Macrophage network dynamics depend on haptokinesis for optimal local surveillance
Macrophages are key immune cells with important roles for tissue surveillance in almost all mammalian organs. Cellular networks made up of many individual macrophages allow for optimal removal of dead cell material and pathogens in tissues. However, the critical determinants that underlie these popu...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2022-03-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/75354 |
| _version_ | 1811200937442672640 |
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| author | Neil Paterson Tim Lämmermann |
| author_facet | Neil Paterson Tim Lämmermann |
| author_sort | Neil Paterson |
| collection | DOAJ |
| description | Macrophages are key immune cells with important roles for tissue surveillance in almost all mammalian organs. Cellular networks made up of many individual macrophages allow for optimal removal of dead cell material and pathogens in tissues. However, the critical determinants that underlie these population responses have not been systematically studied. Here, we investigated how cell shape and the motility of individual cells influences macrophage network responses in 3D culture settings and in mouse tissues. We show that surveying macrophage populations can tolerate lowered actomyosin contractility, but cannot easily compensate for a lack of integrin-mediated adhesion. Although integrins were dispensable for macrophage chemotactic responses, they were crucial to control cell movement and protrusiveness for optimal surveillance by a macrophage population. Our study reveals that β1 integrins are important for maintaining macrophage shape and network sampling efficiency in mammalian tissues, and sets macrophage motility strategies apart from the integrin-independent 3D migration modes of many other immune cell subsets. |
| first_indexed | 2024-04-12T02:12:34Z |
| format | Article |
| id | doaj.art-805da628f37947db9af90dc059ade3d6 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T02:12:34Z |
| publishDate | 2022-03-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-805da628f37947db9af90dc059ade3d62022-12-22T03:52:20ZengeLife Sciences Publications LtdeLife2050-084X2022-03-011110.7554/eLife.75354Macrophage network dynamics depend on haptokinesis for optimal local surveillanceNeil Paterson0https://orcid.org/0000-0001-9563-5874Tim Lämmermann1https://orcid.org/0000-0002-8553-118XMax Planck Institute of Immunobiology and Epigenetics, Freiburg, Germany; International Max Planck Research School for Immunobiology, Epigenetics and Metabolism (IMPRS-IEM), Freiburg, Germany; Faculty of Biology, University of Freiburg, Freiburg, GermanyMax Planck Institute of Immunobiology and Epigenetics, Freiburg, GermanyMacrophages are key immune cells with important roles for tissue surveillance in almost all mammalian organs. Cellular networks made up of many individual macrophages allow for optimal removal of dead cell material and pathogens in tissues. However, the critical determinants that underlie these population responses have not been systematically studied. Here, we investigated how cell shape and the motility of individual cells influences macrophage network responses in 3D culture settings and in mouse tissues. We show that surveying macrophage populations can tolerate lowered actomyosin contractility, but cannot easily compensate for a lack of integrin-mediated adhesion. Although integrins were dispensable for macrophage chemotactic responses, they were crucial to control cell movement and protrusiveness for optimal surveillance by a macrophage population. Our study reveals that β1 integrins are important for maintaining macrophage shape and network sampling efficiency in mammalian tissues, and sets macrophage motility strategies apart from the integrin-independent 3D migration modes of many other immune cell subsets.https://elifesciences.org/articles/75354macrophagescell migrationintegrinstissue surveillanceefferocytosisimmune cells |
| spellingShingle | Neil Paterson Tim Lämmermann Macrophage network dynamics depend on haptokinesis for optimal local surveillance eLife macrophages cell migration integrins tissue surveillance efferocytosis immune cells |
| title | Macrophage network dynamics depend on haptokinesis for optimal local surveillance |
| title_full | Macrophage network dynamics depend on haptokinesis for optimal local surveillance |
| title_fullStr | Macrophage network dynamics depend on haptokinesis for optimal local surveillance |
| title_full_unstemmed | Macrophage network dynamics depend on haptokinesis for optimal local surveillance |
| title_short | Macrophage network dynamics depend on haptokinesis for optimal local surveillance |
| title_sort | macrophage network dynamics depend on haptokinesis for optimal local surveillance |
| topic | macrophages cell migration integrins tissue surveillance efferocytosis immune cells |
| url | https://elifesciences.org/articles/75354 |
| work_keys_str_mv | AT neilpaterson macrophagenetworkdynamicsdependonhaptokinesisforoptimallocalsurveillance AT timlammermann macrophagenetworkdynamicsdependonhaptokinesisforoptimallocalsurveillance |