Ectopic Expression of OsPYL/RCAR7, an ABA Receptor Having Low Signaling Activity, Improves Drought Tolerance without Growth Defects in Rice
Overexpression of abscisic acid (ABA) receptors has been reported to enhance drought tolerance, but also to cause stunted growth and decreased crop yield. Here, we constructed transgenic rice for all monomeric ABA receptors and observed that only transgenic rice over-expressing <i>OsPYL/RCAR7&...
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| Format: | Article |
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MDPI AG
2020-06-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/21/11/4163 |
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| author | Nikita Bhatnagar Rigyeong Kim Seungsu Han Jaeeun Song Gang Seob Lee Sangho Lee Myung Ki Min Beom-Gi Kim |
| author_facet | Nikita Bhatnagar Rigyeong Kim Seungsu Han Jaeeun Song Gang Seob Lee Sangho Lee Myung Ki Min Beom-Gi Kim |
| author_sort | Nikita Bhatnagar |
| collection | DOAJ |
| description | Overexpression of abscisic acid (ABA) receptors has been reported to enhance drought tolerance, but also to cause stunted growth and decreased crop yield. Here, we constructed transgenic rice for all monomeric ABA receptors and observed that only transgenic rice over-expressing <i>OsPYL/RCAR7</i> showed similar phenotype with wild type, without total yield loss when grown under normal growth condition in a paddy field. Even though transgenic rice over-expressing <i>OsPYL</i>/<i>RCAR7</i> showed neither an ABA-sensitivity nor an osmotic stress tolerance in plate assay, it showed drought tolerance. We investigated the ABA-dependent interaction with OsPP2CAs and ABA signaling induction by OsPYL/RCAR7. In yeast two hybrid assay, OsPYL/RCAR7 required critically higher ABA concentrations to interact with OsPP2CAs than other ABA receptors, and co-immunoprecipitation assay showed strong interaction under ABA treatment. When ABA-responsive signaling activity was monitored using a transient expression system in rice protoplasts, OsPYL/RCAR7 had the lowest ABA-responsive signaling activity as compared with other ABA receptors. OsPYL/RCAR7 also showed weak suppression of phosphatase activity as compared with other ABA receptors in vitro. Transcriptome analysis of transgenic rice over-expressing <i>OsPYL/RCAR7</i> suggested that only a few genes were induced similar to control under without exogenous ABA, but a large number of genes was induced under ABA treatment compared with control. We conclude that OsPYL/RCAR7 is a novel functional ABA receptor that has low ABA signaling activity and exhibits high ABA dependence. These results lay the foundation for a new strategy to improve drought stress tolerance without compromising crop growth. |
| first_indexed | 2024-03-10T19:15:07Z |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T19:15:07Z |
| publishDate | 2020-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-806f17d1d1724518b7536ff2718992a32023-11-20T03:30:05ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-06-012111416310.3390/ijms21114163Ectopic Expression of OsPYL/RCAR7, an ABA Receptor Having Low Signaling Activity, Improves Drought Tolerance without Growth Defects in RiceNikita Bhatnagar0Rigyeong Kim1Seungsu Han2Jaeeun Song3Gang Seob Lee4Sangho Lee5Myung Ki Min6Beom-Gi Kim7Metabolic Engineering Division, National Institute of Agricultural Sciences, RDA, Jeonju, Jeollabuk-do 54874, KoreaMetabolic Engineering Division, National Institute of Agricultural Sciences, RDA, Jeonju, Jeollabuk-do 54874, KoreaDepartment of Biological Sciences, Sungkyunkwan University, Suwon 16419, KoreaMetabolic Engineering Division, National Institute of Agricultural Sciences, RDA, Jeonju, Jeollabuk-do 54874, KoreaBiosafety Division, National Institute of Agricultural Sciences, RDA, Jeonju, Jeollabuk-do 54874, KoreaDepartment of Biological Sciences, Sungkyunkwan University, Suwon 16419, KoreaMetabolic Engineering Division, National Institute of Agricultural Sciences, RDA, Jeonju, Jeollabuk-do 54874, KoreaMetabolic Engineering Division, National Institute of Agricultural Sciences, RDA, Jeonju, Jeollabuk-do 54874, KoreaOverexpression of abscisic acid (ABA) receptors has been reported to enhance drought tolerance, but also to cause stunted growth and decreased crop yield. Here, we constructed transgenic rice for all monomeric ABA receptors and observed that only transgenic rice over-expressing <i>OsPYL/RCAR7</i> showed similar phenotype with wild type, without total yield loss when grown under normal growth condition in a paddy field. Even though transgenic rice over-expressing <i>OsPYL</i>/<i>RCAR7</i> showed neither an ABA-sensitivity nor an osmotic stress tolerance in plate assay, it showed drought tolerance. We investigated the ABA-dependent interaction with OsPP2CAs and ABA signaling induction by OsPYL/RCAR7. In yeast two hybrid assay, OsPYL/RCAR7 required critically higher ABA concentrations to interact with OsPP2CAs than other ABA receptors, and co-immunoprecipitation assay showed strong interaction under ABA treatment. When ABA-responsive signaling activity was monitored using a transient expression system in rice protoplasts, OsPYL/RCAR7 had the lowest ABA-responsive signaling activity as compared with other ABA receptors. OsPYL/RCAR7 also showed weak suppression of phosphatase activity as compared with other ABA receptors in vitro. Transcriptome analysis of transgenic rice over-expressing <i>OsPYL/RCAR7</i> suggested that only a few genes were induced similar to control under without exogenous ABA, but a large number of genes was induced under ABA treatment compared with control. We conclude that OsPYL/RCAR7 is a novel functional ABA receptor that has low ABA signaling activity and exhibits high ABA dependence. These results lay the foundation for a new strategy to improve drought stress tolerance without compromising crop growth.https://www.mdpi.com/1422-0067/21/11/4163abiotic stress signalingABA signalingcropgrowth and stress tolerance balance |
| spellingShingle | Nikita Bhatnagar Rigyeong Kim Seungsu Han Jaeeun Song Gang Seob Lee Sangho Lee Myung Ki Min Beom-Gi Kim Ectopic Expression of OsPYL/RCAR7, an ABA Receptor Having Low Signaling Activity, Improves Drought Tolerance without Growth Defects in Rice International Journal of Molecular Sciences abiotic stress signaling ABA signaling crop growth and stress tolerance balance |
| title | Ectopic Expression of OsPYL/RCAR7, an ABA Receptor Having Low Signaling Activity, Improves Drought Tolerance without Growth Defects in Rice |
| title_full | Ectopic Expression of OsPYL/RCAR7, an ABA Receptor Having Low Signaling Activity, Improves Drought Tolerance without Growth Defects in Rice |
| title_fullStr | Ectopic Expression of OsPYL/RCAR7, an ABA Receptor Having Low Signaling Activity, Improves Drought Tolerance without Growth Defects in Rice |
| title_full_unstemmed | Ectopic Expression of OsPYL/RCAR7, an ABA Receptor Having Low Signaling Activity, Improves Drought Tolerance without Growth Defects in Rice |
| title_short | Ectopic Expression of OsPYL/RCAR7, an ABA Receptor Having Low Signaling Activity, Improves Drought Tolerance without Growth Defects in Rice |
| title_sort | ectopic expression of ospyl rcar7 an aba receptor having low signaling activity improves drought tolerance without growth defects in rice |
| topic | abiotic stress signaling ABA signaling crop growth and stress tolerance balance |
| url | https://www.mdpi.com/1422-0067/21/11/4163 |
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