Open drosophila eye photoreceptor bleb ageing autophagy |
| title | Analysis of a cellular structure observed in the compound eyes of Drosophila white; yata mutants and white mutants |
| title_full | Analysis of a cellular structure observed in the compound eyes of Drosophila white; yata mutants and white mutants |
| title_fullStr | Analysis of a cellular structure observed in the compound eyes of Drosophila white; yata mutants and white mutants |
| title_full_unstemmed | Analysis of a cellular structure observed in the compound eyes of Drosophila white; yata mutants and white mutants |
| title_short | Analysis of a cellular structure observed in the compound eyes of Drosophila white; yata mutants and white mutants |
| title_sort | analysis of a cellular structure observed in the compound eyes of drosophila white yata mutants and white mutants |
| topic | drosophila eye photoreceptor bleb ageing autophagy |
| url | http://bio.biologists.org/content/9/1/bio047043 |
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