Ubiquitin transfer by a RING E3 ligase occurs from a closed E2~ubiquitin conformation
The mechanism of ubiquitin transfer from the ubiquitin-conjugated E2 enzyme (E2~Ub) to the substrate is still under debate. Here, the authors use FRET assays to show that RING E3 ligases transfer ubiquitin to the substrate from a closed E2~Ub conformation.
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2020-06-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-020-16666-y |
| _version_ | 1818683300356554752 |
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| author | Emma Branigan J. Carlos Penedo Ronald T. Hay |
| author_facet | Emma Branigan J. Carlos Penedo Ronald T. Hay |
| author_sort | Emma Branigan |
| collection | DOAJ |
| description | The mechanism of ubiquitin transfer from the ubiquitin-conjugated E2 enzyme (E2~Ub) to the substrate is still under debate. Here, the authors use FRET assays to show that RING E3 ligases transfer ubiquitin to the substrate from a closed E2~Ub conformation. |
| first_indexed | 2024-12-17T10:32:33Z |
| format | Article |
| id | doaj.art-804ead47bc3a4058bfc76e0bf7df8968 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-12-17T10:32:33Z |
| publishDate | 2020-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-804ead47bc3a4058bfc76e0bf7df89682022-12-21T21:52:29ZengNature PortfolioNature Communications2041-17232020-06-0111111110.1038/s41467-020-16666-yUbiquitin transfer by a RING E3 ligase occurs from a closed E2~ubiquitin conformationEmma Branigan0J. Carlos Penedo1Ronald T. Hay2Centre for Gene Regulation and Expression, School of Life Sciences, University of DundeeCentre of Biophotonics, School of Physics and Astronomy, University of St. AndrewsCentre for Gene Regulation and Expression, School of Life Sciences, University of DundeeThe mechanism of ubiquitin transfer from the ubiquitin-conjugated E2 enzyme (E2~Ub) to the substrate is still under debate. Here, the authors use FRET assays to show that RING E3 ligases transfer ubiquitin to the substrate from a closed E2~Ub conformation.https://doi.org/10.1038/s41467-020-16666-y |
| spellingShingle | Emma Branigan J. Carlos Penedo Ronald T. Hay Ubiquitin transfer by a RING E3 ligase occurs from a closed E2~ubiquitin conformation Nature Communications |
| title | Ubiquitin transfer by a RING E3 ligase occurs from a closed E2~ubiquitin conformation |
| title_full | Ubiquitin transfer by a RING E3 ligase occurs from a closed E2~ubiquitin conformation |
| title_fullStr | Ubiquitin transfer by a RING E3 ligase occurs from a closed E2~ubiquitin conformation |
| title_full_unstemmed | Ubiquitin transfer by a RING E3 ligase occurs from a closed E2~ubiquitin conformation |
| title_short | Ubiquitin transfer by a RING E3 ligase occurs from a closed E2~ubiquitin conformation |
| title_sort | ubiquitin transfer by a ring e3 ligase occurs from a closed e2 ubiquitin conformation |
| url | https://doi.org/10.1038/s41467-020-16666-y |
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