TNF-α − 308 G/A and IFN-γ + 874 A/T gene polymorphisms in Saudi patients with cutaneous leishmaniasis
Abstract Background Cutaneous leishmaniasis (CL) is well linked with immunogenetic factors. This study was undertaken to test the association of TNF-α − 308 and IFN-γ + 874 gene polymorphisms with the susceptibility of Leishmania (L) species among CL patients in central region of Saudi Arabia. Metho...
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| Format: | Article |
| Language: | English |
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BMC
2020-05-01
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| Series: | BMC Medical Genetics |
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| Online Access: | http://link.springer.com/article/10.1186/s12881-020-01043-9 |
| _version_ | 1818560764665921536 |
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| author | Ahmed A. Ahmed Zafar Rasheed Tarek Salem Mohammed S. Al-Dhubaibi Ahmad A. Al Robaee Abdullateef A. Alzolibani |
| author_facet | Ahmed A. Ahmed Zafar Rasheed Tarek Salem Mohammed S. Al-Dhubaibi Ahmad A. Al Robaee Abdullateef A. Alzolibani |
| author_sort | Ahmed A. Ahmed |
| collection | DOAJ |
| description | Abstract Background Cutaneous leishmaniasis (CL) is well linked with immunogenetic factors. This study was undertaken to test the association of TNF-α − 308 and IFN-γ + 874 gene polymorphisms with the susceptibility of Leishmania (L) species among CL patients in central region of Saudi Arabia. Methods This is a case-control study involved 169 Saudi subjects with different L. species and 199 healthy controls from central region of Saudi Arabia. All subjects were characterized by TNF-α − 308 G/A and IFN-γ + 874 A/T gene polymorphisms using PCR. Results Evaluation of genotyping and allelic frequency of TNF-α − 308 G/A in different L. species showed no significant association compared to controls (p > 0.05). Except, in cases of L. tropica that showed significantly higher TNF-α − 308 A versus G allele frequency (p = 0.0004). Evaluation of genotyping of IFN-γ + 874 (TT versus AA+AT recessive) and allelic frequency of IFN-γ + 874 (T versus A) showed significant higher in L. major and also in total CL cases as compared to healthy controls (p < 0.05). Furthermore, a strong association was observed between the susceptibility of L. major, L. tropica or total CL cases with synergistically combined high TNF-α 308/INF-γ 874 alleles. Conclusions This is the first report that shows the gene polymorphisms of TNF-α − 308 G/A and IFN-γ + 874 A/T in Saudi patients with different L. species infections. Data showed that the TNF-α-308 G/A gene polymorphism is not associated with the susceptibility of CL in Saudi subjects. The only correlation was found in between A versus G allelic frequency in L. tropica. Importantly, IFN-γ + 874 A/T polymorphism was found to be associated with the susceptibility of L. major and also with total CL subjects. Moreover, data from synergistically combined high TNF-α 308/INF-γ 874 alleles strongly suggest their potential role in the susceptibility of leishmania infection. |
| first_indexed | 2024-12-14T00:42:27Z |
| format | Article |
| id | doaj.art-8073e45779054215a049cee96f4c4526 |
| institution | Directory Open Access Journal |
| issn | 1471-2350 |
| language | English |
| last_indexed | 2024-12-14T00:42:27Z |
| publishDate | 2020-05-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Medical Genetics |
| spelling | doaj.art-8073e45779054215a049cee96f4c45262022-12-21T23:24:18ZengBMCBMC Medical Genetics1471-23502020-05-012111910.1186/s12881-020-01043-9TNF-α − 308 G/A and IFN-γ + 874 A/T gene polymorphisms in Saudi patients with cutaneous leishmaniasisAhmed A. Ahmed0Zafar Rasheed1Tarek Salem2Mohammed S. Al-Dhubaibi3Ahmad A. Al Robaee4Abdullateef A. Alzolibani5Research Center, College of Medicine, Qassim UniversityDepartment of Medical Biochemistry, College of Medicine, Qassim UniversityDepartment of Medical Biochemistry, College of Medicine, Qassim UniversityDepartment of Dermatology, College of Medicine, Qassim UniversityDepartment of Dermatology, College of Medicine, Qassim UniversityDepartment of Dermatology, College of Medicine, Qassim UniversityAbstract Background Cutaneous leishmaniasis (CL) is well linked with immunogenetic factors. This study was undertaken to test the association of TNF-α − 308 and IFN-γ + 874 gene polymorphisms with the susceptibility of Leishmania (L) species among CL patients in central region of Saudi Arabia. Methods This is a case-control study involved 169 Saudi subjects with different L. species and 199 healthy controls from central region of Saudi Arabia. All subjects were characterized by TNF-α − 308 G/A and IFN-γ + 874 A/T gene polymorphisms using PCR. Results Evaluation of genotyping and allelic frequency of TNF-α − 308 G/A in different L. species showed no significant association compared to controls (p > 0.05). Except, in cases of L. tropica that showed significantly higher TNF-α − 308 A versus G allele frequency (p = 0.0004). Evaluation of genotyping of IFN-γ + 874 (TT versus AA+AT recessive) and allelic frequency of IFN-γ + 874 (T versus A) showed significant higher in L. major and also in total CL cases as compared to healthy controls (p < 0.05). Furthermore, a strong association was observed between the susceptibility of L. major, L. tropica or total CL cases with synergistically combined high TNF-α 308/INF-γ 874 alleles. Conclusions This is the first report that shows the gene polymorphisms of TNF-α − 308 G/A and IFN-γ + 874 A/T in Saudi patients with different L. species infections. Data showed that the TNF-α-308 G/A gene polymorphism is not associated with the susceptibility of CL in Saudi subjects. The only correlation was found in between A versus G allelic frequency in L. tropica. Importantly, IFN-γ + 874 A/T polymorphism was found to be associated with the susceptibility of L. major and also with total CL subjects. Moreover, data from synergistically combined high TNF-α 308/INF-γ 874 alleles strongly suggest their potential role in the susceptibility of leishmania infection.http://link.springer.com/article/10.1186/s12881-020-01043-9Cutaneous leishmaniasisGene polymorphismTNF-αIFN-γL. majorL. tropica |
| spellingShingle | Ahmed A. Ahmed Zafar Rasheed Tarek Salem Mohammed S. Al-Dhubaibi Ahmad A. Al Robaee Abdullateef A. Alzolibani TNF-α − 308 G/A and IFN-γ + 874 A/T gene polymorphisms in Saudi patients with cutaneous leishmaniasis BMC Medical Genetics Cutaneous leishmaniasis Gene polymorphism TNF-α IFN-γ L. major L. tropica |
| title | TNF-α − 308 G/A and IFN-γ + 874 A/T gene polymorphisms in Saudi patients with cutaneous leishmaniasis |
| title_full | TNF-α − 308 G/A and IFN-γ + 874 A/T gene polymorphisms in Saudi patients with cutaneous leishmaniasis |
| title_fullStr | TNF-α − 308 G/A and IFN-γ + 874 A/T gene polymorphisms in Saudi patients with cutaneous leishmaniasis |
| title_full_unstemmed | TNF-α − 308 G/A and IFN-γ + 874 A/T gene polymorphisms in Saudi patients with cutaneous leishmaniasis |
| title_short | TNF-α − 308 G/A and IFN-γ + 874 A/T gene polymorphisms in Saudi patients with cutaneous leishmaniasis |
| title_sort | tnf α 308 g a and ifn γ 874 a t gene polymorphisms in saudi patients with cutaneous leishmaniasis |
| topic | Cutaneous leishmaniasis Gene polymorphism TNF-α IFN-γ L. major L. tropica |
| url | http://link.springer.com/article/10.1186/s12881-020-01043-9 |
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