Potentiating antimicrobial efficacy of propolis through niosomal-based system for administration
Background: Propolis is a multicomponent active, complex resinous substance collected by honeybees (Apis mellifera) from a variety of plant sources. This study was designed to improve the antimicrobial efficacy of propolis by engineering a niosomal-based system for topical application. Methods: Prop...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2015-06-01
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| Series: | Integrative Medicine Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213422014000742 |
| _version_ | 1819126985314533376 |
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| author | Jay Patel Sameer Ketkar Sharvil Patil James Fearnley Kakasaheb R. Mahadik Anant R. Paradkar |
| author_facet | Jay Patel Sameer Ketkar Sharvil Patil James Fearnley Kakasaheb R. Mahadik Anant R. Paradkar |
| author_sort | Jay Patel |
| collection | DOAJ |
| description | Background: Propolis is a multicomponent active, complex resinous substance collected by honeybees (Apis mellifera) from a variety of plant sources. This study was designed to improve the antimicrobial efficacy of propolis by engineering a niosomal-based system for topical application.
Methods: Propolis was extracted in ethanol and screened for total polyphenol content. Propolis-loaded niosomes (PLNs) were prepared with varying concentrations of Span 60 and cholesterol. The PLNs were evaluated for physicochemical parameters, namely, vesicle size, entrapment efficiency, zeta potential, surface topography and shape, and stability, followed by screening for in vitro antimicrobial activity. The PLNs were formulated into propolis niosomal gel (PNG) using Carbopol P934 base and subjected to ex vivo skin deposition study.
Results: The ethanolic extract of propolis had high polyphenolic content (270 ± 9.2 mg GAE/g). The prepared PLNs showed vesicle size between 294 nm and 427 nm, and the percent entrapment in the range of 50.62–71.29% with a significant enhancement in antimicrobial activity against Staphylococcus aureus and Candida albicans. Enhanced antimicrobial activity of PLNs was attributed to the ability of niosomes to directly interact with the bacterial cell envelop thereby facilitating the diffusion of propolis constituents across the cell wall. The formulated PNG exhibited a twofold better skin deposition due to improved retention of niosomes in the skin.
Conclusion: The findings indicate that the engineering of a niosomal-based system for propolis enhanced its antimicrobial potential through topical application. |
| first_indexed | 2024-12-22T08:04:44Z |
| format | Article |
| id | doaj.art-8060a3e10e314783a3c521c8f23be103 |
| institution | Directory Open Access Journal |
| issn | 2213-4220 |
| language | English |
| last_indexed | 2024-12-22T08:04:44Z |
| publishDate | 2015-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Integrative Medicine Research |
| spelling | doaj.art-8060a3e10e314783a3c521c8f23be1032022-12-21T18:33:10ZengElsevierIntegrative Medicine Research2213-42202015-06-01429410110.1016/j.imr.2014.10.004Potentiating antimicrobial efficacy of propolis through niosomal-based system for administrationJay Patel0Sameer Ketkar1Sharvil Patil2James Fearnley3Kakasaheb R. Mahadik4Anant R. Paradkar5Centre for Advanced Research in Pharmaceutical Sciences, Poona College of Pharmacy, Bharati Vidyapeeth University, Erandwane, IndiaCentre for Advanced Research in Pharmaceutical Sciences, Poona College of Pharmacy, Bharati Vidyapeeth University, Erandwane, IndiaCentre for Advanced Research in Pharmaceutical Sciences, Poona College of Pharmacy, Bharati Vidyapeeth University, Erandwane, IndiaApiceutical Research Centre, Whitby, United KingdomCentre for Advanced Research in Pharmaceutical Sciences, Poona College of Pharmacy, Bharati Vidyapeeth University, Erandwane, IndiaCentre for Pharmaceutical Engineering Sciences, University of Bradford, Bradford, United KingdomBackground: Propolis is a multicomponent active, complex resinous substance collected by honeybees (Apis mellifera) from a variety of plant sources. This study was designed to improve the antimicrobial efficacy of propolis by engineering a niosomal-based system for topical application. Methods: Propolis was extracted in ethanol and screened for total polyphenol content. Propolis-loaded niosomes (PLNs) were prepared with varying concentrations of Span 60 and cholesterol. The PLNs were evaluated for physicochemical parameters, namely, vesicle size, entrapment efficiency, zeta potential, surface topography and shape, and stability, followed by screening for in vitro antimicrobial activity. The PLNs were formulated into propolis niosomal gel (PNG) using Carbopol P934 base and subjected to ex vivo skin deposition study. Results: The ethanolic extract of propolis had high polyphenolic content (270 ± 9.2 mg GAE/g). The prepared PLNs showed vesicle size between 294 nm and 427 nm, and the percent entrapment in the range of 50.62–71.29% with a significant enhancement in antimicrobial activity against Staphylococcus aureus and Candida albicans. Enhanced antimicrobial activity of PLNs was attributed to the ability of niosomes to directly interact with the bacterial cell envelop thereby facilitating the diffusion of propolis constituents across the cell wall. The formulated PNG exhibited a twofold better skin deposition due to improved retention of niosomes in the skin. Conclusion: The findings indicate that the engineering of a niosomal-based system for propolis enhanced its antimicrobial potential through topical application.http://www.sciencedirect.com/science/article/pii/S2213422014000742antimicrobialniosomesnonionic surfactantspropolis |
| spellingShingle | Jay Patel Sameer Ketkar Sharvil Patil James Fearnley Kakasaheb R. Mahadik Anant R. Paradkar Potentiating antimicrobial efficacy of propolis through niosomal-based system for administration Integrative Medicine Research antimicrobial niosomes nonionic surfactants propolis |
| title | Potentiating antimicrobial efficacy of propolis through niosomal-based system for administration |
| title_full | Potentiating antimicrobial efficacy of propolis through niosomal-based system for administration |
| title_fullStr | Potentiating antimicrobial efficacy of propolis through niosomal-based system for administration |
| title_full_unstemmed | Potentiating antimicrobial efficacy of propolis through niosomal-based system for administration |
| title_short | Potentiating antimicrobial efficacy of propolis through niosomal-based system for administration |
| title_sort | potentiating antimicrobial efficacy of propolis through niosomal based system for administration |
| topic | antimicrobial niosomes nonionic surfactants propolis |
| url | http://www.sciencedirect.com/science/article/pii/S2213422014000742 |
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