Analysis of epicardial genes in embryonic mouse hearts with flow cytometry
Summary: Genetic markers used to define discrete cell populations are seldom expressed exclusively in the population of interest and are, thus, unsuitable when evaluated individually, especially in the absence of spatial and morphological information. Here, we present fluorescence in situ hybridizat...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-03-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166721000666 |
| _version_ | 1818336619047944192 |
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| author | Andia Nicole Redpath Irina-Elena Lupu Nicola Smart |
| author_facet | Andia Nicole Redpath Irina-Elena Lupu Nicola Smart |
| author_sort | Andia Nicole Redpath |
| collection | DOAJ |
| description | Summary: Genetic markers used to define discrete cell populations are seldom expressed exclusively in the population of interest and are, thus, unsuitable when evaluated individually, especially in the absence of spatial and morphological information. Here, we present fluorescence in situ hybridization for flow cytometry to allow simultaneous analysis of multiple marker genes at the single whole-cell level, exemplified by application to the embryonic epicardium. The protocol facilitates multiplexed quantification of gene and protein expression and temporal changes across specific cell populations.For complete details on the use and execution of this protocol, please refer to Lupu et al. (2020). |
| first_indexed | 2024-12-13T14:42:12Z |
| format | Article |
| id | doaj.art-804bebd3af184631a475efc7031c679d |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-12-13T14:42:12Z |
| publishDate | 2021-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-804bebd3af184631a475efc7031c679d2022-12-21T23:41:35ZengElsevierSTAR Protocols2666-16672021-03-0121100359Analysis of epicardial genes in embryonic mouse hearts with flow cytometryAndia Nicole Redpath0Irina-Elena Lupu1Nicola Smart2British Heart Foundation Centre of Regenerative Medicine, Burdon Sanderson Cardiac Science Centre, Department of Physiology, Anatomy & Genetics, University of Oxford, South Parks Road, Oxford OX1 3PT, UK; Corresponding authorBritish Heart Foundation Centre of Regenerative Medicine, Burdon Sanderson Cardiac Science Centre, Department of Physiology, Anatomy & Genetics, University of Oxford, South Parks Road, Oxford OX1 3PT, UKBritish Heart Foundation Centre of Regenerative Medicine, Burdon Sanderson Cardiac Science Centre, Department of Physiology, Anatomy & Genetics, University of Oxford, South Parks Road, Oxford OX1 3PT, UK; Corresponding authorSummary: Genetic markers used to define discrete cell populations are seldom expressed exclusively in the population of interest and are, thus, unsuitable when evaluated individually, especially in the absence of spatial and morphological information. Here, we present fluorescence in situ hybridization for flow cytometry to allow simultaneous analysis of multiple marker genes at the single whole-cell level, exemplified by application to the embryonic epicardium. The protocol facilitates multiplexed quantification of gene and protein expression and temporal changes across specific cell populations.For complete details on the use and execution of this protocol, please refer to Lupu et al. (2020).http://www.sciencedirect.com/science/article/pii/S2666166721000666Cell isolationFlow cytometry/mass cytometryGene expressionAntibodyIn situ hybridization |
| spellingShingle | Andia Nicole Redpath Irina-Elena Lupu Nicola Smart Analysis of epicardial genes in embryonic mouse hearts with flow cytometry STAR Protocols Cell isolation Flow cytometry/mass cytometry Gene expression Antibody In situ hybridization |
| title | Analysis of epicardial genes in embryonic mouse hearts with flow cytometry |
| title_full | Analysis of epicardial genes in embryonic mouse hearts with flow cytometry |
| title_fullStr | Analysis of epicardial genes in embryonic mouse hearts with flow cytometry |
| title_full_unstemmed | Analysis of epicardial genes in embryonic mouse hearts with flow cytometry |
| title_short | Analysis of epicardial genes in embryonic mouse hearts with flow cytometry |
| title_sort | analysis of epicardial genes in embryonic mouse hearts with flow cytometry |
| topic | Cell isolation Flow cytometry/mass cytometry Gene expression Antibody In situ hybridization |
| url | http://www.sciencedirect.com/science/article/pii/S2666166721000666 |
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