MicroRNA-802 Suppresses Tumorigenesis of Colorectal Cancer via Regulating UBN2
Tao Yang,1 Qiuying Guo,2 Dongsheng Li,1 Guang Bai,1 Hongzhi Sun,1 Wei Wang1 1Department of General Surgery, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou 121001, People’s Republic of China; 2Operating Room, The First Affiliated Hospital of Jinzhou Medical University,...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2020-11-01
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| Series: | Cancer Management and Research |
| Subjects: | |
| Online Access: | https://www.dovepress.com/microrna-802-suppresses-tumorigenesis-of-colorectal-cancer-via-regulat-peer-reviewed-article-CMAR |
| _version_ | 1819171877985189888 |
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| author | Yang T Guo Q Li D Bai G Sun H Wang W |
| author_facet | Yang T Guo Q Li D Bai G Sun H Wang W |
| author_sort | Yang T |
| collection | DOAJ |
| description | Tao Yang,1 Qiuying Guo,2 Dongsheng Li,1 Guang Bai,1 Hongzhi Sun,1 Wei Wang1 1Department of General Surgery, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou 121001, People’s Republic of China; 2Operating Room, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou 121001, People’s Republic of ChinaCorrespondence: Wei Wang Email wangweijzmu24@qq.comBackground: The initiation and progression of colorectal cancer (CRC) are a multistep complex process regulated by multiple factors. Previous evidence indicated that microRNA-802 (miR-802) participated in tumorigenesis of numerous solid cancers; however, the potential roles and underlying mechanisms of miR‑802 in CRC still need further exploration.Methods: Quantitative real-time PCR (qRT-PCR) was employed to evaluate miR-802 levels in human CRC tissues and cell lines. In vitro proliferation, apoptosis, migration and invasion assays, and in vivo subcutaneous mouse xenograft model were utilized to examine the effects of miR-802 on the malignant behaviors of CRC cells. Then, bioinformatics prediction, dual-luciferase reporter, qRT-PCR, and Western blot was conducted to confirm the down-stream target of miR-802.Results: MiR-802 was frequently down-regulated in CRC tissues and cells. Further analyses showed that the low expression of miR-802 in CRC tissues was significantly correlated with tumor progression and poor patients’ prognosis. Overexpression of miR-802 profoundly inhibited proliferation, migration and invasion but promoted apoptosis of CRC cells, by contrast, miR-802 silencing exhibited opposite effects in vitro. Further animal experiment demonstrated that miR-802 could suppress tumor growth via inhibiting the proliferation and promoting the apoptosis of CRC cells in vivo. Mechanistically, miR-802 functioned as a tumor suppressor through inhibiting the expression of Ubinuclein-2 (UBN2) on post-transcriptional level. Moreover, upregulation of UBN2 expression could reverse the biological effects of CRC cells induced by miR-802 overexpression.Conclusion: Our study demonstrates that miR-802 inhibits the proliferation, migration and invasion while promotes the apoptosis of CRC cells via directly suppressing UBN2 expression. These findings provide a promising biomarker and potential treatment target for CRC.Keywords: colorectal cancer, miR-802, UBN2, proliferation, migration, invasion |
| first_indexed | 2024-12-22T19:58:17Z |
| format | Article |
| id | doaj.art-80682955f9194f72a95b3f9135108ee3 |
| institution | Directory Open Access Journal |
| issn | 1179-1322 |
| language | English |
| last_indexed | 2024-12-22T19:58:17Z |
| publishDate | 2020-11-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Cancer Management and Research |
| spelling | doaj.art-80682955f9194f72a95b3f9135108ee32022-12-21T18:14:21ZengDove Medical PressCancer Management and Research1179-13222020-11-01Volume 12112191123058932MicroRNA-802 Suppresses Tumorigenesis of Colorectal Cancer via Regulating UBN2Yang TGuo QLi DBai GSun HWang WTao Yang,1 Qiuying Guo,2 Dongsheng Li,1 Guang Bai,1 Hongzhi Sun,1 Wei Wang1 1Department of General Surgery, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou 121001, People’s Republic of China; 2Operating Room, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou 121001, People’s Republic of ChinaCorrespondence: Wei Wang Email wangweijzmu24@qq.comBackground: The initiation and progression of colorectal cancer (CRC) are a multistep complex process regulated by multiple factors. Previous evidence indicated that microRNA-802 (miR-802) participated in tumorigenesis of numerous solid cancers; however, the potential roles and underlying mechanisms of miR‑802 in CRC still need further exploration.Methods: Quantitative real-time PCR (qRT-PCR) was employed to evaluate miR-802 levels in human CRC tissues and cell lines. In vitro proliferation, apoptosis, migration and invasion assays, and in vivo subcutaneous mouse xenograft model were utilized to examine the effects of miR-802 on the malignant behaviors of CRC cells. Then, bioinformatics prediction, dual-luciferase reporter, qRT-PCR, and Western blot was conducted to confirm the down-stream target of miR-802.Results: MiR-802 was frequently down-regulated in CRC tissues and cells. Further analyses showed that the low expression of miR-802 in CRC tissues was significantly correlated with tumor progression and poor patients’ prognosis. Overexpression of miR-802 profoundly inhibited proliferation, migration and invasion but promoted apoptosis of CRC cells, by contrast, miR-802 silencing exhibited opposite effects in vitro. Further animal experiment demonstrated that miR-802 could suppress tumor growth via inhibiting the proliferation and promoting the apoptosis of CRC cells in vivo. Mechanistically, miR-802 functioned as a tumor suppressor through inhibiting the expression of Ubinuclein-2 (UBN2) on post-transcriptional level. Moreover, upregulation of UBN2 expression could reverse the biological effects of CRC cells induced by miR-802 overexpression.Conclusion: Our study demonstrates that miR-802 inhibits the proliferation, migration and invasion while promotes the apoptosis of CRC cells via directly suppressing UBN2 expression. These findings provide a promising biomarker and potential treatment target for CRC.Keywords: colorectal cancer, miR-802, UBN2, proliferation, migration, invasionhttps://www.dovepress.com/microrna-802-suppresses-tumorigenesis-of-colorectal-cancer-via-regulat-peer-reviewed-article-CMARcolorectal cancermir-802ubn2proliferationmigrationinvasion |
| spellingShingle | Yang T Guo Q Li D Bai G Sun H Wang W MicroRNA-802 Suppresses Tumorigenesis of Colorectal Cancer via Regulating UBN2 Cancer Management and Research colorectal cancer mir-802 ubn2 proliferation migration invasion |
| title | MicroRNA-802 Suppresses Tumorigenesis of Colorectal Cancer via Regulating UBN2 |
| title_full | MicroRNA-802 Suppresses Tumorigenesis of Colorectal Cancer via Regulating UBN2 |
| title_fullStr | MicroRNA-802 Suppresses Tumorigenesis of Colorectal Cancer via Regulating UBN2 |
| title_full_unstemmed | MicroRNA-802 Suppresses Tumorigenesis of Colorectal Cancer via Regulating UBN2 |
| title_short | MicroRNA-802 Suppresses Tumorigenesis of Colorectal Cancer via Regulating UBN2 |
| title_sort | microrna 802 suppresses tumorigenesis of colorectal cancer via regulating ubn2 |
| topic | colorectal cancer mir-802 ubn2 proliferation migration invasion |
| url | https://www.dovepress.com/microrna-802-suppresses-tumorigenesis-of-colorectal-cancer-via-regulat-peer-reviewed-article-CMAR |
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