Developing functional markers for vitamin E biosynthesis in oil palm
Vitamin E is essential for human health and plays positive roles in anti-oxidation. Previously, we detected large variation in vitamin E content among 161 oil palm accessions. In this study, twenty oil palm accessions with distinct variation in vitamin E contents (171.30 to 1 258.50 ppm) were select...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2021-01-01
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| Series: | PLoS ONE |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8604351/?tool=EBI |
| _version_ | 1830283784609595392 |
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| author | Yajing Dou Wei Xia Annaliese S. Mason Dongyi Huang Xiwei Sun Haikuo Fan Yong Xiao |
| author_facet | Yajing Dou Wei Xia Annaliese S. Mason Dongyi Huang Xiwei Sun Haikuo Fan Yong Xiao |
| author_sort | Yajing Dou |
| collection | DOAJ |
| description | Vitamin E is essential for human health and plays positive roles in anti-oxidation. Previously, we detected large variation in vitamin E content among 161 oil palm accessions. In this study, twenty oil palm accessions with distinct variation in vitamin E contents (171.30 to 1 258.50 ppm) were selected for genetic variation analysis and developing functional markers associated with vitamin E contents. Thirty-seven homologous genes in oil palm belonging to vitamin E biosynthesis pathway were identified via BLASTP analysis, the lengths of which ranged from 426 to 25 717 bp (average 7 089 bp). Multiplex PCR sequencing for the 37 genes found 1 703 SNPs and 85 indels among the 20 oil palm accessions, with 226 SNPs locating in the coding regions. Clustering analysis for these polymorphic loci showed that the 20 oil palm accessions could be divided into five groups. Among these groups, group I included eight oil palm accessions whose vitamin E content (mean value: 893.50 ppm) was far higher than other groups (mean value 256.29 to 532.94 ppm). Correlation analysis between the markers and vitamin E traits showed that 134 SNP and 7 indel markers were significantly (p < 0.05) related with total vitamin E content. Among these functional markers, the indel EgTMT-1-24 was highly correlated with variation in vitamin E content, especially tocotrienol content. Our study identified a number of candidate function associated markers and provided clues for further research into molecular breeding for high vitamin E content oil palm. |
| first_indexed | 2024-12-19T03:06:17Z |
| format | Article |
| id | doaj.art-804178369da04d2c86b463153dca61cf |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-19T03:06:17Z |
| publishDate | 2021-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-804178369da04d2c86b463153dca61cf2022-12-21T20:38:06ZengPublic Library of Science (PLoS)PLoS ONE1932-62032021-01-011611Developing functional markers for vitamin E biosynthesis in oil palmYajing DouWei XiaAnnaliese S. MasonDongyi HuangXiwei SunHaikuo FanYong XiaoVitamin E is essential for human health and plays positive roles in anti-oxidation. Previously, we detected large variation in vitamin E content among 161 oil palm accessions. In this study, twenty oil palm accessions with distinct variation in vitamin E contents (171.30 to 1 258.50 ppm) were selected for genetic variation analysis and developing functional markers associated with vitamin E contents. Thirty-seven homologous genes in oil palm belonging to vitamin E biosynthesis pathway were identified via BLASTP analysis, the lengths of which ranged from 426 to 25 717 bp (average 7 089 bp). Multiplex PCR sequencing for the 37 genes found 1 703 SNPs and 85 indels among the 20 oil palm accessions, with 226 SNPs locating in the coding regions. Clustering analysis for these polymorphic loci showed that the 20 oil palm accessions could be divided into five groups. Among these groups, group I included eight oil palm accessions whose vitamin E content (mean value: 893.50 ppm) was far higher than other groups (mean value 256.29 to 532.94 ppm). Correlation analysis between the markers and vitamin E traits showed that 134 SNP and 7 indel markers were significantly (p < 0.05) related with total vitamin E content. Among these functional markers, the indel EgTMT-1-24 was highly correlated with variation in vitamin E content, especially tocotrienol content. Our study identified a number of candidate function associated markers and provided clues for further research into molecular breeding for high vitamin E content oil palm.https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8604351/?tool=EBI |
| spellingShingle | Yajing Dou Wei Xia Annaliese S. Mason Dongyi Huang Xiwei Sun Haikuo Fan Yong Xiao Developing functional markers for vitamin E biosynthesis in oil palm PLoS ONE |
| title | Developing functional markers for vitamin E biosynthesis in oil palm |
| title_full | Developing functional markers for vitamin E biosynthesis in oil palm |
| title_fullStr | Developing functional markers for vitamin E biosynthesis in oil palm |
| title_full_unstemmed | Developing functional markers for vitamin E biosynthesis in oil palm |
| title_short | Developing functional markers for vitamin E biosynthesis in oil palm |
| title_sort | developing functional markers for vitamin e biosynthesis in oil palm |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8604351/?tool=EBI |
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