Aberrant expression of miR-214 is associated with obesity-induced insulin resistance as a biomarker and therapeutic
Abstract Background Insulin resistance (IR) in obesity is associated with the occurrence of metabolic and cardiovascular diseases. Dipepidyl peptidase 4 (DPP4) plays a pivotal role during the development of IR, and was found to be a target gene of microRNA-214 (miR-214) in our study. This study soug...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2020-02-01
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| Series: | Diagnostic Pathology |
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| Online Access: | http://link.springer.com/article/10.1186/s13000-019-0914-1 |
| _version_ | 1811299283664633856 |
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| author | Fangxiao Cheng Geheng Yuan Jiao He Yimin Shao Junqing Zhang Xiaohui Guo |
| author_facet | Fangxiao Cheng Geheng Yuan Jiao He Yimin Shao Junqing Zhang Xiaohui Guo |
| author_sort | Fangxiao Cheng |
| collection | DOAJ |
| description | Abstract Background Insulin resistance (IR) in obesity is associated with the occurrence of metabolic and cardiovascular diseases. Dipepidyl peptidase 4 (DPP4) plays a pivotal role during the development of IR, and was found to be a target gene of microRNA-214 (miR-214) in our study. This study sought to assess the expression and clinical value of miR-214 in obese patients with IR, and investigate its therapeutic potential in obese rats and adipocytes with IR. Methods Serum expression of miR-214 in obese patients with or without IR was estimated by quantitative real-time-PCR. A receiver operating characteristic curve was plotted to evaluate the diagnostic value of miR-214 in the patients. Obesity-induced IR animal and cell models were constructed, and the therapeutic ability of miR-214 was explored. Results Serum expression of miR-214 was decreased in obese patients compared with the healthy controls, and the lowest expression was observed in the cases with IR. Downregulation of miR-214 was significantly correlated with the serum DPP4 levels and HOMA-IR of the patients upon IR conditions, and was demonstrated to perform diagnostic accuracy for distinguishing obese patients with IR from those without IR. In obesity-associated IR animal and cell models, the downregulation of miR-214 was also been detected. According to the measurement of glucose and insulin tolerance and glucose uptake abilities, we found that the overexpression of miR-214 could be used to alleviate IR in the IR models, especially when collaboratively used with DPP4 inhibitor vildagliptin. Conclusion All data revealed that miR-214, as a regulator of DPP4, is decreased in obese patients with IR and may serve as a diagnostic biomarker. The upregulation of miR-214 could improve IR in obese rats and adipocytes, indicating that miR-214 has the therapeutic potential for obesity and IR. |
| first_indexed | 2024-04-13T06:33:48Z |
| format | Article |
| id | doaj.art-8069d894b2224d4a8abdaf1a1cd49d65 |
| institution | Directory Open Access Journal |
| issn | 1746-1596 |
| language | English |
| last_indexed | 2024-04-13T06:33:48Z |
| publishDate | 2020-02-01 |
| publisher | BMC |
| record_format | Article |
| series | Diagnostic Pathology |
| spelling | doaj.art-8069d894b2224d4a8abdaf1a1cd49d652022-12-22T02:58:01ZengBMCDiagnostic Pathology1746-15962020-02-0115111010.1186/s13000-019-0914-1Aberrant expression of miR-214 is associated with obesity-induced insulin resistance as a biomarker and therapeuticFangxiao Cheng0Geheng Yuan1Jiao He2Yimin Shao3Junqing Zhang4Xiaohui Guo5Department of Endocrinology, Peking University First HospitalDepartment of Endocrinology, Peking University First HospitalDepartment of Endocrinology, Baoding First Central HispitalDepartment of Endocrinology, Peking University First HospitalDepartment of Endocrinology, Peking University First HospitalDepartment of Endocrinology, Peking University First HospitalAbstract Background Insulin resistance (IR) in obesity is associated with the occurrence of metabolic and cardiovascular diseases. Dipepidyl peptidase 4 (DPP4) plays a pivotal role during the development of IR, and was found to be a target gene of microRNA-214 (miR-214) in our study. This study sought to assess the expression and clinical value of miR-214 in obese patients with IR, and investigate its therapeutic potential in obese rats and adipocytes with IR. Methods Serum expression of miR-214 in obese patients with or without IR was estimated by quantitative real-time-PCR. A receiver operating characteristic curve was plotted to evaluate the diagnostic value of miR-214 in the patients. Obesity-induced IR animal and cell models were constructed, and the therapeutic ability of miR-214 was explored. Results Serum expression of miR-214 was decreased in obese patients compared with the healthy controls, and the lowest expression was observed in the cases with IR. Downregulation of miR-214 was significantly correlated with the serum DPP4 levels and HOMA-IR of the patients upon IR conditions, and was demonstrated to perform diagnostic accuracy for distinguishing obese patients with IR from those without IR. In obesity-associated IR animal and cell models, the downregulation of miR-214 was also been detected. According to the measurement of glucose and insulin tolerance and glucose uptake abilities, we found that the overexpression of miR-214 could be used to alleviate IR in the IR models, especially when collaboratively used with DPP4 inhibitor vildagliptin. Conclusion All data revealed that miR-214, as a regulator of DPP4, is decreased in obese patients with IR and may serve as a diagnostic biomarker. The upregulation of miR-214 could improve IR in obese rats and adipocytes, indicating that miR-214 has the therapeutic potential for obesity and IR.http://link.springer.com/article/10.1186/s13000-019-0914-1ObesityInsulin resistanceMicroRNA-214DDP4Adipocyte |
| spellingShingle | Fangxiao Cheng Geheng Yuan Jiao He Yimin Shao Junqing Zhang Xiaohui Guo Aberrant expression of miR-214 is associated with obesity-induced insulin resistance as a biomarker and therapeutic Diagnostic Pathology Obesity Insulin resistance MicroRNA-214 DDP4 Adipocyte |
| title | Aberrant expression of miR-214 is associated with obesity-induced insulin resistance as a biomarker and therapeutic |
| title_full | Aberrant expression of miR-214 is associated with obesity-induced insulin resistance as a biomarker and therapeutic |
| title_fullStr | Aberrant expression of miR-214 is associated with obesity-induced insulin resistance as a biomarker and therapeutic |
| title_full_unstemmed | Aberrant expression of miR-214 is associated with obesity-induced insulin resistance as a biomarker and therapeutic |
| title_short | Aberrant expression of miR-214 is associated with obesity-induced insulin resistance as a biomarker and therapeutic |
| title_sort | aberrant expression of mir 214 is associated with obesity induced insulin resistance as a biomarker and therapeutic |
| topic | Obesity Insulin resistance MicroRNA-214 DDP4 Adipocyte |
| url | http://link.springer.com/article/10.1186/s13000-019-0914-1 |
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