Single-cell RNA sequencing reveals the role of immune-related autophagy in spinal cord injury in rats
Spinal cord injury refers to damage to the spinal cord due to trauma, disease, or degeneration; and the number of new cases is increasing yearly. Significant cellular changes are known to occur in the area of spinal cord injury. However, changes in cellular composition, trajectory of cell developmen...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2022.987344/full |
| _version_ | 1818060841714450432 |
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| author | Erliang Li Rongbao Yan Kang Yan Rui Zhang Qian Zhang Peng Zou Huimei Wang Huan Qiao Shuang Li Qiong Ma Bo Liao |
| author_facet | Erliang Li Rongbao Yan Kang Yan Rui Zhang Qian Zhang Peng Zou Huimei Wang Huan Qiao Shuang Li Qiong Ma Bo Liao |
| author_sort | Erliang Li |
| collection | DOAJ |
| description | Spinal cord injury refers to damage to the spinal cord due to trauma, disease, or degeneration; and the number of new cases is increasing yearly. Significant cellular changes are known to occur in the area of spinal cord injury. However, changes in cellular composition, trajectory of cell development, and intercellular communication in the injured area remain unclear. Here, we used single-cell RNA sequencing to evaluate almost all the cell types that constitute the site of spinal cord injury in rats. In addition to mapping the cells of the injured area, we screened the expression of immune autophagy-related factors in cells and identified signaling pathways by the measuring the expression of the receptor−ligand pairs to regulate specific cell interactions during autophagy after spinal cord injury. Our data set is a valuable resource that provides new insights into the pathobiology of spinal cord injury and other traumatic diseases of the central nervous system. |
| first_indexed | 2024-12-10T13:38:50Z |
| format | Article |
| id | doaj.art-803c59d071a744afa02e9673b90d346c |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-10T13:38:50Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-803c59d071a744afa02e9673b90d346c2022-12-22T01:46:46ZengFrontiers Media S.A.Frontiers in Immunology1664-32242022-09-011310.3389/fimmu.2022.987344987344Single-cell RNA sequencing reveals the role of immune-related autophagy in spinal cord injury in ratsErliang Li0Rongbao Yan1Kang Yan2Rui Zhang3Qian Zhang4Peng Zou5Huimei Wang6Huan Qiao7Shuang Li8Qiong Ma9Bo Liao10Department of Orthopaedics, The Second Affiliated Hospital of Air Force Military Medical University, Xi’an, ChinaDepartment of Orthopaedics, The First Affiliated Hospital of Harbin Medical University, Harbin, ChinaDepartment of Orthopaedics, The Second Affiliated Hospital of Air Force Military Medical University, Xi’an, ChinaDepartment of Orthopaedics, The Second Affiliated Hospital of Air Force Military Medical University, Xi’an, ChinaDepartment of Orthopaedics, The Second Affiliated Hospital of Air Force Military Medical University, Xi’an, ChinaDepartment of Orthopaedics, The Second Affiliated Hospital of Air Force Military Medical University, Xi’an, ChinaDepartment of Pathology, Zhongshan Hospital, Fudan University, Shanghai, ChinaDepartment of Orthopaedics, The Second Affiliated Hospital of Air Force Military Medical University, Xi’an, ChinaDepartment of Orthopaedics, The Second Affiliated Hospital of Air Force Military Medical University, Xi’an, ChinaDepartment of Orthopaedics, The Second Affiliated Hospital of Air Force Military Medical University, Xi’an, ChinaDepartment of Orthopaedics, The Second Affiliated Hospital of Air Force Military Medical University, Xi’an, ChinaSpinal cord injury refers to damage to the spinal cord due to trauma, disease, or degeneration; and the number of new cases is increasing yearly. Significant cellular changes are known to occur in the area of spinal cord injury. However, changes in cellular composition, trajectory of cell development, and intercellular communication in the injured area remain unclear. Here, we used single-cell RNA sequencing to evaluate almost all the cell types that constitute the site of spinal cord injury in rats. In addition to mapping the cells of the injured area, we screened the expression of immune autophagy-related factors in cells and identified signaling pathways by the measuring the expression of the receptor−ligand pairs to regulate specific cell interactions during autophagy after spinal cord injury. Our data set is a valuable resource that provides new insights into the pathobiology of spinal cord injury and other traumatic diseases of the central nervous system.https://www.frontiersin.org/articles/10.3389/fimmu.2022.987344/fullsingle-cell RNA sequencingimmuneautophagyspinal cord injurymicroglia |
| spellingShingle | Erliang Li Rongbao Yan Kang Yan Rui Zhang Qian Zhang Peng Zou Huimei Wang Huan Qiao Shuang Li Qiong Ma Bo Liao Single-cell RNA sequencing reveals the role of immune-related autophagy in spinal cord injury in rats Frontiers in Immunology single-cell RNA sequencing immune autophagy spinal cord injury microglia |
| title | Single-cell RNA sequencing reveals the role of immune-related autophagy in spinal cord injury in rats |
| title_full | Single-cell RNA sequencing reveals the role of immune-related autophagy in spinal cord injury in rats |
| title_fullStr | Single-cell RNA sequencing reveals the role of immune-related autophagy in spinal cord injury in rats |
| title_full_unstemmed | Single-cell RNA sequencing reveals the role of immune-related autophagy in spinal cord injury in rats |
| title_short | Single-cell RNA sequencing reveals the role of immune-related autophagy in spinal cord injury in rats |
| title_sort | single cell rna sequencing reveals the role of immune related autophagy in spinal cord injury in rats |
| topic | single-cell RNA sequencing immune autophagy spinal cord injury microglia |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2022.987344/full |
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