Generation and Characterization of Immortalized Mouse Cortical Astrocytes From Wildtype and Connexin43 Knockout Mice

Generation and Characterization of Immortalized Mouse Cortical Astrocytes From Wildtype and Connexin43 Knockout Mice

We transduced mouse cortical astrocytes cultured from four litters of embryonic wildtype (WT) and connexin43 (Cx43) null mouse pups with lentiviral vector encoding hTERT and measured expression of astrocyte-specific markers up to passage 10 (p10). The immortalized cell lines thus generated (designat...

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Bibliographic Details
Main Authors: Antonio Cibelli, Sandra Veronica Lopez-Quintero, Sean Mccutcheon, Eliana Scemes, David C. Spray, Randy F. Stout, Sylvia O. Suadicani, Mia M. Thi, Marcia Urban-Maldonado
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-03-01
Series:Frontiers in Cellular Neuroscience
Subjects:
hTERT
Cx43 (connexin43)
gap junction
cell model
astrocytes
immortalized astrocytes
Online Access:https://www.frontiersin.org/articles/10.3389/fncel.2021.647109/full

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