Highly Sensitive Colorimetric Detection of Ochratoxin A by a Label-Free Aptamer and Gold Nanoparticles
A label-free aptamer-based assay for the highly sensitive and specific detection of Ochratoxin A (OTA) was developed using a cationic polymer and gold nanoparticles (AuNPs). The OTA aptamer was used as a recognition element for the colorimetric detection of OTA based on the aggregation of AuNPs by t...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2015-12-01
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| Series: | Toxins |
| Subjects: | |
| Online Access: | http://www.mdpi.com/2072-6651/7/12/4883 |
| _version_ | 1798002370598141952 |
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| author | Yunxia Luan Jiayi Chen Cheng Li Gang Xie Hailong Fu Zhihong Ma Anxiang Lu |
| author_facet | Yunxia Luan Jiayi Chen Cheng Li Gang Xie Hailong Fu Zhihong Ma Anxiang Lu |
| author_sort | Yunxia Luan |
| collection | DOAJ |
| description | A label-free aptamer-based assay for the highly sensitive and specific detection of Ochratoxin A (OTA) was developed using a cationic polymer and gold nanoparticles (AuNPs). The OTA aptamer was used as a recognition element for the colorimetric detection of OTA based on the aggregation of AuNPs by the cationic polymer. By spectroscopic quantitative analysis, the colorimetric assay could detect OTA down to 0.009 ng/mL with high selectivity in the presence of other interfering toxins. This study offers a new alternative in visual detection methods that is rapid and sensitive for OTA detection. |
| first_indexed | 2024-04-11T11:51:05Z |
| format | Article |
| id | doaj.art-804947e8b29e4c76aae24c3cbcb25151 |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-04-11T11:51:05Z |
| publishDate | 2015-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-804947e8b29e4c76aae24c3cbcb251512022-12-22T04:25:19ZengMDPI AGToxins2072-66512015-12-017125377538510.3390/toxins7124883toxins7124883Highly Sensitive Colorimetric Detection of Ochratoxin A by a Label-Free Aptamer and Gold NanoparticlesYunxia Luan0Jiayi Chen1Cheng Li2Gang Xie3Hailong Fu4Zhihong Ma5Anxiang Lu6Agriculture Environment, Beijing Research Center for Agricultural Standards and Testing, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, ChinaAgriculture Environment, Beijing Research Center for Agricultural Standards and Testing, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, ChinaAgriculture Environment, Beijing Research Center for Agricultural Standards and Testing, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, ChinaGrain Safety, Academy of State Administration of Grain, Beijing 100037, ChinaAgriculture Environment, Beijing Research Center for Agricultural Standards and Testing, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, ChinaAgriculture Environment, Beijing Research Center for Agricultural Standards and Testing, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, ChinaAgriculture Environment, Beijing Research Center for Agricultural Standards and Testing, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, ChinaA label-free aptamer-based assay for the highly sensitive and specific detection of Ochratoxin A (OTA) was developed using a cationic polymer and gold nanoparticles (AuNPs). The OTA aptamer was used as a recognition element for the colorimetric detection of OTA based on the aggregation of AuNPs by the cationic polymer. By spectroscopic quantitative analysis, the colorimetric assay could detect OTA down to 0.009 ng/mL with high selectivity in the presence of other interfering toxins. This study offers a new alternative in visual detection methods that is rapid and sensitive for OTA detection.http://www.mdpi.com/2072-6651/7/12/4883aptamerOchratoxin A (OTA)gold nanoparticlescationic polymercolorimetric assay |
| spellingShingle | Yunxia Luan Jiayi Chen Cheng Li Gang Xie Hailong Fu Zhihong Ma Anxiang Lu Highly Sensitive Colorimetric Detection of Ochratoxin A by a Label-Free Aptamer and Gold Nanoparticles Toxins aptamer Ochratoxin A (OTA) gold nanoparticles cationic polymer colorimetric assay |
| title | Highly Sensitive Colorimetric Detection of Ochratoxin A by a Label-Free Aptamer and Gold Nanoparticles |
| title_full | Highly Sensitive Colorimetric Detection of Ochratoxin A by a Label-Free Aptamer and Gold Nanoparticles |
| title_fullStr | Highly Sensitive Colorimetric Detection of Ochratoxin A by a Label-Free Aptamer and Gold Nanoparticles |
| title_full_unstemmed | Highly Sensitive Colorimetric Detection of Ochratoxin A by a Label-Free Aptamer and Gold Nanoparticles |
| title_short | Highly Sensitive Colorimetric Detection of Ochratoxin A by a Label-Free Aptamer and Gold Nanoparticles |
| title_sort | highly sensitive colorimetric detection of ochratoxin a by a label free aptamer and gold nanoparticles |
| topic | aptamer Ochratoxin A (OTA) gold nanoparticles cationic polymer colorimetric assay |
| url | http://www.mdpi.com/2072-6651/7/12/4883 |
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