Isolation and Functional Characterization of Soybean <i>BES1</i>/<i>BZR1</i> <i>Homolog</i> <i>3</i>-<i>Like</i> <i>1</i> (<i>GmBEH3L1</i>) Associated with Dehydration Sensitivity and Brassinosteroid Signaling in <i>Arabidopsis</i> <i>thaliana</i>
Brassinosteroid (BR) is an important steroid hormone that regulates plant development, abscisic acid (ABA) signaling, and responses to abiotic stress. We previously demonstrated that BEH3 (BES1/BZR1 Homolog 3) of <i>Arabidopsis</i> <i>thaliana</i> regulates dehydration and AB...
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MDPI AG
2022-09-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/11/19/2565 |
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| author | Cho-Rong Park Van Tinh Nguyen Ji-Hee Min Hyunkyu Sang Gah-Hyun Lim Cheol Soo Kim |
| author_facet | Cho-Rong Park Van Tinh Nguyen Ji-Hee Min Hyunkyu Sang Gah-Hyun Lim Cheol Soo Kim |
| author_sort | Cho-Rong Park |
| collection | DOAJ |
| description | Brassinosteroid (BR) is an important steroid hormone that regulates plant development, abscisic acid (ABA) signaling, and responses to abiotic stress. We previously demonstrated that BEH3 (BES1/BZR1 Homolog 3) of <i>Arabidopsis</i> <i>thaliana</i> regulates dehydration and ABA responses by mediating proline metabolism. Furthermore, BEH3 negatively regulates BR-mediated hypocotyl elongation in dark-grown seedlings. However, the roles of <i>BEH3</i> ortholog genes in the osmotic stress response of plants have remained largely unknown. Here, <i>GmBEH3L1</i> (<i>Glycine max BEH3-Like 1</i>), a soybean (<i>G.</i> <i>max</i>) ortholog of the <i>BEH3</i> gene of <i>A. thaliana</i>, was isolated and functionally characterized. <i>GmBEH3L1</i> is induced by ABA, dehydration, and drought conditions. The <i>GmBEH3L1</i>-overexpressing transgenic lines (<i>GmBEH3L1</i>-OE/<i>beh3</i>) with the <i>beh3</i> mutant background have ABA- and dehydration-sensitive phenotypes during early seedling growth, implying that GmBEH3L1 is involved in both osmotic stress and ABA sensitivity as a negative regulator in <i>A. thaliana</i>. Consistent with these results, <i>GmBEH3L1</i>-OE/<i>beh3</i> complemental lines exhibit decreased expression levels of ABA- or dehydration-inducible genes. Under darkness, <i>GmBEH3L1</i>-OE/<i>beh3</i> complemental lines display a short hypocotyl length compared to the <i>beh3</i> mutant, indicating that GmBEH3L1 is linked to BR signaling. Together, our data suggest that GmBEH3L1 participates negatively in ABA and dehydration responses through BR signaling. |
| first_indexed | 2024-03-09T21:17:56Z |
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| language | English |
| last_indexed | 2024-03-09T21:17:56Z |
| publishDate | 2022-09-01 |
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| spelling | doaj.art-80763264ea5b4d5798d41c0860c3ee832023-11-23T21:29:35ZengMDPI AGPlants2223-77472022-09-011119256510.3390/plants11192565Isolation and Functional Characterization of Soybean <i>BES1</i>/<i>BZR1</i> <i>Homolog</i> <i>3</i>-<i>Like</i> <i>1</i> (<i>GmBEH3L1</i>) Associated with Dehydration Sensitivity and Brassinosteroid Signaling in <i>Arabidopsis</i> <i>thaliana</i>Cho-Rong Park0Van Tinh Nguyen1Ji-Hee Min2Hyunkyu Sang3Gah-Hyun Lim4Cheol Soo Kim5Department of Applied Biology, Chonnam National University, Gwangju 61186, KoreaDepartment of Applied Biology, Chonnam National University, Gwangju 61186, KoreaDepartment of Biochemistry and Biophysics, Texas A&M University, 300 Olsen Blvd, College Station, TX 77843-2128, USADepartment of Integrative Food, Bioscience and Biotechnology, Chonnam National University, Gwangju 61186, KoreaDepartment of Biological Sciences, Pusan National University, Busan 46241, KoreaDepartment of Applied Biology, Chonnam National University, Gwangju 61186, KoreaBrassinosteroid (BR) is an important steroid hormone that regulates plant development, abscisic acid (ABA) signaling, and responses to abiotic stress. We previously demonstrated that BEH3 (BES1/BZR1 Homolog 3) of <i>Arabidopsis</i> <i>thaliana</i> regulates dehydration and ABA responses by mediating proline metabolism. Furthermore, BEH3 negatively regulates BR-mediated hypocotyl elongation in dark-grown seedlings. However, the roles of <i>BEH3</i> ortholog genes in the osmotic stress response of plants have remained largely unknown. Here, <i>GmBEH3L1</i> (<i>Glycine max BEH3-Like 1</i>), a soybean (<i>G.</i> <i>max</i>) ortholog of the <i>BEH3</i> gene of <i>A. thaliana</i>, was isolated and functionally characterized. <i>GmBEH3L1</i> is induced by ABA, dehydration, and drought conditions. The <i>GmBEH3L1</i>-overexpressing transgenic lines (<i>GmBEH3L1</i>-OE/<i>beh3</i>) with the <i>beh3</i> mutant background have ABA- and dehydration-sensitive phenotypes during early seedling growth, implying that GmBEH3L1 is involved in both osmotic stress and ABA sensitivity as a negative regulator in <i>A. thaliana</i>. Consistent with these results, <i>GmBEH3L1</i>-OE/<i>beh3</i> complemental lines exhibit decreased expression levels of ABA- or dehydration-inducible genes. Under darkness, <i>GmBEH3L1</i>-OE/<i>beh3</i> complemental lines display a short hypocotyl length compared to the <i>beh3</i> mutant, indicating that GmBEH3L1 is linked to BR signaling. Together, our data suggest that GmBEH3L1 participates negatively in ABA and dehydration responses through BR signaling.https://www.mdpi.com/2223-7747/11/19/2565abscisic acidBEH3 orthologbrassinosteroidosmotic stresssoybean GmBEH3L1 |
| spellingShingle | Cho-Rong Park Van Tinh Nguyen Ji-Hee Min Hyunkyu Sang Gah-Hyun Lim Cheol Soo Kim Isolation and Functional Characterization of Soybean <i>BES1</i>/<i>BZR1</i> <i>Homolog</i> <i>3</i>-<i>Like</i> <i>1</i> (<i>GmBEH3L1</i>) Associated with Dehydration Sensitivity and Brassinosteroid Signaling in <i>Arabidopsis</i> <i>thaliana</i> Plants abscisic acid BEH3 ortholog brassinosteroid osmotic stress soybean GmBEH3L1 |
| title | Isolation and Functional Characterization of Soybean <i>BES1</i>/<i>BZR1</i> <i>Homolog</i> <i>3</i>-<i>Like</i> <i>1</i> (<i>GmBEH3L1</i>) Associated with Dehydration Sensitivity and Brassinosteroid Signaling in <i>Arabidopsis</i> <i>thaliana</i> |
| title_full | Isolation and Functional Characterization of Soybean <i>BES1</i>/<i>BZR1</i> <i>Homolog</i> <i>3</i>-<i>Like</i> <i>1</i> (<i>GmBEH3L1</i>) Associated with Dehydration Sensitivity and Brassinosteroid Signaling in <i>Arabidopsis</i> <i>thaliana</i> |
| title_fullStr | Isolation and Functional Characterization of Soybean <i>BES1</i>/<i>BZR1</i> <i>Homolog</i> <i>3</i>-<i>Like</i> <i>1</i> (<i>GmBEH3L1</i>) Associated with Dehydration Sensitivity and Brassinosteroid Signaling in <i>Arabidopsis</i> <i>thaliana</i> |
| title_full_unstemmed | Isolation and Functional Characterization of Soybean <i>BES1</i>/<i>BZR1</i> <i>Homolog</i> <i>3</i>-<i>Like</i> <i>1</i> (<i>GmBEH3L1</i>) Associated with Dehydration Sensitivity and Brassinosteroid Signaling in <i>Arabidopsis</i> <i>thaliana</i> |
| title_short | Isolation and Functional Characterization of Soybean <i>BES1</i>/<i>BZR1</i> <i>Homolog</i> <i>3</i>-<i>Like</i> <i>1</i> (<i>GmBEH3L1</i>) Associated with Dehydration Sensitivity and Brassinosteroid Signaling in <i>Arabidopsis</i> <i>thaliana</i> |
| title_sort | isolation and functional characterization of soybean i bes1 i i bzr1 i i homolog i i 3 i i like i i 1 i i gmbeh3l1 i associated with dehydration sensitivity and brassinosteroid signaling in i arabidopsis i i thaliana i |
| topic | abscisic acid BEH3 ortholog brassinosteroid osmotic stress soybean GmBEH3L1 |
| url | https://www.mdpi.com/2223-7747/11/19/2565 |
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