Efficient Separation of Phytochemicals from <i>Muehlenbeckia volcanica</i> (Benth.) Endl. by Polarity-Stepwise Elution Counter-Current Chromatography and Their Antioxidant, Antiglycation, and Aldose Reductase Inhibition Potentials
<i>Muehlenbeckia volcanica</i> (Benth.) Endl. (<i>M. volcanica</i>), native to South America, is a traditional Peruvian medicinal plant that has multi-therapeutic properties; however, no phytochemicals have been identified from it yet. In this study, a five-step polarity-step...
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| Format: | Article |
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MDPI AG
2021-01-01
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| Series: | Molecules |
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| author | Guang-Lei Zuo Hyun Yong Kim Yanymee N. Guillen Quispe Zhi-Qiang Wang Seung Hwan Hwang Kyong-Oh Shin Soon Sung Lim |
| author_facet | Guang-Lei Zuo Hyun Yong Kim Yanymee N. Guillen Quispe Zhi-Qiang Wang Seung Hwan Hwang Kyong-Oh Shin Soon Sung Lim |
| author_sort | Guang-Lei Zuo |
| collection | DOAJ |
| description | <i>Muehlenbeckia volcanica</i> (Benth.) Endl. (<i>M. volcanica</i>), native to South America, is a traditional Peruvian medicinal plant that has multi-therapeutic properties; however, no phytochemicals have been identified from it yet. In this study, a five-step polarity-stepwise elution counter-current chromatography (CCC) was developed using methanol/water (1:5, <i>v</i>/<i>v</i>) as the stationary phase and different ratios of <i>n</i>-hexane, ethyl acetate, and <i>n</i>-butanol as mobile phases to separate the compounds from the 70% methanol extract of <i>M. volcanica,</i> by which six compounds with a wide range of polarities were separated in a single run of CCC and were identified as gallic acid, protocatechuic acid, 4,4′-dihydroxy-3,3′-imino-di-benzoic acid, rutin, quercitrin, and quercetin. Then, two compounds from the fractions of stepwise elution CCC were separated using conventional high-speed CCC, pH-zone-refining CCC, and preparative high-performance liquid chromatography, and identified as shikimic acid and miquelianin. These compounds are reported from <i>M. volcanica</i> for the first time. Notably, except for shikimic acid, all other compounds showed anti-diabetic potentials via antioxidant, antiglycation, and aldose reductase inhibition. The results suggest that the polarity-stepwise elution CCC can be used to efficiently separate or fractionate compounds with a wide range of polarities from natural products. Moreover, <i>M. volcanica</i> and its bioactive compounds are potent anti-diabetic agents. |
| first_indexed | 2024-03-10T13:30:04Z |
| format | Article |
| id | doaj.art-804c683dce7b42d18e95bf845b5ff491 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T13:30:04Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-804c683dce7b42d18e95bf845b5ff4912023-11-21T08:10:41ZengMDPI AGMolecules1420-30492021-01-0126122410.3390/molecules26010224Efficient Separation of Phytochemicals from <i>Muehlenbeckia volcanica</i> (Benth.) Endl. by Polarity-Stepwise Elution Counter-Current Chromatography and Their Antioxidant, Antiglycation, and Aldose Reductase Inhibition PotentialsGuang-Lei Zuo0Hyun Yong Kim1Yanymee N. Guillen Quispe2Zhi-Qiang Wang3Seung Hwan Hwang4Kyong-Oh Shin5Soon Sung Lim6Department of Food Science and Nutrition, Hallym University, 1 Hallymdeahak-gil, Chuncheon 24252, KoreaDepartment of Food Science and Nutrition, Hallym University, 1 Hallymdeahak-gil, Chuncheon 24252, KoreaDepartment of Food Science and Nutrition, Hallym University, 1 Hallymdeahak-gil, Chuncheon 24252, KoreaDepartment of Food Science and Nutrition, Hallym University, 1 Hallymdeahak-gil, Chuncheon 24252, KoreaDepartment of Food Science and Nutrition, Hallym University, 1 Hallymdeahak-gil, Chuncheon 24252, KoreaDepartment of Food Science and Nutrition, Hallym University, 1 Hallymdeahak-gil, Chuncheon 24252, KoreaDepartment of Food Science and Nutrition, Hallym University, 1 Hallymdeahak-gil, Chuncheon 24252, Korea<i>Muehlenbeckia volcanica</i> (Benth.) Endl. (<i>M. volcanica</i>), native to South America, is a traditional Peruvian medicinal plant that has multi-therapeutic properties; however, no phytochemicals have been identified from it yet. In this study, a five-step polarity-stepwise elution counter-current chromatography (CCC) was developed using methanol/water (1:5, <i>v</i>/<i>v</i>) as the stationary phase and different ratios of <i>n</i>-hexane, ethyl acetate, and <i>n</i>-butanol as mobile phases to separate the compounds from the 70% methanol extract of <i>M. volcanica,</i> by which six compounds with a wide range of polarities were separated in a single run of CCC and were identified as gallic acid, protocatechuic acid, 4,4′-dihydroxy-3,3′-imino-di-benzoic acid, rutin, quercitrin, and quercetin. Then, two compounds from the fractions of stepwise elution CCC were separated using conventional high-speed CCC, pH-zone-refining CCC, and preparative high-performance liquid chromatography, and identified as shikimic acid and miquelianin. These compounds are reported from <i>M. volcanica</i> for the first time. Notably, except for shikimic acid, all other compounds showed anti-diabetic potentials via antioxidant, antiglycation, and aldose reductase inhibition. The results suggest that the polarity-stepwise elution CCC can be used to efficiently separate or fractionate compounds with a wide range of polarities from natural products. Moreover, <i>M. volcanica</i> and its bioactive compounds are potent anti-diabetic agents.https://www.mdpi.com/1420-3049/26/1/224antioxidantantiglycationaldose reductase inhibitioncounter-current chromatography<i>Muehlenbeckia volcanica</i> (Benth.) Endl.stepwise elution |
| spellingShingle | Guang-Lei Zuo Hyun Yong Kim Yanymee N. Guillen Quispe Zhi-Qiang Wang Seung Hwan Hwang Kyong-Oh Shin Soon Sung Lim Efficient Separation of Phytochemicals from <i>Muehlenbeckia volcanica</i> (Benth.) Endl. by Polarity-Stepwise Elution Counter-Current Chromatography and Their Antioxidant, Antiglycation, and Aldose Reductase Inhibition Potentials Molecules antioxidant antiglycation aldose reductase inhibition counter-current chromatography <i>Muehlenbeckia volcanica</i> (Benth.) Endl. stepwise elution |
| title | Efficient Separation of Phytochemicals from <i>Muehlenbeckia volcanica</i> (Benth.) Endl. by Polarity-Stepwise Elution Counter-Current Chromatography and Their Antioxidant, Antiglycation, and Aldose Reductase Inhibition Potentials |
| title_full | Efficient Separation of Phytochemicals from <i>Muehlenbeckia volcanica</i> (Benth.) Endl. by Polarity-Stepwise Elution Counter-Current Chromatography and Their Antioxidant, Antiglycation, and Aldose Reductase Inhibition Potentials |
| title_fullStr | Efficient Separation of Phytochemicals from <i>Muehlenbeckia volcanica</i> (Benth.) Endl. by Polarity-Stepwise Elution Counter-Current Chromatography and Their Antioxidant, Antiglycation, and Aldose Reductase Inhibition Potentials |
| title_full_unstemmed | Efficient Separation of Phytochemicals from <i>Muehlenbeckia volcanica</i> (Benth.) Endl. by Polarity-Stepwise Elution Counter-Current Chromatography and Their Antioxidant, Antiglycation, and Aldose Reductase Inhibition Potentials |
| title_short | Efficient Separation of Phytochemicals from <i>Muehlenbeckia volcanica</i> (Benth.) Endl. by Polarity-Stepwise Elution Counter-Current Chromatography and Their Antioxidant, Antiglycation, and Aldose Reductase Inhibition Potentials |
| title_sort | efficient separation of phytochemicals from i muehlenbeckia volcanica i benth endl by polarity stepwise elution counter current chromatography and their antioxidant antiglycation and aldose reductase inhibition potentials |
| topic | antioxidant antiglycation aldose reductase inhibition counter-current chromatography <i>Muehlenbeckia volcanica</i> (Benth.) Endl. stepwise elution |
| url | https://www.mdpi.com/1420-3049/26/1/224 |
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