Protective effects and potential mechanisms of fermented egg-milk peptides on the damaged intestinal barrier
IntroductionFermented egg-milk peptides (FEMPs) could enhance the colon-intestinal barrier and upgrade the expression of zonula occludens-1 and mucin 2. Besides, the underlying biological mechanism and the targets FEMPs could regulate were analyzed in our study.MethodsHerein, the immunofluorescence...
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| Format: | Article |
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Frontiers Media S.A.
2022-12-01
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| Series: | Frontiers in Nutrition |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnut.2022.1068877/full |
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| author | Siwen Lyu Qi Yang Xuehui Duan Xuanting Liu Zhiyang Du Xiaomin Shang Menglei Xu Jingbo Liu Fengguang Pan Ting Zhang |
| author_facet | Siwen Lyu Qi Yang Xuehui Duan Xuanting Liu Zhiyang Du Xiaomin Shang Menglei Xu Jingbo Liu Fengguang Pan Ting Zhang |
| author_sort | Siwen Lyu |
| collection | DOAJ |
| description | IntroductionFermented egg-milk peptides (FEMPs) could enhance the colon-intestinal barrier and upgrade the expression of zonula occludens-1 and mucin 2. Besides, the underlying biological mechanism and the targets FEMPs could regulate were analyzed in our study.MethodsHerein, the immunofluorescence technique and western blot were utilized to evaluate the repair of the intestinal barrier. Network pharmacology analysis and bioinformatics methods were performed to investigate the targets and pathways affected by FEMPs.Results and discussionAnimal experiments showed that FEMPs could restore intestinal damage and enhance the expression of two key proteins. The pharmacological results revealed that FEMPs could regulate targets related to kinase activity, such as AKT, CASP, RAF, and GSK. The above targets could interact with each other. GO analysis indicated that the targets regulated by FEMPs could participate in the kinase activity of the metabolic process. KEGG enrichment revealed that the core targets were enriched in pathways related to cell apoptosis and other important procedures. Molecular docking demonstrated that FEMPs could bind to the key target AKT via hydrogen bond interactions. Our study combined the experiment in vivo with the method in silico and investigated the interaction between peptides and targets in a pattern of multi-targets and multi-pathways, which offered a new perspective on the functional validation and potential application of bioactive peptides. |
| first_indexed | 2024-04-11T13:27:57Z |
| format | Article |
| id | doaj.art-806c3a3464b248ecb67df8369ba54c06 |
| institution | Directory Open Access Journal |
| issn | 2296-861X |
| language | English |
| last_indexed | 2024-04-11T13:27:57Z |
| publishDate | 2022-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Nutrition |
| spelling | doaj.art-806c3a3464b248ecb67df8369ba54c062022-12-22T04:21:58ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2022-12-01910.3389/fnut.2022.10688771068877Protective effects and potential mechanisms of fermented egg-milk peptides on the damaged intestinal barrierSiwen Lyu0Qi Yang1Xuehui Duan2Xuanting Liu3Zhiyang Du4Xiaomin Shang5Menglei Xu6Jingbo Liu7Fengguang Pan8Ting Zhang9Jilin Provincial Key Laboratory of Nutrition and Functional Food, College of Food Science and Engineering, Jilin University, Changchun, ChinaJilin Provincial Key Laboratory of Nutrition and Functional Food, College of Food Science and Engineering, Jilin University, Changchun, ChinaJilin Provincial Key Laboratory of Nutrition and Functional Food, College of Food Science and Engineering, Jilin University, Changchun, ChinaJilin Provincial Key Laboratory of Nutrition and Functional Food, College of Food Science and Engineering, Jilin University, Changchun, ChinaJilin Provincial Key Laboratory of Nutrition and Functional Food, College of Food Science and Engineering, Jilin University, Changchun, ChinaJilin Provincial Key Laboratory of Nutrition and Functional Food, College of Food Science and Engineering, Jilin University, Changchun, ChinaState Key Laboratory of Supramolecular Structure and Materials, Jilin University, Changchun, ChinaJilin Provincial Key Laboratory of Nutrition and Functional Food, College of Food Science and Engineering, Jilin University, Changchun, ChinaJilin Provincial Key Laboratory of Nutrition and Functional Food, College of Food Science and Engineering, Jilin University, Changchun, ChinaJilin Provincial Key Laboratory of Nutrition and Functional Food, College of Food Science and Engineering, Jilin University, Changchun, ChinaIntroductionFermented egg-milk peptides (FEMPs) could enhance the colon-intestinal barrier and upgrade the expression of zonula occludens-1 and mucin 2. Besides, the underlying biological mechanism and the targets FEMPs could regulate were analyzed in our study.MethodsHerein, the immunofluorescence technique and western blot were utilized to evaluate the repair of the intestinal barrier. Network pharmacology analysis and bioinformatics methods were performed to investigate the targets and pathways affected by FEMPs.Results and discussionAnimal experiments showed that FEMPs could restore intestinal damage and enhance the expression of two key proteins. The pharmacological results revealed that FEMPs could regulate targets related to kinase activity, such as AKT, CASP, RAF, and GSK. The above targets could interact with each other. GO analysis indicated that the targets regulated by FEMPs could participate in the kinase activity of the metabolic process. KEGG enrichment revealed that the core targets were enriched in pathways related to cell apoptosis and other important procedures. Molecular docking demonstrated that FEMPs could bind to the key target AKT via hydrogen bond interactions. Our study combined the experiment in vivo with the method in silico and investigated the interaction between peptides and targets in a pattern of multi-targets and multi-pathways, which offered a new perspective on the functional validation and potential application of bioactive peptides.https://www.frontiersin.org/articles/10.3389/fnut.2022.1068877/fullpeptidesintestinal barrierzonula occludens-1mucinbioinformatics |
| spellingShingle | Siwen Lyu Qi Yang Xuehui Duan Xuanting Liu Zhiyang Du Xiaomin Shang Menglei Xu Jingbo Liu Fengguang Pan Ting Zhang Protective effects and potential mechanisms of fermented egg-milk peptides on the damaged intestinal barrier Frontiers in Nutrition peptides intestinal barrier zonula occludens-1 mucin bioinformatics |
| title | Protective effects and potential mechanisms of fermented egg-milk peptides on the damaged intestinal barrier |
| title_full | Protective effects and potential mechanisms of fermented egg-milk peptides on the damaged intestinal barrier |
| title_fullStr | Protective effects and potential mechanisms of fermented egg-milk peptides on the damaged intestinal barrier |
| title_full_unstemmed | Protective effects and potential mechanisms of fermented egg-milk peptides on the damaged intestinal barrier |
| title_short | Protective effects and potential mechanisms of fermented egg-milk peptides on the damaged intestinal barrier |
| title_sort | protective effects and potential mechanisms of fermented egg milk peptides on the damaged intestinal barrier |
| topic | peptides intestinal barrier zonula occludens-1 mucin bioinformatics |
| url | https://www.frontiersin.org/articles/10.3389/fnut.2022.1068877/full |
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