Identification of the <i>BcLEA</i> Gene Family and Functional Analysis of the <i>BcLEA73</i> Gene in Wucai (<i>Brassica campestris</i> L.)
Late embryogenesis abundant (LEA) proteins are important developmental proteins in the response of plants to abiotic stress. In our previous study, <i>BcLEA73</i> was differentially expressed under low-temperature stress. Herein, we combined bioinformatics analysis, subcellular localizat...
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MDPI AG
2023-02-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/14/2/415 |
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| author | Yueyue Jiang Shengnan Zhang Hongcheng Xu Hong Tian Mengyun Zhang Shidong Zhu Chenggang Wang Jinfeng Hou Guohu Chen Xiaoyan Tang Wenjie Wang Jianqiang Wu Xingxue Huang Jinlong Zhang Lingyun Yuan |
| author_facet | Yueyue Jiang Shengnan Zhang Hongcheng Xu Hong Tian Mengyun Zhang Shidong Zhu Chenggang Wang Jinfeng Hou Guohu Chen Xiaoyan Tang Wenjie Wang Jianqiang Wu Xingxue Huang Jinlong Zhang Lingyun Yuan |
| author_sort | Yueyue Jiang |
| collection | DOAJ |
| description | Late embryogenesis abundant (LEA) proteins are important developmental proteins in the response of plants to abiotic stress. In our previous study, <i>BcLEA73</i> was differentially expressed under low-temperature stress. Herein, we combined bioinformatics analysis, subcellular localization, expression assays, and stress experiments (including salt, drought, and osmotic stress) to identify and analyze the BcLEA gene family. Gene cloning and functional analysis of <i>BcLEA73</i> were performed in tobacco and <i>Arabidopsis.</i> Based on the sequence homology and the available conservative motif, 82 <i>BrLEA</i> gene family members were identified and were divided into eight subfamilies in the genome-wide database of Chinese cabbage. The analysis showed that the <i>BrLEA73</i> gene was located on chromosome A09 and belonged to the LEA_6 subfamily. Quantitative real-time PCR analysis indicated that the <i>BcLEA</i> genes were differentially expressed to varying degrees in the roots, stems, leaves, and petioles of Wucai. The overexpressed <i>BcLEA73</i> transgenic plants exhibited no significant differences in root length and seed germination rates compared to the wild-type (WT) plants under control conditions. Under salt and osmotic stress treatment, the root length and seed germination rates of the <i>BcLEA73-OE</i> strain were significantly greater than those of WT plants. Under salt stress, the total antioxidant capacity (T-AOC) of the <i>BcLEA73-OE</i> lines increased significantly, and the relative conductivity, (REL), hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) content, and superoxide anion (O<sub>2</sub><sup>−</sup>) production rate decreased significantly. Under drought treatment, the survival rate of the <i>BcLEA73-OE</i> lines was significantly higher than that of WT plants. These results showed that the <i>BcLEA73</i> gene of Wucai functions in enhancing the tolerance of plants to salt, drought, and osmotic stress. This study provides a theoretical basis to explore the relevant functions of the <i>BcLEA</i> gene family members of Wucai. |
| first_indexed | 2024-03-11T08:47:01Z |
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| last_indexed | 2024-03-11T08:47:01Z |
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| spelling | doaj.art-80479b45c01f4a5ea2203c0827761ea32023-11-16T20:42:39ZengMDPI AGGenes2073-44252023-02-0114241510.3390/genes14020415Identification of the <i>BcLEA</i> Gene Family and Functional Analysis of the <i>BcLEA73</i> Gene in Wucai (<i>Brassica campestris</i> L.)Yueyue Jiang0Shengnan Zhang1Hongcheng Xu2Hong Tian3Mengyun Zhang4Shidong Zhu5Chenggang Wang6Jinfeng Hou7Guohu Chen8Xiaoyan Tang9Wenjie Wang10Jianqiang Wu11Xingxue Huang12Jinlong Zhang13Lingyun Yuan14Vegetable Genetics and Breeding Laboratory, College of Horticulture, Anhui Agricultural University, Hefei 230036, ChinaVegetable Genetics and Breeding Laboratory, College of Horticulture, Anhui Agricultural University, Hefei 230036, ChinaVegetable Genetics and Breeding Laboratory, College of Horticulture, Anhui Agricultural University, Hefei 230036, ChinaVegetable Genetics and Breeding Laboratory, College of Horticulture, Anhui Agricultural University, Hefei 230036, ChinaVegetable Genetics and Breeding Laboratory, College of Horticulture, Anhui Agricultural University, Hefei 230036, ChinaVegetable Genetics and Breeding Laboratory, College of Horticulture, Anhui Agricultural University, Hefei 230036, ChinaVegetable Genetics and Breeding Laboratory, College of Horticulture, Anhui Agricultural University, Hefei 230036, ChinaVegetable Genetics and Breeding Laboratory, College of Horticulture, Anhui Agricultural University, Hefei 230036, ChinaVegetable Genetics and Breeding Laboratory, College of Horticulture, Anhui Agricultural University, Hefei 230036, ChinaVegetable Genetics and Breeding Laboratory, College of Horticulture, Anhui Agricultural University, Hefei 230036, ChinaVegetable Genetics and Breeding Laboratory, College of Horticulture, Anhui Agricultural University, Hefei 230036, ChinaVegetable Genetics and Breeding Laboratory, College of Horticulture, Anhui Agricultural University, Hefei 230036, ChinaDepartment of Vegetable Culture and Breeding, Wanjiang Vegetable Industrial Technology Institute, Maanshan 238200, ChinaVegetable Industry Office, Agricultural and Rural Bureau of He County, Maanshan 238201, ChinaVegetable Genetics and Breeding Laboratory, College of Horticulture, Anhui Agricultural University, Hefei 230036, ChinaLate embryogenesis abundant (LEA) proteins are important developmental proteins in the response of plants to abiotic stress. In our previous study, <i>BcLEA73</i> was differentially expressed under low-temperature stress. Herein, we combined bioinformatics analysis, subcellular localization, expression assays, and stress experiments (including salt, drought, and osmotic stress) to identify and analyze the BcLEA gene family. Gene cloning and functional analysis of <i>BcLEA73</i> were performed in tobacco and <i>Arabidopsis.</i> Based on the sequence homology and the available conservative motif, 82 <i>BrLEA</i> gene family members were identified and were divided into eight subfamilies in the genome-wide database of Chinese cabbage. The analysis showed that the <i>BrLEA73</i> gene was located on chromosome A09 and belonged to the LEA_6 subfamily. Quantitative real-time PCR analysis indicated that the <i>BcLEA</i> genes were differentially expressed to varying degrees in the roots, stems, leaves, and petioles of Wucai. The overexpressed <i>BcLEA73</i> transgenic plants exhibited no significant differences in root length and seed germination rates compared to the wild-type (WT) plants under control conditions. Under salt and osmotic stress treatment, the root length and seed germination rates of the <i>BcLEA73-OE</i> strain were significantly greater than those of WT plants. Under salt stress, the total antioxidant capacity (T-AOC) of the <i>BcLEA73-OE</i> lines increased significantly, and the relative conductivity, (REL), hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) content, and superoxide anion (O<sub>2</sub><sup>−</sup>) production rate decreased significantly. Under drought treatment, the survival rate of the <i>BcLEA73-OE</i> lines was significantly higher than that of WT plants. These results showed that the <i>BcLEA73</i> gene of Wucai functions in enhancing the tolerance of plants to salt, drought, and osmotic stress. This study provides a theoretical basis to explore the relevant functions of the <i>BcLEA</i> gene family members of Wucai.https://www.mdpi.com/2073-4425/14/2/415bioinformatics analysisfunctional analysisgene cloningLEA proteinWucai |
| spellingShingle | Yueyue Jiang Shengnan Zhang Hongcheng Xu Hong Tian Mengyun Zhang Shidong Zhu Chenggang Wang Jinfeng Hou Guohu Chen Xiaoyan Tang Wenjie Wang Jianqiang Wu Xingxue Huang Jinlong Zhang Lingyun Yuan Identification of the <i>BcLEA</i> Gene Family and Functional Analysis of the <i>BcLEA73</i> Gene in Wucai (<i>Brassica campestris</i> L.) Genes bioinformatics analysis functional analysis gene cloning LEA protein Wucai |
| title | Identification of the <i>BcLEA</i> Gene Family and Functional Analysis of the <i>BcLEA73</i> Gene in Wucai (<i>Brassica campestris</i> L.) |
| title_full | Identification of the <i>BcLEA</i> Gene Family and Functional Analysis of the <i>BcLEA73</i> Gene in Wucai (<i>Brassica campestris</i> L.) |
| title_fullStr | Identification of the <i>BcLEA</i> Gene Family and Functional Analysis of the <i>BcLEA73</i> Gene in Wucai (<i>Brassica campestris</i> L.) |
| title_full_unstemmed | Identification of the <i>BcLEA</i> Gene Family and Functional Analysis of the <i>BcLEA73</i> Gene in Wucai (<i>Brassica campestris</i> L.) |
| title_short | Identification of the <i>BcLEA</i> Gene Family and Functional Analysis of the <i>BcLEA73</i> Gene in Wucai (<i>Brassica campestris</i> L.) |
| title_sort | identification of the i bclea i gene family and functional analysis of the i bclea73 i gene in wucai i brassica campestris i l |
| topic | bioinformatics analysis functional analysis gene cloning LEA protein Wucai |
| url | https://www.mdpi.com/2073-4425/14/2/415 |
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