Metabolic Features of a Novel <i>Trichoderma asperellum</i> YNQJ1002 with Potent Antagonistic Activity against <i>Fusarium graminearum</i>
<i>Trichoderma</i>, a well-known and extensively studied fungal genus, has gained significant attention for its remarkable antagonistic abilities against a wide range of plant pathogens. In this study, a total of 108 <i>Trichoderma</i> isolates were screened through in vitro...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-11-01
|
| Series: | Metabolites |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2218-1989/13/11/1144 |
| _version_ | 1827639275605196800 |
|---|---|
| author | Huimin Ji Ruohan Yu Hongyi Liu Hui Zhang Xinhua Wang Jie Chen Yaqian Li |
| author_facet | Huimin Ji Ruohan Yu Hongyi Liu Hui Zhang Xinhua Wang Jie Chen Yaqian Li |
| author_sort | Huimin Ji |
| collection | DOAJ |
| description | <i>Trichoderma</i>, a well-known and extensively studied fungal genus, has gained significant attention for its remarkable antagonistic abilities against a wide range of plant pathogens. In this study, a total of 108 <i>Trichoderma</i> isolates were screened through in vitro dual antagonistic assays and culture filtrate inhibition against <i>Fusarium graminearum</i>. Of these, the YNQJ1002 displayed noteworthy inhibitory activities along with thermal stability. To validate the metabolic differences between YNQJ1002 and GZLX3001 (with strong and weak antagonism, respectively), UPLC-TOF-MS/MS mass spectrometry was employed to analyze and compare the metabolite profiles. We identified 12 significantly up-regulated metabolites in YNQJ1002, which include compounds like Trigoneoside, Torvoside, trans,trans-hepta-2,4,6-trienoic acid, and Chamazulene. These metabolites are known for their antimicrobial properties or signaling roles as components of cell membranes. Enriched KEGG analysis revealed a significant enrichment in sphingolipid metabolism and linoleic acid metabolism, as well as autophagy. The results demonstrated that YNQJ1002’s abundance of antimicrobial substances, resulting from specific metabolic pathways, enhanced its superior antagonistic activity against <i>F. graminearum</i>. Finally, YNQJ1002 was identified using the ITS, <i>tef1-1α</i>, and <i>rpb2</i> regions, with MIST system sequence matching confirming its classification within the species. Overall, we have obtained a novel strain, <i>T. asperellum</i> YNQJ1002, which is rich in metabolites and shows potential antagonistic activity against <i>F. graminearum.</i> This study has opened promising prospects for the development of innovative <i>Trichoderma</i>-derived antifungal compounds, featuring a unique mechanism against pathogens. |
| first_indexed | 2024-03-09T16:37:16Z |
| format | Article |
| id | doaj.art-806d95387c8b4dfc9c523846d5d3e085 |
| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-09T16:37:16Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-806d95387c8b4dfc9c523846d5d3e0852023-11-24T14:55:29ZengMDPI AGMetabolites2218-19892023-11-011311114410.3390/metabo13111144Metabolic Features of a Novel <i>Trichoderma asperellum</i> YNQJ1002 with Potent Antagonistic Activity against <i>Fusarium graminearum</i>Huimin Ji0Ruohan Yu1Hongyi Liu2Hui Zhang3Xinhua Wang4Jie Chen5Yaqian Li6School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaSchool of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaSchool of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaSchool of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaSchool of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaSchool of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaSchool of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China<i>Trichoderma</i>, a well-known and extensively studied fungal genus, has gained significant attention for its remarkable antagonistic abilities against a wide range of plant pathogens. In this study, a total of 108 <i>Trichoderma</i> isolates were screened through in vitro dual antagonistic assays and culture filtrate inhibition against <i>Fusarium graminearum</i>. Of these, the YNQJ1002 displayed noteworthy inhibitory activities along with thermal stability. To validate the metabolic differences between YNQJ1002 and GZLX3001 (with strong and weak antagonism, respectively), UPLC-TOF-MS/MS mass spectrometry was employed to analyze and compare the metabolite profiles. We identified 12 significantly up-regulated metabolites in YNQJ1002, which include compounds like Trigoneoside, Torvoside, trans,trans-hepta-2,4,6-trienoic acid, and Chamazulene. These metabolites are known for their antimicrobial properties or signaling roles as components of cell membranes. Enriched KEGG analysis revealed a significant enrichment in sphingolipid metabolism and linoleic acid metabolism, as well as autophagy. The results demonstrated that YNQJ1002’s abundance of antimicrobial substances, resulting from specific metabolic pathways, enhanced its superior antagonistic activity against <i>F. graminearum</i>. Finally, YNQJ1002 was identified using the ITS, <i>tef1-1α</i>, and <i>rpb2</i> regions, with MIST system sequence matching confirming its classification within the species. Overall, we have obtained a novel strain, <i>T. asperellum</i> YNQJ1002, which is rich in metabolites and shows potential antagonistic activity against <i>F. graminearum.</i> This study has opened promising prospects for the development of innovative <i>Trichoderma</i>-derived antifungal compounds, featuring a unique mechanism against pathogens.https://www.mdpi.com/2218-1989/13/11/1144antagonistic activityfusariummetabolomic comparative analysis<i>Trichoderma asperellum</i>UPLC-MS/MS |
| spellingShingle | Huimin Ji Ruohan Yu Hongyi Liu Hui Zhang Xinhua Wang Jie Chen Yaqian Li Metabolic Features of a Novel <i>Trichoderma asperellum</i> YNQJ1002 with Potent Antagonistic Activity against <i>Fusarium graminearum</i> Metabolites antagonistic activity fusarium metabolomic comparative analysis <i>Trichoderma asperellum</i> UPLC-MS/MS |
| title | Metabolic Features of a Novel <i>Trichoderma asperellum</i> YNQJ1002 with Potent Antagonistic Activity against <i>Fusarium graminearum</i> |
| title_full | Metabolic Features of a Novel <i>Trichoderma asperellum</i> YNQJ1002 with Potent Antagonistic Activity against <i>Fusarium graminearum</i> |
| title_fullStr | Metabolic Features of a Novel <i>Trichoderma asperellum</i> YNQJ1002 with Potent Antagonistic Activity against <i>Fusarium graminearum</i> |
| title_full_unstemmed | Metabolic Features of a Novel <i>Trichoderma asperellum</i> YNQJ1002 with Potent Antagonistic Activity against <i>Fusarium graminearum</i> |
| title_short | Metabolic Features of a Novel <i>Trichoderma asperellum</i> YNQJ1002 with Potent Antagonistic Activity against <i>Fusarium graminearum</i> |
| title_sort | metabolic features of a novel i trichoderma asperellum i ynqj1002 with potent antagonistic activity against i fusarium graminearum i |
| topic | antagonistic activity fusarium metabolomic comparative analysis <i>Trichoderma asperellum</i> UPLC-MS/MS |
| url | https://www.mdpi.com/2218-1989/13/11/1144 |
| work_keys_str_mv | AT huiminji metabolicfeaturesofanovelitrichodermaasperellumiynqj1002withpotentantagonisticactivityagainstifusariumgraminearumi AT ruohanyu metabolicfeaturesofanovelitrichodermaasperellumiynqj1002withpotentantagonisticactivityagainstifusariumgraminearumi AT hongyiliu metabolicfeaturesofanovelitrichodermaasperellumiynqj1002withpotentantagonisticactivityagainstifusariumgraminearumi AT huizhang metabolicfeaturesofanovelitrichodermaasperellumiynqj1002withpotentantagonisticactivityagainstifusariumgraminearumi AT xinhuawang metabolicfeaturesofanovelitrichodermaasperellumiynqj1002withpotentantagonisticactivityagainstifusariumgraminearumi AT jiechen metabolicfeaturesofanovelitrichodermaasperellumiynqj1002withpotentantagonisticactivityagainstifusariumgraminearumi AT yaqianli metabolicfeaturesofanovelitrichodermaasperellumiynqj1002withpotentantagonisticactivityagainstifusariumgraminearumi |