Identification of Arisaema Species by leaf arrangement and molecular markers
The objectives of this study were to identify three Arisaema amurense, A. ringens and A. triphyllum specious based on molecular markers and to select accessions with unique morphological characters. Tentative identification based on leaf morphology was verified using single nucleotide polymorphis...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Academy of Agricultural and Forestry Sciences
2022-12-01
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| Series: | Romanian Journal of Horticulture |
| Subjects: | |
| Online Access: | https://romanianjournalofhorticulture.ro/wp-content/uploads/2022/12/01.-9-16.pdf |
| Summary: | The objectives of this study were to identify three Arisaema amurense, A.
ringens and A. triphyllum specious based on molecular markers and to select
accessions with unique morphological characters. Tentative identification based on
leaf morphology was verified using single nucleotide polymorphisms (SNPs)
generated from sequence analysis of the nuclear transcribed spacer of rDNA gene
(nrITS) and the chloroplast intergenic spacer of trnL-trnF gene (cpIS). Specific SNPs
from nrITS for A. amurense and A. triphyllum were identified. However, A. ringensspecific SNPs could not be identified because of multiple variations in the nrITS
sequences of the species. Species-specific SNPs for the conserved cpIS sequenced in
the same species were identified for each species. Morphological characteristics
based on the number of leaflets were not sufficient to characterize closely related
species such as A. amurense and A. ringens. Arisaema amurense with a unique leaf
morphology with variegated, serrated, and denticulate foliage could be selected for
further evaluation as a source of new ornamental germplasm |
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| ISSN: | 2734-7656 2734-8083 |