Gene expression profiles of skin from cyclin dependent kinases 5-knockdown mice
Objective This study aimed to identify genes regulated by cyclin dependent kinases 5 (CDK5) that participate in hair pigmentation in mice. Methods The mRNA expression profiles of skin samples from CDK5-knockdown mice were constructed using high-throughput RNA sequencing and compared with those of wi...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Asian-Australasian Association of Animal Production Societies
2024-04-01
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| Series: | Animal Bioscience |
| Subjects: | |
| Online Access: | http://www.animbiosci.org/upload/pdf/ab-23-0244.pdf |
| _version_ | 1827283872787726336 |
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| author | Shanshan Yang Dingxing Jiao Tao Song Ping Rui Ruiwen Fan Zengjun Ma |
| author_facet | Shanshan Yang Dingxing Jiao Tao Song Ping Rui Ruiwen Fan Zengjun Ma |
| author_sort | Shanshan Yang |
| collection | DOAJ |
| description | Objective This study aimed to identify genes regulated by cyclin dependent kinases 5 (CDK5) that participate in hair pigmentation in mice. Methods The mRNA expression profiles of skin samples from CDK5-knockdown mice were constructed using high-throughput RNA sequencing and compared with those of wild-type mice. Results In total, 8,002 known genes were differentially expressed between CDK5-knockdown and wild-type mice. Of these, 3,658 were upregulated and 4,344 were downregulated in the skin of CDK5-knockdown mice. An additional 318 previously unknown genes were also differentially expressed, with 171 downregulated and 147 upregulated genes in the skin of CDK5-knockdown mice. Of the known genes expressed in mouse skin, 80 were associated with hair color, with 61 showing lower expression and 19 exhibiting higher expression in skin of CDK5-knockdown mice. Importantly, the expression of the tyrosinase-related protein 1 (TYRP1) and the calcium signaling pathway were also found to be regulated by CDK5, suggesting that pigmentation is regulated by CDK5 via the calcium signaling pathway and TYRP1. Conclusion The transcriptome profiles obtained from the skin of CDK5-knockdown mice compared to wild-type mice provide a valuable resource to help understand the mechanism by which CDK5 regulates melanogenesis in mice and other animals. |
| first_indexed | 2024-03-07T14:30:10Z |
| format | Article |
| id | doaj.art-806c10a87a514bbbb335469ef6e2a000 |
| institution | Directory Open Access Journal |
| issn | 2765-0189 2765-0235 |
| language | English |
| last_indexed | 2024-04-24T09:44:30Z |
| publishDate | 2024-04-01 |
| publisher | Asian-Australasian Association of Animal Production Societies |
| record_format | Article |
| series | Animal Bioscience |
| spelling | doaj.art-806c10a87a514bbbb335469ef6e2a0002024-04-15T04:05:41ZengAsian-Australasian Association of Animal Production SocietiesAnimal Bioscience2765-01892765-02352024-04-0137456757510.5713/ab.23.024425195Gene expression profiles of skin from cyclin dependent kinases 5-knockdown miceShanshan Yang0Dingxing Jiao1Tao Song2Ping Rui3Ruiwen Fan4Zengjun Ma5 College of Animal Science and Technology, Hebei Normal University of Science & Technology, Qinhuangdao 066600, China College of Animal Science and Technology, Hebei Normal University of Science & Technology, Qinhuangdao 066600, China College of Animal Science and Technology, Hebei Normal University of Science & Technology, Qinhuangdao 066600, China College of Animal Science and Technology, Hebei Normal University of Science & Technology, Qinhuangdao 066600, China College of Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi 030801, China College of Animal Science and Technology, Hebei Normal University of Science & Technology, Qinhuangdao 066600, ChinaObjective This study aimed to identify genes regulated by cyclin dependent kinases 5 (CDK5) that participate in hair pigmentation in mice. Methods The mRNA expression profiles of skin samples from CDK5-knockdown mice were constructed using high-throughput RNA sequencing and compared with those of wild-type mice. Results In total, 8,002 known genes were differentially expressed between CDK5-knockdown and wild-type mice. Of these, 3,658 were upregulated and 4,344 were downregulated in the skin of CDK5-knockdown mice. An additional 318 previously unknown genes were also differentially expressed, with 171 downregulated and 147 upregulated genes in the skin of CDK5-knockdown mice. Of the known genes expressed in mouse skin, 80 were associated with hair color, with 61 showing lower expression and 19 exhibiting higher expression in skin of CDK5-knockdown mice. Importantly, the expression of the tyrosinase-related protein 1 (TYRP1) and the calcium signaling pathway were also found to be regulated by CDK5, suggesting that pigmentation is regulated by CDK5 via the calcium signaling pathway and TYRP1. Conclusion The transcriptome profiles obtained from the skin of CDK5-knockdown mice compared to wild-type mice provide a valuable resource to help understand the mechanism by which CDK5 regulates melanogenesis in mice and other animals.http://www.animbiosci.org/upload/pdf/ab-23-0244.pdfcyclin dependent kinases 5 (cdk5)gene expression profilemelanogenesispigmentation |
| spellingShingle | Shanshan Yang Dingxing Jiao Tao Song Ping Rui Ruiwen Fan Zengjun Ma Gene expression profiles of skin from cyclin dependent kinases 5-knockdown mice Animal Bioscience cyclin dependent kinases 5 (cdk5) gene expression profile melanogenesis pigmentation |
| title | Gene expression profiles of skin from cyclin dependent kinases 5-knockdown mice |
| title_full | Gene expression profiles of skin from cyclin dependent kinases 5-knockdown mice |
| title_fullStr | Gene expression profiles of skin from cyclin dependent kinases 5-knockdown mice |
| title_full_unstemmed | Gene expression profiles of skin from cyclin dependent kinases 5-knockdown mice |
| title_short | Gene expression profiles of skin from cyclin dependent kinases 5-knockdown mice |
| title_sort | gene expression profiles of skin from cyclin dependent kinases 5 knockdown mice |
| topic | cyclin dependent kinases 5 (cdk5) gene expression profile melanogenesis pigmentation |
| url | http://www.animbiosci.org/upload/pdf/ab-23-0244.pdf |
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