Photosynthesis in rice is increased by CRISPR/Cas9-mediated transformation of two truncated light-harvesting antenna
Plants compete for light partly by over-producing chlorophyll in leaves. The resulting high light absorption is an effective strategy for out competing neighbors in mixed communities, but it prevents light transmission to lower leaves and limits photosynthesis in dense agricultural canopies. We used...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-01-01
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| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2023.1050483/full |
| _version_ | 1828059126900457472 |
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| author | Daniel Caddell Daniel Caddell Noah J. Langenfeld Madigan JH. Eckels Shuyang Zhen Rachel Klaras Laxmi Mishra Bruce Bugbee Devin Coleman-Derr Devin Coleman-Derr |
| author_facet | Daniel Caddell Daniel Caddell Noah J. Langenfeld Madigan JH. Eckels Shuyang Zhen Rachel Klaras Laxmi Mishra Bruce Bugbee Devin Coleman-Derr Devin Coleman-Derr |
| author_sort | Daniel Caddell |
| collection | DOAJ |
| description | Plants compete for light partly by over-producing chlorophyll in leaves. The resulting high light absorption is an effective strategy for out competing neighbors in mixed communities, but it prevents light transmission to lower leaves and limits photosynthesis in dense agricultural canopies. We used a CRISPR/Cas9-mediated approach to engineer rice plants with truncated light-harvesting antenna (TLA) via knockout mutations to individual antenna assembly component genes CpSRP43, CpSRP54a, and its paralog, CpSRP54b. We compared the photosynthetic contributions of these components in rice by studying the growth rates of whole plants, quantum yield of photosynthesis, chlorophyll density and distribution, and phenotypic abnormalities. Additionally, we investigated a Poales-specific duplication of CpSRP54. The Poales are an important family that includes staple crops such as rice, wheat, corn, millet, and sorghum. Mutations in any of these three genes involved in antenna assembly decreased chlorophyll content and light absorption and increased photosynthesis per photon absorbed (quantum yield). These results have significant implications for the improvement of high leaf-area-index crop monocultures. |
| first_indexed | 2024-04-10T21:38:11Z |
| format | Article |
| id | doaj.art-804d025c29234353978444c8ced3a056 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-10T21:38:11Z |
| publishDate | 2023-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-804d025c29234353978444c8ced3a0562023-01-19T08:38:21ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-01-011410.3389/fpls.2023.10504831050483Photosynthesis in rice is increased by CRISPR/Cas9-mediated transformation of two truncated light-harvesting antennaDaniel Caddell0Daniel Caddell1Noah J. Langenfeld2Madigan JH. Eckels3Shuyang Zhen4Rachel Klaras5Laxmi Mishra6Bruce Bugbee7Devin Coleman-Derr8Devin Coleman-Derr9Plant Gene Expression Center, United States Department of Agriculture - Agricultural Research Service (USDA ARS), Albany, CA, United StatesPlant and Microbial Biology Department, University of California at Berkeley, Berkeley, CA, United StatesDepartment of Plants, Soils, and Climate, Utah State University, Logan, UT, United StatesDepartment of Plants, Soils, and Climate, Utah State University, Logan, UT, United StatesDepartment of Horticultural Sciences, Texas A&M University, College Station, TX, United StatesPlant and Microbial Biology Department, University of California at Berkeley, Berkeley, CA, United StatesPlant and Microbial Biology Department, University of California at Berkeley, Berkeley, CA, United StatesDepartment of Plants, Soils, and Climate, Utah State University, Logan, UT, United StatesPlant Gene Expression Center, United States Department of Agriculture - Agricultural Research Service (USDA ARS), Albany, CA, United StatesPlant and Microbial Biology Department, University of California at Berkeley, Berkeley, CA, United StatesPlants compete for light partly by over-producing chlorophyll in leaves. The resulting high light absorption is an effective strategy for out competing neighbors in mixed communities, but it prevents light transmission to lower leaves and limits photosynthesis in dense agricultural canopies. We used a CRISPR/Cas9-mediated approach to engineer rice plants with truncated light-harvesting antenna (TLA) via knockout mutations to individual antenna assembly component genes CpSRP43, CpSRP54a, and its paralog, CpSRP54b. We compared the photosynthetic contributions of these components in rice by studying the growth rates of whole plants, quantum yield of photosynthesis, chlorophyll density and distribution, and phenotypic abnormalities. Additionally, we investigated a Poales-specific duplication of CpSRP54. The Poales are an important family that includes staple crops such as rice, wheat, corn, millet, and sorghum. Mutations in any of these three genes involved in antenna assembly decreased chlorophyll content and light absorption and increased photosynthesis per photon absorbed (quantum yield). These results have significant implications for the improvement of high leaf-area-index crop monocultures.https://www.frontiersin.org/articles/10.3389/fpls.2023.1050483/fulltruncated light antennaphotosynthesischlorophyllCRISPR/Cas9rice |
| spellingShingle | Daniel Caddell Daniel Caddell Noah J. Langenfeld Madigan JH. Eckels Shuyang Zhen Rachel Klaras Laxmi Mishra Bruce Bugbee Devin Coleman-Derr Devin Coleman-Derr Photosynthesis in rice is increased by CRISPR/Cas9-mediated transformation of two truncated light-harvesting antenna Frontiers in Plant Science truncated light antenna photosynthesis chlorophyll CRISPR/Cas9 rice |
| title | Photosynthesis in rice is increased by CRISPR/Cas9-mediated transformation of two truncated light-harvesting antenna |
| title_full | Photosynthesis in rice is increased by CRISPR/Cas9-mediated transformation of two truncated light-harvesting antenna |
| title_fullStr | Photosynthesis in rice is increased by CRISPR/Cas9-mediated transformation of two truncated light-harvesting antenna |
| title_full_unstemmed | Photosynthesis in rice is increased by CRISPR/Cas9-mediated transformation of two truncated light-harvesting antenna |
| title_short | Photosynthesis in rice is increased by CRISPR/Cas9-mediated transformation of two truncated light-harvesting antenna |
| title_sort | photosynthesis in rice is increased by crispr cas9 mediated transformation of two truncated light harvesting antenna |
| topic | truncated light antenna photosynthesis chlorophyll CRISPR/Cas9 rice |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2023.1050483/full |
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