Natural IgG against S-Protein and RBD of SARS-CoV-2 Do Not Bind and Hydrolyze DNA and Are Not Autoimmune
Since the onset of the COVID-19 pandemic, numerous publications have appeared describing autoimmune pathologies developing after a coronavirus infection, with several papers reporting autoantibody production during the acute period of the disease. Several viral diseases are known to trigger autoimmu...
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MDPI AG
2022-11-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/22/13681 |
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| author | Anna M. Timofeeva Sergey E. Sedykh Evgeny A. Ermakov Andrey L. Matveev Eva I. Odegova Tatiana A. Sedykh Dmitry N. Shcherbakov Iuliia A. Merkuleva Ekaterina A. Volosnikova Valentina S. Nesmeyanova Nina V. Tikunova Georgy A. Nevinsky |
| author_facet | Anna M. Timofeeva Sergey E. Sedykh Evgeny A. Ermakov Andrey L. Matveev Eva I. Odegova Tatiana A. Sedykh Dmitry N. Shcherbakov Iuliia A. Merkuleva Ekaterina A. Volosnikova Valentina S. Nesmeyanova Nina V. Tikunova Georgy A. Nevinsky |
| author_sort | Anna M. Timofeeva |
| collection | DOAJ |
| description | Since the onset of the COVID-19 pandemic, numerous publications have appeared describing autoimmune pathologies developing after a coronavirus infection, with several papers reporting autoantibody production during the acute period of the disease. Several viral diseases are known to trigger autoimmune processes, and the appearance of catalytic antibodies with DNase activity is one of the earliest markers of several autoimmune pathologies. Therefore, we analyzed whether IgG antibodies from blood plasma of SARS-CoV-2 patients after recovery could bind and hydrolyze DNA. We analyzed how vaccination of patients with adenovirus Sputnik V vaccine influences the production of abzymes with DNase activity. Four groups were selected for the analysis, each containing 25 patients according to their relative titers of antibodies to S-protein: with high and median titers, vaccinated with Sputnik V with high titers, and a control group of donors with negative titers. The relative titers of antibodies against DNA and the relative DNase activity of IgGs depended very much on the individual patient and the donor, and no significant correlation was found between the relative values of antibodies titers and their DNase activity. Our results indicate that COVID-19 disease and vaccination with adenoviral Sputnik V vaccine do not result in the development or enhancement of strong autoimmune reactions as in the typical autoimmune diseases associated with the production of anti-DNA and DNA hydrolyzing antibodies. |
| first_indexed | 2024-03-09T18:18:43Z |
| format | Article |
| id | doaj.art-805c8c64e25d4ae3aa5268796448f3f0 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T18:18:43Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-805c8c64e25d4ae3aa5268796448f3f02023-11-24T08:31:40ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-11-0123221368110.3390/ijms232213681Natural IgG against S-Protein and RBD of SARS-CoV-2 Do Not Bind and Hydrolyze DNA and Are Not AutoimmuneAnna M. Timofeeva0Sergey E. Sedykh1Evgeny A. Ermakov2Andrey L. Matveev3Eva I. Odegova4Tatiana A. Sedykh5Dmitry N. Shcherbakov6Iuliia A. Merkuleva7Ekaterina A. Volosnikova8Valentina S. Nesmeyanova9Nina V. Tikunova10Georgy A. Nevinsky11SB RAS Institute of Chemical Biology and Fundamental Medicine, 630090 Novosibirsk, RussiaSB RAS Institute of Chemical Biology and Fundamental Medicine, 630090 Novosibirsk, RussiaSB RAS Institute of Chemical Biology and Fundamental Medicine, 630090 Novosibirsk, RussiaSB RAS Institute of Chemical Biology and Fundamental Medicine, 630090 Novosibirsk, RussiaSB RAS Institute of Chemical Biology and Fundamental Medicine, 630090 Novosibirsk, RussiaSB RAS Institute of Chemical Biology and Fundamental Medicine, 630090 Novosibirsk, RussiaState Research Center of Virology and Biotechnology Vector, 630559 Koltsovo, RussiaState Research Center of Virology and Biotechnology Vector, 630559 Koltsovo, RussiaState Research Center of Virology and Biotechnology Vector, 630559 Koltsovo, RussiaState Research Center of Virology and Biotechnology Vector, 630559 Koltsovo, RussiaSB RAS Institute of Chemical Biology and Fundamental Medicine, 630090 Novosibirsk, RussiaSB RAS Institute of Chemical Biology and Fundamental Medicine, 630090 Novosibirsk, RussiaSince the onset of the COVID-19 pandemic, numerous publications have appeared describing autoimmune pathologies developing after a coronavirus infection, with several papers reporting autoantibody production during the acute period of the disease. Several viral diseases are known to trigger autoimmune processes, and the appearance of catalytic antibodies with DNase activity is one of the earliest markers of several autoimmune pathologies. Therefore, we analyzed whether IgG antibodies from blood plasma of SARS-CoV-2 patients after recovery could bind and hydrolyze DNA. We analyzed how vaccination of patients with adenovirus Sputnik V vaccine influences the production of abzymes with DNase activity. Four groups were selected for the analysis, each containing 25 patients according to their relative titers of antibodies to S-protein: with high and median titers, vaccinated with Sputnik V with high titers, and a control group of donors with negative titers. The relative titers of antibodies against DNA and the relative DNase activity of IgGs depended very much on the individual patient and the donor, and no significant correlation was found between the relative values of antibodies titers and their DNase activity. Our results indicate that COVID-19 disease and vaccination with adenoviral Sputnik V vaccine do not result in the development or enhancement of strong autoimmune reactions as in the typical autoimmune diseases associated with the production of anti-DNA and DNA hydrolyzing antibodies.https://www.mdpi.com/1422-0067/23/22/13681SARS-CoV-2COVID-19anti-dsDNA-IgGsautoimmunityspike proteinRBD |
| spellingShingle | Anna M. Timofeeva Sergey E. Sedykh Evgeny A. Ermakov Andrey L. Matveev Eva I. Odegova Tatiana A. Sedykh Dmitry N. Shcherbakov Iuliia A. Merkuleva Ekaterina A. Volosnikova Valentina S. Nesmeyanova Nina V. Tikunova Georgy A. Nevinsky Natural IgG against S-Protein and RBD of SARS-CoV-2 Do Not Bind and Hydrolyze DNA and Are Not Autoimmune International Journal of Molecular Sciences SARS-CoV-2 COVID-19 anti-dsDNA-IgGs autoimmunity spike protein RBD |
| title | Natural IgG against S-Protein and RBD of SARS-CoV-2 Do Not Bind and Hydrolyze DNA and Are Not Autoimmune |
| title_full | Natural IgG against S-Protein and RBD of SARS-CoV-2 Do Not Bind and Hydrolyze DNA and Are Not Autoimmune |
| title_fullStr | Natural IgG against S-Protein and RBD of SARS-CoV-2 Do Not Bind and Hydrolyze DNA and Are Not Autoimmune |
| title_full_unstemmed | Natural IgG against S-Protein and RBD of SARS-CoV-2 Do Not Bind and Hydrolyze DNA and Are Not Autoimmune |
| title_short | Natural IgG against S-Protein and RBD of SARS-CoV-2 Do Not Bind and Hydrolyze DNA and Are Not Autoimmune |
| title_sort | natural igg against s protein and rbd of sars cov 2 do not bind and hydrolyze dna and are not autoimmune |
| topic | SARS-CoV-2 COVID-19 anti-dsDNA-IgGs autoimmunity spike protein RBD |
| url | https://www.mdpi.com/1422-0067/23/22/13681 |
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