Primary, Secondary Metabolites and Molecular Characterization of Hawthorn (<i>Crataegus</i> spp.) Genotypes
In this study, the molecular, biochemical and agro-morphological characterization of genotypes belonging to hawthorn species collected from Bolu province of Turkey was performed. Inter-priming binding sites (iPBS) markers based on retrotransposons were used for the first time in the molecular proper...
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MDPI AG
2020-11-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/10/11/1731 |
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| author | Aysen Gurlen Muttalip Gundogdu Goksel Ozer Sezai Ercisli Boris Duralija |
| author_facet | Aysen Gurlen Muttalip Gundogdu Goksel Ozer Sezai Ercisli Boris Duralija |
| author_sort | Aysen Gurlen |
| collection | DOAJ |
| description | In this study, the molecular, biochemical and agro-morphological characterization of genotypes belonging to hawthorn species collected from Bolu province of Turkey was performed. Inter-priming binding sites (iPBS) markers based on retrotransposons were used for the first time in the molecular properties of hawthorn genotypes in the world. The marker system provided very useful information for revealing the genetic variation of the genotypes. Six iPBS markers amplified 68 fragments, of which 65 were polymorphic (95.59%) with an average of 10.83 polymorphic bands per primer. The polymorphism and resolving power per primers ranged from 0.12 to 0.42 and from 0.78 to 8.11 with the average being 0.32 and 5.95, respectively. Pomological properties of <i>Crataegus tanacetifolia</i>, such as fruit pomology and core weight were determined to higher than those of <i>Crataegus monogyna</i>. Citric acid was determined as the most predominant organic acid, followed by malic and succinic acid in the genotypes of both species. The highest citric acid content (26.745 mg 100 g<sup>−1</sup>) was noted for 14BL09 genotype. The vit. C content was recorded ranging from 2.681 to 9.621 mg 100 g<sup>−1</sup>. Catechin, chlorogenic, caffeic and rutin contents were varied between 4.140–51.393 mg, 2.254–42.361 mg, 0.624–4.407 mg, and 1.241–10.029 mg per 100 g of fruits, respectively. As a result, it has been determined that twenty-five genotypes belonging to different hawthorn species are important genetic resources to be evaluated in horticultural breeding studies in terms of their physical and biochemical contents. |
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| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-10T15:02:13Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-80495f21238542b89a9576961e8573592023-11-20T20:04:14ZengMDPI AGAgronomy2073-43952020-11-011011173110.3390/agronomy10111731Primary, Secondary Metabolites and Molecular Characterization of Hawthorn (<i>Crataegus</i> spp.) GenotypesAysen Gurlen0Muttalip Gundogdu1Goksel Ozer2Sezai Ercisli3Boris Duralija4Bolu Directorate of Provincial Agriculture and Forestry, Bolu 14600, TurkeyDepartment of Horticulture, Agriculture and Natural Sciences Faculty, Bolu Abant Izzet Baysal University, Bolu 14030, TurkeyDepartment of Plant Protection, Agriculture and Natural Sciences Faculty, Bolu Abant Izzet Baysal University, Bolu 14030, TurkeyDepartment of Horticulture, Agricultural Faculty, Ataturk University, Erzurum 25240, TurkeyDepartment of Pomology, Faculty of Agriculture, University of Zagreb, 10000 Zagreb, CroatiaIn this study, the molecular, biochemical and agro-morphological characterization of genotypes belonging to hawthorn species collected from Bolu province of Turkey was performed. Inter-priming binding sites (iPBS) markers based on retrotransposons were used for the first time in the molecular properties of hawthorn genotypes in the world. The marker system provided very useful information for revealing the genetic variation of the genotypes. Six iPBS markers amplified 68 fragments, of which 65 were polymorphic (95.59%) with an average of 10.83 polymorphic bands per primer. The polymorphism and resolving power per primers ranged from 0.12 to 0.42 and from 0.78 to 8.11 with the average being 0.32 and 5.95, respectively. Pomological properties of <i>Crataegus tanacetifolia</i>, such as fruit pomology and core weight were determined to higher than those of <i>Crataegus monogyna</i>. Citric acid was determined as the most predominant organic acid, followed by malic and succinic acid in the genotypes of both species. The highest citric acid content (26.745 mg 100 g<sup>−1</sup>) was noted for 14BL09 genotype. The vit. C content was recorded ranging from 2.681 to 9.621 mg 100 g<sup>−1</sup>. Catechin, chlorogenic, caffeic and rutin contents were varied between 4.140–51.393 mg, 2.254–42.361 mg, 0.624–4.407 mg, and 1.241–10.029 mg per 100 g of fruits, respectively. As a result, it has been determined that twenty-five genotypes belonging to different hawthorn species are important genetic resources to be evaluated in horticultural breeding studies in terms of their physical and biochemical contents.https://www.mdpi.com/2073-4395/10/11/1731hawthornretrotransposonsorganic acidsphenolic compoundspomology |
| spellingShingle | Aysen Gurlen Muttalip Gundogdu Goksel Ozer Sezai Ercisli Boris Duralija Primary, Secondary Metabolites and Molecular Characterization of Hawthorn (<i>Crataegus</i> spp.) Genotypes Agronomy hawthorn retrotransposons organic acids phenolic compounds pomology |
| title | Primary, Secondary Metabolites and Molecular Characterization of Hawthorn (<i>Crataegus</i> spp.) Genotypes |
| title_full | Primary, Secondary Metabolites and Molecular Characterization of Hawthorn (<i>Crataegus</i> spp.) Genotypes |
| title_fullStr | Primary, Secondary Metabolites and Molecular Characterization of Hawthorn (<i>Crataegus</i> spp.) Genotypes |
| title_full_unstemmed | Primary, Secondary Metabolites and Molecular Characterization of Hawthorn (<i>Crataegus</i> spp.) Genotypes |
| title_short | Primary, Secondary Metabolites and Molecular Characterization of Hawthorn (<i>Crataegus</i> spp.) Genotypes |
| title_sort | primary secondary metabolites and molecular characterization of hawthorn i crataegus i spp genotypes |
| topic | hawthorn retrotransposons organic acids phenolic compounds pomology |
| url | https://www.mdpi.com/2073-4395/10/11/1731 |
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