Chicken Combs and Wattles as Sources of Bioactive Peptides: Optimization of Hydrolysis, Identification by LC-ESI-MS<sup>2</sup> and Bioactivity Assessment
The production of bioactive peptides from organic by-waste materials is in line with current trends devoted to guaranteeing environmental protection and a circular economy. The objectives of this study were i) to optimize the conditions for obtaining bioactive hydrolysates from chicken combs and wat...
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MDPI AG
2020-04-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/25/7/1698 |
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| author | Taliana Bezerra Mario Estévez José Thalles Lacerda Meriellen Dias Maria Juliano Maria Anita Mendes Marcelo Morgano Maria Teresa Pacheco Marta Madruga |
| author_facet | Taliana Bezerra Mario Estévez José Thalles Lacerda Meriellen Dias Maria Juliano Maria Anita Mendes Marcelo Morgano Maria Teresa Pacheco Marta Madruga |
| author_sort | Taliana Bezerra |
| collection | DOAJ |
| description | The production of bioactive peptides from organic by-waste materials is in line with current trends devoted to guaranteeing environmental protection and a circular economy. The objectives of this study were i) to optimize the conditions for obtaining bioactive hydrolysates from chicken combs and wattles using Alcalase, ii) to identify the resulting peptides using LC-ESI-MS<sup>2</sup> and iii) to evaluate their chelating and antioxidant activities. The hydrolysate obtained using a ratio of enzyme to substrate of 5% (<i>w</i>/<i>w</i>) and 240 min of hydrolysis showed excellent Fe<sup>2+</sup> chelating and antioxidant capacities, reducing Fe<sup>3+</sup> and inhibiting 2, 2′-Azino-bis(3-ethylbenz-thiazoline-6-sulfonic acid) (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals. The mapping of ion distribution showed that a high degree of hydrolysis led to the production of peptides with <i>m</i>/<i>z</i> ≤ 400, suggesting low mass peptides or peptides with multiple charge precursor ions. The peptides derived from the proteins of cartilage like Collagen alpha-2(I), Collagen alpha-1(I), Collagen alpha-1(III) and elastin contributed to generation of bioactive compounds. Hydrolysates from chicken waste materials could be regarded as candidates to be used as ingredients to design processed foods with functional properties. |
| first_indexed | 2024-03-10T20:36:53Z |
| format | Article |
| id | doaj.art-805868bb2abc4ef899d6af5578d45f9e |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T20:36:53Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-805868bb2abc4ef899d6af5578d45f9e2023-11-19T20:57:19ZengMDPI AGMolecules1420-30492020-04-01257169810.3390/molecules25071698Chicken Combs and Wattles as Sources of Bioactive Peptides: Optimization of Hydrolysis, Identification by LC-ESI-MS<sup>2</sup> and Bioactivity AssessmentTaliana Bezerra0Mario Estévez1José Thalles Lacerda2Meriellen Dias3Maria Juliano4Maria Anita Mendes5Marcelo Morgano6Maria Teresa Pacheco7Marta Madruga8Department of Food Engineering, Technology Centre, Federal University of Paraiba, Joao Pessoa 58051-900, BrazilAnimal Production and Food Science Department, IPROCAR Research Institute, Avda Ciencias s/n, University of Extremadura, 10003 Cáceres, SpainDepartment of Biophysics Federal University of São Paulo, Rua Tres de Maio, 2 andar, São Paulo 100, BrazilDempster MS Lab-Chemical Engineering, Department of Polytechnic, School of University of Saõ Paulo, Saõ Paulo Rua do Lago 250, Bloco B 3, Saõ Paulo 05508-080, BrazilDepartment of Biophysics Federal University of São Paulo, Rua Tres de Maio, 2 andar, São Paulo 100, BrazilDempster MS Lab-Chemical Engineering, Department of Polytechnic, School of University of Saõ Paulo, Saõ Paulo Rua do Lago 250, Bloco B 3, Saõ Paulo 05508-080, BrazilInstitute of Food Technology (ITAL), Brasil Ave 2880, P.O. Box 139, Campinas 13070-178, BrazilInstitute of Food Technology (ITAL), Brasil Ave 2880, P.O. Box 139, Campinas 13070-178, BrazilDepartment of Food Engineering, Technology Centre, Federal University of Paraiba, Joao Pessoa 58051-900, BrazilThe production of bioactive peptides from organic by-waste materials is in line with current trends devoted to guaranteeing environmental protection and a circular economy. The objectives of this study were i) to optimize the conditions for obtaining bioactive hydrolysates from chicken combs and wattles using Alcalase, ii) to identify the resulting peptides using LC-ESI-MS<sup>2</sup> and iii) to evaluate their chelating and antioxidant activities. The hydrolysate obtained using a ratio of enzyme to substrate of 5% (<i>w</i>/<i>w</i>) and 240 min of hydrolysis showed excellent Fe<sup>2+</sup> chelating and antioxidant capacities, reducing Fe<sup>3+</sup> and inhibiting 2, 2′-Azino-bis(3-ethylbenz-thiazoline-6-sulfonic acid) (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals. The mapping of ion distribution showed that a high degree of hydrolysis led to the production of peptides with <i>m</i>/<i>z</i> ≤ 400, suggesting low mass peptides or peptides with multiple charge precursor ions. The peptides derived from the proteins of cartilage like Collagen alpha-2(I), Collagen alpha-1(I), Collagen alpha-1(III) and elastin contributed to generation of bioactive compounds. Hydrolysates from chicken waste materials could be regarded as candidates to be used as ingredients to design processed foods with functional properties.https://www.mdpi.com/1420-3049/25/7/1698protein hydrolysatefree radicalschicken by-productsbioactive compoundsLC-ESI-MS<sup>2</sup> |
| spellingShingle | Taliana Bezerra Mario Estévez José Thalles Lacerda Meriellen Dias Maria Juliano Maria Anita Mendes Marcelo Morgano Maria Teresa Pacheco Marta Madruga Chicken Combs and Wattles as Sources of Bioactive Peptides: Optimization of Hydrolysis, Identification by LC-ESI-MS<sup>2</sup> and Bioactivity Assessment Molecules protein hydrolysate free radicals chicken by-products bioactive compounds LC-ESI-MS<sup>2</sup> |
| title | Chicken Combs and Wattles as Sources of Bioactive Peptides: Optimization of Hydrolysis, Identification by LC-ESI-MS<sup>2</sup> and Bioactivity Assessment |
| title_full | Chicken Combs and Wattles as Sources of Bioactive Peptides: Optimization of Hydrolysis, Identification by LC-ESI-MS<sup>2</sup> and Bioactivity Assessment |
| title_fullStr | Chicken Combs and Wattles as Sources of Bioactive Peptides: Optimization of Hydrolysis, Identification by LC-ESI-MS<sup>2</sup> and Bioactivity Assessment |
| title_full_unstemmed | Chicken Combs and Wattles as Sources of Bioactive Peptides: Optimization of Hydrolysis, Identification by LC-ESI-MS<sup>2</sup> and Bioactivity Assessment |
| title_short | Chicken Combs and Wattles as Sources of Bioactive Peptides: Optimization of Hydrolysis, Identification by LC-ESI-MS<sup>2</sup> and Bioactivity Assessment |
| title_sort | chicken combs and wattles as sources of bioactive peptides optimization of hydrolysis identification by lc esi ms sup 2 sup and bioactivity assessment |
| topic | protein hydrolysate free radicals chicken by-products bioactive compounds LC-ESI-MS<sup>2</sup> |
| url | https://www.mdpi.com/1420-3049/25/7/1698 |
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