<it>Staphylococcus aureus </it>sigma B-dependent emergence of small-colony variants and biofilm production following exposure to <it>Pseudomonas aeruginosa </it>4-hydroxy-2-heptylquinoline-<it>N-</it>oxide
<p>Abstract</p> <p>Background</p> <p><it>Staphylococcus aureus </it>and <it>Pseudomonas aeruginosa </it>are often found together in the airways of cystic fibrosis (CF) patients. It was previously shown that the <it>P. aeruginosa </it>...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2010-01-01
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| Series: | BMC Microbiology |
| Online Access: | http://www.biomedcentral.com/1471-2180/10/33 |
| _version_ | 1811250671175860224 |
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| author | Michaud Sophie Frost Eric H Cantin André M Déziel Eric Asselin Ann-Elise Séguin David Mitchell Gabriel Malouin François |
| author_facet | Michaud Sophie Frost Eric H Cantin André M Déziel Eric Asselin Ann-Elise Séguin David Mitchell Gabriel Malouin François |
| author_sort | Michaud Sophie |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p><it>Staphylococcus aureus </it>and <it>Pseudomonas aeruginosa </it>are often found together in the airways of cystic fibrosis (CF) patients. It was previously shown that the <it>P. aeruginosa </it>exoproduct 4-hydroxy-2-heptylquinoline-<it>N-</it>oxide (HQNO) suppresses the growth of <it>S. aureus </it>and provokes the emergence of small-colony variants (SCVs). The presence of <it>S. aureus </it>SCVs as well as biofilms have both been associated with chronic infections in CF.</p> <p>Results</p> <p>We demonstrated that HQNO stimulates <it>S. aureus </it>to form a biofilm in association with the formation of SCVs. The emergence of SCVs and biofilm production under HQNO exposure was shown to be dependent on the activity of the stress- and colonization-related alternative sigma factor B (SigB). Analysis of gene expression revealed that exposure of a prototypical <it>S. aureus </it>strain to HQNO activates SigB, which was leading to an increase in the expression of the fibronectin-binding protein A and the biofilm-associated <it>sarA </it>genes. Conversely, the quorum sensing accessory gene regulator (<it>agr</it>) system and the α-hemolysin gene were repressed by HQNO. Experiments using culture supernatants from <it>P. aeruginosa </it>PAO1 and a double chamber co-culture model confirmed that <it>P. aeruginosa </it>stimulates biofilm formation and activates SigB in a <it>S. aureus </it>strain isolated from a CF patient. Furthermore, the supernatant from <it>P. aeruginosa </it>mutants unable to produce HQNO induced the production of biofilms by <it>S. aureus </it>to a lesser extent than the wild-type strain only in a <it>S. aureus </it>SigB-functional background.</p> <p>Conclusions</p> <p>These results suggest that <it>S. aureus </it>responds to HQNO from <it>P. aeruginosa </it>by forming SCVs and biofilms through SigB activation, a phenomenon that may contribute to the establishment of chronic infections in CF patients.</p> |
| first_indexed | 2024-04-12T16:08:09Z |
| format | Article |
| id | doaj.art-804746b4ddc24c40b56d184551a32d08 |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-04-12T16:08:09Z |
| publishDate | 2010-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-804746b4ddc24c40b56d184551a32d082022-12-22T03:25:59ZengBMCBMC Microbiology1471-21802010-01-011013310.1186/1471-2180-10-33<it>Staphylococcus aureus </it>sigma B-dependent emergence of small-colony variants and biofilm production following exposure to <it>Pseudomonas aeruginosa </it>4-hydroxy-2-heptylquinoline-<it>N-</it>oxideMichaud SophieFrost Eric HCantin André MDéziel EricAsselin Ann-EliseSéguin DavidMitchell GabrielMalouin François<p>Abstract</p> <p>Background</p> <p><it>Staphylococcus aureus </it>and <it>Pseudomonas aeruginosa </it>are often found together in the airways of cystic fibrosis (CF) patients. It was previously shown that the <it>P. aeruginosa </it>exoproduct 4-hydroxy-2-heptylquinoline-<it>N-</it>oxide (HQNO) suppresses the growth of <it>S. aureus </it>and provokes the emergence of small-colony variants (SCVs). The presence of <it>S. aureus </it>SCVs as well as biofilms have both been associated with chronic infections in CF.</p> <p>Results</p> <p>We demonstrated that HQNO stimulates <it>S. aureus </it>to form a biofilm in association with the formation of SCVs. The emergence of SCVs and biofilm production under HQNO exposure was shown to be dependent on the activity of the stress- and colonization-related alternative sigma factor B (SigB). Analysis of gene expression revealed that exposure of a prototypical <it>S. aureus </it>strain to HQNO activates SigB, which was leading to an increase in the expression of the fibronectin-binding protein A and the biofilm-associated <it>sarA </it>genes. Conversely, the quorum sensing accessory gene regulator (<it>agr</it>) system and the α-hemolysin gene were repressed by HQNO. Experiments using culture supernatants from <it>P. aeruginosa </it>PAO1 and a double chamber co-culture model confirmed that <it>P. aeruginosa </it>stimulates biofilm formation and activates SigB in a <it>S. aureus </it>strain isolated from a CF patient. Furthermore, the supernatant from <it>P. aeruginosa </it>mutants unable to produce HQNO induced the production of biofilms by <it>S. aureus </it>to a lesser extent than the wild-type strain only in a <it>S. aureus </it>SigB-functional background.</p> <p>Conclusions</p> <p>These results suggest that <it>S. aureus </it>responds to HQNO from <it>P. aeruginosa </it>by forming SCVs and biofilms through SigB activation, a phenomenon that may contribute to the establishment of chronic infections in CF patients.</p>http://www.biomedcentral.com/1471-2180/10/33 |
| spellingShingle | Michaud Sophie Frost Eric H Cantin André M Déziel Eric Asselin Ann-Elise Séguin David Mitchell Gabriel Malouin François <it>Staphylococcus aureus </it>sigma B-dependent emergence of small-colony variants and biofilm production following exposure to <it>Pseudomonas aeruginosa </it>4-hydroxy-2-heptylquinoline-<it>N-</it>oxide BMC Microbiology |
| title | <it>Staphylococcus aureus </it>sigma B-dependent emergence of small-colony variants and biofilm production following exposure to <it>Pseudomonas aeruginosa </it>4-hydroxy-2-heptylquinoline-<it>N-</it>oxide |
| title_full | <it>Staphylococcus aureus </it>sigma B-dependent emergence of small-colony variants and biofilm production following exposure to <it>Pseudomonas aeruginosa </it>4-hydroxy-2-heptylquinoline-<it>N-</it>oxide |
| title_fullStr | <it>Staphylococcus aureus </it>sigma B-dependent emergence of small-colony variants and biofilm production following exposure to <it>Pseudomonas aeruginosa </it>4-hydroxy-2-heptylquinoline-<it>N-</it>oxide |
| title_full_unstemmed | <it>Staphylococcus aureus </it>sigma B-dependent emergence of small-colony variants and biofilm production following exposure to <it>Pseudomonas aeruginosa </it>4-hydroxy-2-heptylquinoline-<it>N-</it>oxide |
| title_short | <it>Staphylococcus aureus </it>sigma B-dependent emergence of small-colony variants and biofilm production following exposure to <it>Pseudomonas aeruginosa </it>4-hydroxy-2-heptylquinoline-<it>N-</it>oxide |
| title_sort | it staphylococcus aureus it sigma b dependent emergence of small colony variants and biofilm production following exposure to it pseudomonas aeruginosa it 4 hydroxy 2 heptylquinoline it n it oxide |
| url | http://www.biomedcentral.com/1471-2180/10/33 |
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