Genetic Analysis of Integrons among Methicillin-resistant and Susceptible Staphylococcus aureus Isolated from Nosocomial Infections
Abstract Background: Integrons are mobile genetic elements that play an important role in dissemination of antibiotic resistance genes. The aim of present study is to determine the antibiotic resistance profile, frequency of integrons genes (class 1, 2, 3) and compare it between MRSA and MSSA isol...
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| Format: | Article |
| Language: | fas |
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Arak Medical University
2017-10-01
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| Series: | Majallah-i dānishgāh-i ̒ulūm-i pizishkī-i Arāk |
| Subjects: | |
| Online Access: | http://amuj.arakmu.ac.ir/article-1-5235-en.pdf |
| _version_ | 1817994128518021120 |
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| author | Somayyeh Moatti Behrouz Shojaee Sadi Ehsanollah Ghaznavi-rad |
| author_facet | Somayyeh Moatti Behrouz Shojaee Sadi Ehsanollah Ghaznavi-rad |
| author_sort | Somayyeh Moatti |
| collection | DOAJ |
| description | Abstract
Background: Integrons are mobile genetic elements that play an important role in dissemination of antibiotic resistance genes. The aim of present study is to determine the antibiotic resistance profile, frequency of integrons genes (class 1, 2, 3) and compare it between MRSA and MSSA isolates from clinical infections.
Materials and Methods: 50 MRSA and 50 MSSA isolates from March to September 2015 were isolated from infection site of hospitalized patients referred to Valiasr hospital Arak, Iran were subjected to this study. All isolates were tested for susceptibility to antibiotics using disk diffusion method. Then, the mecA gene was studied to validate resistance. The frequency of integrons (class 1, 2, 3) and the variable region genes like qacE 1 and sul1 in isolates were determined by PCR method.
Results: The highest antibiotic resistances rate in isolates was found for clindamycin.
All of the isolates were susceptibel to vancomycin. 80% of MRSA and 40% of the MSSA isolates carried class 1 integrons, whereas class 2 integron were found in 12 % and 4% of MRSA and MSSA isolates, respectively. Also, all isolates that were class 1 integron gene positive contain qacE 1 and sul1 genes. Class 3 integrons were not found.
Conclusion: The high frequency of class 1 integron in MRSA and MSSA isolates associated with high rate of antibiotic resistance indicating that may be integrons play an important role facilitating the spread of antimicrobial resistance in this region. Clinical doctors and infection control committee should take this issue seriously. |
| first_indexed | 2024-04-14T01:48:54Z |
| format | Article |
| id | doaj.art-80470d3413714eb3ac2e9155a2a5a07f |
| institution | Directory Open Access Journal |
| issn | 1735-5338 2008-644X |
| language | fas |
| last_indexed | 2024-04-14T01:48:54Z |
| publishDate | 2017-10-01 |
| publisher | Arak Medical University |
| record_format | Article |
| series | Majallah-i dānishgāh-i ̒ulūm-i pizishkī-i Arāk |
| spelling | doaj.art-80470d3413714eb3ac2e9155a2a5a07f2022-12-22T02:19:26ZfasArak Medical UniversityMajallah-i dānishgāh-i ̒ulūm-i pizishkī-i Arāk1735-53382008-644X2017-10-0120798107Genetic Analysis of Integrons among Methicillin-resistant and Susceptible Staphylococcus aureus Isolated from Nosocomial InfectionsSomayyeh Moatti0Behrouz Shojaee Sadi1Ehsanollah Ghaznavi-rad2MSc in Microbiology, Arak Branch, Islamic Azad University, Arak, IranInstructor, Department of Microbiology, Arak Branch, Islamic Azad University, Arak, IranAssociate Professor, Department of Medical Microbiology and Immunology, Molecular Medicine Research Center, Faculty of Medicine, Arak University of Medical Sciences, Arak, IranAbstract Background: Integrons are mobile genetic elements that play an important role in dissemination of antibiotic resistance genes. The aim of present study is to determine the antibiotic resistance profile, frequency of integrons genes (class 1, 2, 3) and compare it between MRSA and MSSA isolates from clinical infections. Materials and Methods: 50 MRSA and 50 MSSA isolates from March to September 2015 were isolated from infection site of hospitalized patients referred to Valiasr hospital Arak, Iran were subjected to this study. All isolates were tested for susceptibility to antibiotics using disk diffusion method. Then, the mecA gene was studied to validate resistance. The frequency of integrons (class 1, 2, 3) and the variable region genes like qacE 1 and sul1 in isolates were determined by PCR method. Results: The highest antibiotic resistances rate in isolates was found for clindamycin. All of the isolates were susceptibel to vancomycin. 80% of MRSA and 40% of the MSSA isolates carried class 1 integrons, whereas class 2 integron were found in 12 % and 4% of MRSA and MSSA isolates, respectively. Also, all isolates that were class 1 integron gene positive contain qacE 1 and sul1 genes. Class 3 integrons were not found. Conclusion: The high frequency of class 1 integron in MRSA and MSSA isolates associated with high rate of antibiotic resistance indicating that may be integrons play an important role facilitating the spread of antimicrobial resistance in this region. Clinical doctors and infection control committee should take this issue seriously.http://amuj.arakmu.ac.ir/article-1-5235-en.pdfIntegronMethicillin-resistant Staphylococcus aureusMethicillin-susceptible Staphylococcus aureusPolymerase chain reaction |
| spellingShingle | Somayyeh Moatti Behrouz Shojaee Sadi Ehsanollah Ghaznavi-rad Genetic Analysis of Integrons among Methicillin-resistant and Susceptible Staphylococcus aureus Isolated from Nosocomial Infections Majallah-i dānishgāh-i ̒ulūm-i pizishkī-i Arāk Integron Methicillin-resistant Staphylococcus aureus Methicillin-susceptible Staphylococcus aureus Polymerase chain reaction |
| title | Genetic Analysis of Integrons among Methicillin-resistant and Susceptible Staphylococcus aureus Isolated from Nosocomial Infections |
| title_full | Genetic Analysis of Integrons among Methicillin-resistant and Susceptible Staphylococcus aureus Isolated from Nosocomial Infections |
| title_fullStr | Genetic Analysis of Integrons among Methicillin-resistant and Susceptible Staphylococcus aureus Isolated from Nosocomial Infections |
| title_full_unstemmed | Genetic Analysis of Integrons among Methicillin-resistant and Susceptible Staphylococcus aureus Isolated from Nosocomial Infections |
| title_short | Genetic Analysis of Integrons among Methicillin-resistant and Susceptible Staphylococcus aureus Isolated from Nosocomial Infections |
| title_sort | genetic analysis of integrons among methicillin resistant and susceptible staphylococcus aureus isolated from nosocomial infections |
| topic | Integron Methicillin-resistant Staphylococcus aureus Methicillin-susceptible Staphylococcus aureus Polymerase chain reaction |
| url | http://amuj.arakmu.ac.ir/article-1-5235-en.pdf |
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