Transcriptome profiles of high-lysine adaptation reveal insights into osmotic stress response in Corynebacterium glutamicum
Corynebacterium glutamicum has been widely and effectively used for fermentative production of l-lysine on an industrial scale. However, high-level accumulation of end products inevitably leads to osmotic stress and hinders further increase of l-lysine production. At present, the underlying mechanis...
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| Format: | Article |
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Frontiers Media S.A.
2022-08-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2022.933325/full |
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| author | Jian Wang Jian Yang Guoxin Shi Weidong Li Yun Ju Liang Wei Jun Liu Jun Liu Ning Xu Ning Xu |
| author_facet | Jian Wang Jian Yang Guoxin Shi Weidong Li Yun Ju Liang Wei Jun Liu Jun Liu Ning Xu Ning Xu |
| author_sort | Jian Wang |
| collection | DOAJ |
| description | Corynebacterium glutamicum has been widely and effectively used for fermentative production of l-lysine on an industrial scale. However, high-level accumulation of end products inevitably leads to osmotic stress and hinders further increase of l-lysine production. At present, the underlying mechanism by which C. glutamicum cells adapt to high-lysine-induced osmotic stress is still unclear. In this study, we conducted a comparative transcriptomic analysis by RNA-seq to determine gene expression profiles under different high-lysine stress conditions. The results indicated that the increased expression of some metabolic pathways such as sulfur metabolism and specific amino acid biosynthesis might offer favorable benefits for high-lysine adaptation. Functional assays of 18 representative differentially expressed genes showed that the enhanced expression of multiple candidate genes, especially grpE chaperon, conferred high-lysine stress tolerance in C. glutamicum. Moreover, DNA repair component MutT and energy-transducing NADH dehydrogenase Ndh were also found to be important for protecting cells against high-lysine-induced osmotic stress. Taken together, these aforementioned findings provide broader views of transcriptome profiles and promising candidate targets of C. glutamicum for the adaptation of high-lysine stress during fermentation. |
| first_indexed | 2024-04-13T19:02:31Z |
| format | Article |
| id | doaj.art-80527a3b61e3427f8a404f9eebd8fe0e |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-04-13T19:02:31Z |
| publishDate | 2022-08-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-80527a3b61e3427f8a404f9eebd8fe0e2022-12-22T02:34:03ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852022-08-011010.3389/fbioe.2022.933325933325Transcriptome profiles of high-lysine adaptation reveal insights into osmotic stress response in Corynebacterium glutamicumJian Wang0Jian Yang1Guoxin Shi2Weidong Li3Yun Ju4Liang Wei5Jun Liu6Jun Liu7Ning Xu8Ning Xu9College of Biological and Agricultural Engineering, Jilin University, Changchun, ChinaCollege of Biological and Agricultural Engineering, Jilin University, Changchun, ChinaCollege of Biological and Agricultural Engineering, Jilin University, Changchun, ChinaCollege of Biological and Agricultural Engineering, Jilin University, Changchun, ChinaSchool of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin, ChinaTianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, ChinaTianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, ChinaKey Laboratory of Systems Microbial Biotechnology, Chinese Academy of Sciences, Tianjin, ChinaTianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, ChinaKey Laboratory of Systems Microbial Biotechnology, Chinese Academy of Sciences, Tianjin, ChinaCorynebacterium glutamicum has been widely and effectively used for fermentative production of l-lysine on an industrial scale. However, high-level accumulation of end products inevitably leads to osmotic stress and hinders further increase of l-lysine production. At present, the underlying mechanism by which C. glutamicum cells adapt to high-lysine-induced osmotic stress is still unclear. In this study, we conducted a comparative transcriptomic analysis by RNA-seq to determine gene expression profiles under different high-lysine stress conditions. The results indicated that the increased expression of some metabolic pathways such as sulfur metabolism and specific amino acid biosynthesis might offer favorable benefits for high-lysine adaptation. Functional assays of 18 representative differentially expressed genes showed that the enhanced expression of multiple candidate genes, especially grpE chaperon, conferred high-lysine stress tolerance in C. glutamicum. Moreover, DNA repair component MutT and energy-transducing NADH dehydrogenase Ndh were also found to be important for protecting cells against high-lysine-induced osmotic stress. Taken together, these aforementioned findings provide broader views of transcriptome profiles and promising candidate targets of C. glutamicum for the adaptation of high-lysine stress during fermentation.https://www.frontiersin.org/articles/10.3389/fbioe.2022.933325/fulltranscriptome profileshigh-lysine stressmolecular chaperonDNA repairosmoadaptationC. glutamicum |
| spellingShingle | Jian Wang Jian Yang Guoxin Shi Weidong Li Yun Ju Liang Wei Jun Liu Jun Liu Ning Xu Ning Xu Transcriptome profiles of high-lysine adaptation reveal insights into osmotic stress response in Corynebacterium glutamicum Frontiers in Bioengineering and Biotechnology transcriptome profiles high-lysine stress molecular chaperon DNA repair osmoadaptation C. glutamicum |
| title | Transcriptome profiles of high-lysine adaptation reveal insights into osmotic stress response in Corynebacterium glutamicum |
| title_full | Transcriptome profiles of high-lysine adaptation reveal insights into osmotic stress response in Corynebacterium glutamicum |
| title_fullStr | Transcriptome profiles of high-lysine adaptation reveal insights into osmotic stress response in Corynebacterium glutamicum |
| title_full_unstemmed | Transcriptome profiles of high-lysine adaptation reveal insights into osmotic stress response in Corynebacterium glutamicum |
| title_short | Transcriptome profiles of high-lysine adaptation reveal insights into osmotic stress response in Corynebacterium glutamicum |
| title_sort | transcriptome profiles of high lysine adaptation reveal insights into osmotic stress response in corynebacterium glutamicum |
| topic | transcriptome profiles high-lysine stress molecular chaperon DNA repair osmoadaptation C. glutamicum |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2022.933325/full |
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