In Vitro Antidiabetic and Antioxidant Effects of Different Extracts of <i>Catharanthus roseus</i> and Its Indole Alkaloid, Vindoline
The <i>Catharanthus roseus</i> plant has been used traditionally to treat diabetes mellitus. Scientific evidence supporting the antidiabetic effects of this plant’s active ingredient-vindoline has not been fully evaluated. In this study, extracts of <i>C. roseus</i> and vindo...
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MDPI AG
2020-11-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/25/23/5546 |
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| author | Mediline Goboza Mervin Meyer Yapo G. Aboua Oluwafemi O. Oguntibeju |
| author_facet | Mediline Goboza Mervin Meyer Yapo G. Aboua Oluwafemi O. Oguntibeju |
| author_sort | Mediline Goboza |
| collection | DOAJ |
| description | The <i>Catharanthus roseus</i> plant has been used traditionally to treat diabetes mellitus. Scientific evidence supporting the antidiabetic effects of this plant’s active ingredient-vindoline has not been fully evaluated. In this study, extracts of <i>C. roseus</i> and vindoline were tested for antioxidant activities, alpha amylase and alpha glucosidase inhibitory activities and insulin secretory effects in pancreatic RIN-5F cell line cultured in the absence of glucose, at low and high glucose concentrations. The methanolic extract of the plant showed the highest antioxidant activities in addition to the high total polyphenolic content (<i>p</i> < 0.05). The HPLC results exhibited increased concentration of vindoline in the dichloromethane and the ethylacetate extracts. Vindoline showed noticeable antioxidant activity when compared to ascorbic acid at <i>p</i> < 0.05 and significantly improved the in vitro insulin secretion. The intracellular reactive oxygen species formation in glucotoxicity-induced cells was significantly reduced following treatment with vindoline, methanolic and the dichloromethane extracts when compared to the high glucose untreated control (<i>p</i> < 0.05). Plant extracts and vindoline showed weaker inhibitory effects on the activities of carbohydrate metabolizing enzymes when compared to acarbose, which inhibited the activities of the enzymes by 80%. The plant extracts also exhibited weak alpha amylase and alpha glucosidase inhibitory effects. |
| first_indexed | 2024-03-10T14:33:06Z |
| format | Article |
| id | doaj.art-80601058a5c5426e87ff3d6186e4f2d4 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T14:33:06Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-80601058a5c5426e87ff3d6186e4f2d42023-11-20T22:26:12ZengMDPI AGMolecules1420-30492020-11-012523554610.3390/molecules25235546In Vitro Antidiabetic and Antioxidant Effects of Different Extracts of <i>Catharanthus roseus</i> and Its Indole Alkaloid, Vindoline Mediline Goboza0Mervin Meyer1Yapo G. Aboua2Oluwafemi O. Oguntibeju3Phytomedicine and Phytochemistry Group, Oxidative Stress Research Centre, Department of Biomedical Sciences, Faculty of Health & Wellness Sciences, Cape Peninsula University of Technology, Bellville 7535, South AfricaDSI/Mintek Nanotechnology Innovation Centre, Biolabels Node, Department of Biotechnology, University of the Western Cape, Bellville 7530, South AfricaDepartment of Health Sciences, Faculty of Health and Applied Sciences, Namibia University of Science and Technology, Windhoek 10005, NamibiaPhytomedicine and Phytochemistry Group, Oxidative Stress Research Centre, Department of Biomedical Sciences, Faculty of Health & Wellness Sciences, Cape Peninsula University of Technology, Bellville 7535, South AfricaThe <i>Catharanthus roseus</i> plant has been used traditionally to treat diabetes mellitus. Scientific evidence supporting the antidiabetic effects of this plant’s active ingredient-vindoline has not been fully evaluated. In this study, extracts of <i>C. roseus</i> and vindoline were tested for antioxidant activities, alpha amylase and alpha glucosidase inhibitory activities and insulin secretory effects in pancreatic RIN-5F cell line cultured in the absence of glucose, at low and high glucose concentrations. The methanolic extract of the plant showed the highest antioxidant activities in addition to the high total polyphenolic content (<i>p</i> < 0.05). The HPLC results exhibited increased concentration of vindoline in the dichloromethane and the ethylacetate extracts. Vindoline showed noticeable antioxidant activity when compared to ascorbic acid at <i>p</i> < 0.05 and significantly improved the in vitro insulin secretion. The intracellular reactive oxygen species formation in glucotoxicity-induced cells was significantly reduced following treatment with vindoline, methanolic and the dichloromethane extracts when compared to the high glucose untreated control (<i>p</i> < 0.05). Plant extracts and vindoline showed weaker inhibitory effects on the activities of carbohydrate metabolizing enzymes when compared to acarbose, which inhibited the activities of the enzymes by 80%. The plant extracts also exhibited weak alpha amylase and alpha glucosidase inhibitory effects.https://www.mdpi.com/1420-3049/25/23/5546insulin secretionglucotoxicityreactive oxygen speciesantioxidantbeta cellsalpha amylase |
| spellingShingle | Mediline Goboza Mervin Meyer Yapo G. Aboua Oluwafemi O. Oguntibeju In Vitro Antidiabetic and Antioxidant Effects of Different Extracts of <i>Catharanthus roseus</i> and Its Indole Alkaloid, Vindoline Molecules insulin secretion glucotoxicity reactive oxygen species antioxidant beta cells alpha amylase |
| title | In Vitro Antidiabetic and Antioxidant Effects of Different Extracts of <i>Catharanthus roseus</i> and Its Indole Alkaloid, Vindoline |
| title_full | In Vitro Antidiabetic and Antioxidant Effects of Different Extracts of <i>Catharanthus roseus</i> and Its Indole Alkaloid, Vindoline |
| title_fullStr | In Vitro Antidiabetic and Antioxidant Effects of Different Extracts of <i>Catharanthus roseus</i> and Its Indole Alkaloid, Vindoline |
| title_full_unstemmed | In Vitro Antidiabetic and Antioxidant Effects of Different Extracts of <i>Catharanthus roseus</i> and Its Indole Alkaloid, Vindoline |
| title_short | In Vitro Antidiabetic and Antioxidant Effects of Different Extracts of <i>Catharanthus roseus</i> and Its Indole Alkaloid, Vindoline |
| title_sort | in vitro antidiabetic and antioxidant effects of different extracts of i catharanthus roseus i and its indole alkaloid vindoline |
| topic | insulin secretion glucotoxicity reactive oxygen species antioxidant beta cells alpha amylase |
| url | https://www.mdpi.com/1420-3049/25/23/5546 |
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