Estrone degrading enzymes of Spirulina CPCC-695 and synthesis of bioplastic precursor as a by-product
Estrone, a steroidal estrogen that is persistently contaminating the surface water has been classified as an endocrine disruptor and as Group-1 carcinogen by the World Health Organization. Long-term exposure to estrone-contaminated water disrupt physiology, behaviour and sexual development of living...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020-06-01
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| Series: | Biotechnology Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2215017X19306964 |
| _version_ | 1818261979753611264 |
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| author | Neha Sami Sabbir Ansari Durdana Yasin Tasneem Fatma |
| author_facet | Neha Sami Sabbir Ansari Durdana Yasin Tasneem Fatma |
| author_sort | Neha Sami |
| collection | DOAJ |
| description | Estrone, a steroidal estrogen that is persistently contaminating the surface water has been classified as an endocrine disruptor and as Group-1 carcinogen by the World Health Organization. Long-term exposure to estrone-contaminated water disrupt physiology, behaviour and sexual development of living organisms that lead to many disorders. So, it has to be eliminated from our surrounding. Its biological degradation is a cost effective and eco-friendly approach. The present study targets to predict the degradation pathway and understand the role of cyanobacterial enzymes: oxidoreductases (laccase, peroxidase) and esterase in estrone degradation. Poly-β-hydroxy butyrate (PHB) was also quantified as a by-product of estrone biodegradation. The estrone degradation pathway was predicted using EAWAG-BBD/PPS database. Spirulina CPCC-695 was grown in different concentration of estrone (20 mg/l, 50 mg/l, 100 mg/l and 200 mg/l). The culture without estrone was considered as control. The culture supernatant was used for testing laccase and esterase activity whereas the biomass was used to test peroxidase activity and quantify by-product (PHB). The enzymes showed concentration-dependent activities. Maximum enzyme activities were seen at 20 mg/l estrone. Spirulina CPCC-695 utilizes estrone as a carbon source and degrades it to produce pyruvate which forms acetyl CoA that undergo condensation, reduction and polymerization to form PHB. Maximum PHB (169 μg) was also produced at 20 mg/l as a by-product during degradation. |
| first_indexed | 2024-12-12T18:55:50Z |
| format | Article |
| id | doaj.art-80759ee9253c415888a109b7d2401c66 |
| institution | Directory Open Access Journal |
| issn | 2215-017X |
| language | English |
| last_indexed | 2024-12-12T18:55:50Z |
| publishDate | 2020-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biotechnology Reports |
| spelling | doaj.art-80759ee9253c415888a109b7d2401c662022-12-22T00:15:16ZengElsevierBiotechnology Reports2215-017X2020-06-0126e00464Estrone degrading enzymes of Spirulina CPCC-695 and synthesis of bioplastic precursor as a by-productNeha Sami0Sabbir Ansari1Durdana Yasin2Tasneem Fatma3Department of Biosciences, Jamia Millia Islamia, New Delhi 110025, IndiaDepartment of Biosciences, Jamia Millia Islamia, New Delhi 110025, IndiaDepartment of Biosciences, Jamia Millia Islamia, New Delhi 110025, IndiaCorresponding author.; Department of Biosciences, Jamia Millia Islamia, New Delhi 110025, IndiaEstrone, a steroidal estrogen that is persistently contaminating the surface water has been classified as an endocrine disruptor and as Group-1 carcinogen by the World Health Organization. Long-term exposure to estrone-contaminated water disrupt physiology, behaviour and sexual development of living organisms that lead to many disorders. So, it has to be eliminated from our surrounding. Its biological degradation is a cost effective and eco-friendly approach. The present study targets to predict the degradation pathway and understand the role of cyanobacterial enzymes: oxidoreductases (laccase, peroxidase) and esterase in estrone degradation. Poly-β-hydroxy butyrate (PHB) was also quantified as a by-product of estrone biodegradation. The estrone degradation pathway was predicted using EAWAG-BBD/PPS database. Spirulina CPCC-695 was grown in different concentration of estrone (20 mg/l, 50 mg/l, 100 mg/l and 200 mg/l). The culture without estrone was considered as control. The culture supernatant was used for testing laccase and esterase activity whereas the biomass was used to test peroxidase activity and quantify by-product (PHB). The enzymes showed concentration-dependent activities. Maximum enzyme activities were seen at 20 mg/l estrone. Spirulina CPCC-695 utilizes estrone as a carbon source and degrades it to produce pyruvate which forms acetyl CoA that undergo condensation, reduction and polymerization to form PHB. Maximum PHB (169 μg) was also produced at 20 mg/l as a by-product during degradation.http://www.sciencedirect.com/science/article/pii/S2215017X19306964EstroneEndocrine disrupting compoundLaccasePeroxidaseEsterase |
| spellingShingle | Neha Sami Sabbir Ansari Durdana Yasin Tasneem Fatma Estrone degrading enzymes of Spirulina CPCC-695 and synthesis of bioplastic precursor as a by-product Biotechnology Reports Estrone Endocrine disrupting compound Laccase Peroxidase Esterase |
| title | Estrone degrading enzymes of Spirulina CPCC-695 and synthesis of bioplastic precursor as a by-product |
| title_full | Estrone degrading enzymes of Spirulina CPCC-695 and synthesis of bioplastic precursor as a by-product |
| title_fullStr | Estrone degrading enzymes of Spirulina CPCC-695 and synthesis of bioplastic precursor as a by-product |
| title_full_unstemmed | Estrone degrading enzymes of Spirulina CPCC-695 and synthesis of bioplastic precursor as a by-product |
| title_short | Estrone degrading enzymes of Spirulina CPCC-695 and synthesis of bioplastic precursor as a by-product |
| title_sort | estrone degrading enzymes of spirulina cpcc 695 and synthesis of bioplastic precursor as a by product |
| topic | Estrone Endocrine disrupting compound Laccase Peroxidase Esterase |
| url | http://www.sciencedirect.com/science/article/pii/S2215017X19306964 |
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