Data on the first functionally-annotated de novo transcriptome assembly for North American flying squirrels (genus Glaucomys)
We report the first functionally-annotated de novo transcriptome assembly for North American flying squirrels (genus Glaucomys). RNA was extracted from tissue samples obtained from two northern flying squirrels and two southern flying squirrels sampled from Ontario, Canada, and sequenced on an Illum...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-08-01
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| Series: | Data in Brief |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352340921005515 |
| _version_ | 1818572806532628480 |
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| author | Michael G.C. Brown Jeff Bowman Paul J. Wilson |
| author_facet | Michael G.C. Brown Jeff Bowman Paul J. Wilson |
| author_sort | Michael G.C. Brown |
| collection | DOAJ |
| description | We report the first functionally-annotated de novo transcriptome assembly for North American flying squirrels (genus Glaucomys). RNA was extracted from tissue samples obtained from two northern flying squirrels and two southern flying squirrels sampled from Ontario, Canada, and sequenced on an Illumina paired-end sequencing platform. We reconstructed 702,228 Glaucomys transcripts using 193,323,120 sequence read pairs and captured sequence homologies, protein domains, and gene function classifications. Introgressive hybridization between northern (Glaucomys sabrinus) and southern flying squirrels (G. volans) has been observed in some areas of North America. However, existing molecular markers lack the resolution to discriminate late-generation introgressants and describe the extent to which hybridization influences the Glaucomys gene pool. These genomic resources can increase the resolution of molecular techniques used to examine the dynamics of the Glaucomys hybrid zone. |
| first_indexed | 2024-12-15T00:02:34Z |
| format | Article |
| id | doaj.art-806f42853ef24bb18d85b6e3a855cae3 |
| institution | Directory Open Access Journal |
| issn | 2352-3409 |
| language | English |
| last_indexed | 2024-12-15T00:02:34Z |
| publishDate | 2021-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Data in Brief |
| spelling | doaj.art-806f42853ef24bb18d85b6e3a855cae32022-12-21T22:42:51ZengElsevierData in Brief2352-34092021-08-0137107267Data on the first functionally-annotated de novo transcriptome assembly for North American flying squirrels (genus Glaucomys)Michael G.C. Brown0Jeff Bowman1Paul J. Wilson2Environmental and Life Sciences Graduate Program, Trent University, Peterborough, Canada; Corresponding author.Wildlife Research and Monitoring Section, Ontario Ministry of Natural Resources and Forestry, Peterborough, CanadaBiology Department, Trent University, Peterborough, CanadaWe report the first functionally-annotated de novo transcriptome assembly for North American flying squirrels (genus Glaucomys). RNA was extracted from tissue samples obtained from two northern flying squirrels and two southern flying squirrels sampled from Ontario, Canada, and sequenced on an Illumina paired-end sequencing platform. We reconstructed 702,228 Glaucomys transcripts using 193,323,120 sequence read pairs and captured sequence homologies, protein domains, and gene function classifications. Introgressive hybridization between northern (Glaucomys sabrinus) and southern flying squirrels (G. volans) has been observed in some areas of North America. However, existing molecular markers lack the resolution to discriminate late-generation introgressants and describe the extent to which hybridization influences the Glaucomys gene pool. These genomic resources can increase the resolution of molecular techniques used to examine the dynamics of the Glaucomys hybrid zone.http://www.sciencedirect.com/science/article/pii/S2352340921005515RNA-seqTranscriptomeDe novo assemblyFunctional annotationHigh-throughput sequencingHybridization |
| spellingShingle | Michael G.C. Brown Jeff Bowman Paul J. Wilson Data on the first functionally-annotated de novo transcriptome assembly for North American flying squirrels (genus Glaucomys) Data in Brief RNA-seq Transcriptome De novo assembly Functional annotation High-throughput sequencing Hybridization |
| title | Data on the first functionally-annotated de novo transcriptome assembly for North American flying squirrels (genus Glaucomys) |
| title_full | Data on the first functionally-annotated de novo transcriptome assembly for North American flying squirrels (genus Glaucomys) |
| title_fullStr | Data on the first functionally-annotated de novo transcriptome assembly for North American flying squirrels (genus Glaucomys) |
| title_full_unstemmed | Data on the first functionally-annotated de novo transcriptome assembly for North American flying squirrels (genus Glaucomys) |
| title_short | Data on the first functionally-annotated de novo transcriptome assembly for North American flying squirrels (genus Glaucomys) |
| title_sort | data on the first functionally annotated de novo transcriptome assembly for north american flying squirrels genus glaucomys |
| topic | RNA-seq Transcriptome De novo assembly Functional annotation High-throughput sequencing Hybridization |
| url | http://www.sciencedirect.com/science/article/pii/S2352340921005515 |
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