Continuous succinic acid fermentation by Actinobacillus succinogenes in a packed-bed biofilm reactor
Abstract Background Succinic acid is one of the most interesting platform chemicals that can be produced in a biorefinery approach. In this study, continuous succinic acid production by Actinobacillus succinogenes fermentation in a packed-bed biofilm reactor (PBBR) was investigated. Results The effe...
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| Format: | Article |
| Language: | English |
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BMC
2018-05-01
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| Series: | Biotechnology for Biofuels |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13068-018-1143-7 |
| _version_ | 1817978406575276032 |
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| author | Mariateresa Ferone Francesca Raganati Alessia Ercole Giuseppe Olivieri Piero Salatino Antonio Marzocchella |
| author_facet | Mariateresa Ferone Francesca Raganati Alessia Ercole Giuseppe Olivieri Piero Salatino Antonio Marzocchella |
| author_sort | Mariateresa Ferone |
| collection | DOAJ |
| description | Abstract Background Succinic acid is one of the most interesting platform chemicals that can be produced in a biorefinery approach. In this study, continuous succinic acid production by Actinobacillus succinogenes fermentation in a packed-bed biofilm reactor (PBBR) was investigated. Results The effects of the operating conditions tested, dilution rate (D), and medium composition (mixture of glucose, xylose, and arabinose—that simulate the composition of a lignocellulosic hydrolysate)—on the PBBR performances were investigated. The maximum succinic acid productivity of 35.0 g L−1 h−1 and the maximum SA concentration were achieved at a D = 1.9 h−1. The effect of HMF and furfural on succinic acid production was also investigated. HMF resulted to reduce succinic acid production by 22.6%, while furfural caused a reduction of 16% in SA production at the same dilution rate. Conclusion Succinic acid production by A. succinogenes fermentation in a packed-bed reactor (PBBR) was successfully carried out for more than 5 months. The optimal results were obtained at the dilution rate 0.5 h−1: 43.0 g L−1 of succinic acid were produced, glucose conversion was 88%; and the volumetric productivity was 22 g L−1 h−1. |
| first_indexed | 2024-04-13T22:29:37Z |
| format | Article |
| id | doaj.art-80783e17c2fc4d1c93286f1571c847be |
| institution | Directory Open Access Journal |
| issn | 1754-6834 |
| language | English |
| last_indexed | 2024-04-13T22:29:37Z |
| publishDate | 2018-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Biotechnology for Biofuels |
| spelling | doaj.art-80783e17c2fc4d1c93286f1571c847be2022-12-22T02:26:58ZengBMCBiotechnology for Biofuels1754-68342018-05-0111111110.1186/s13068-018-1143-7Continuous succinic acid fermentation by Actinobacillus succinogenes in a packed-bed biofilm reactorMariateresa Ferone0Francesca Raganati1Alessia Ercole2Giuseppe Olivieri3Piero Salatino4Antonio Marzocchella5Dipartimento di Ingegneria Chimica, dei Materiali e della Produzione Industriale, Università degli Studi di Napoli Federico IIDipartimento di Ingegneria Chimica, dei Materiali e della Produzione Industriale, Università degli Studi di Napoli Federico IIDipartimento di Ingegneria Chimica, dei Materiali e della Produzione Industriale, Università degli Studi di Napoli Federico IIDipartimento di Ingegneria Chimica, dei Materiali e della Produzione Industriale, Università degli Studi di Napoli Federico IIDipartimento di Ingegneria Chimica, dei Materiali e della Produzione Industriale, Università degli Studi di Napoli Federico IIDipartimento di Ingegneria Chimica, dei Materiali e della Produzione Industriale, Università degli Studi di Napoli Federico IIAbstract Background Succinic acid is one of the most interesting platform chemicals that can be produced in a biorefinery approach. In this study, continuous succinic acid production by Actinobacillus succinogenes fermentation in a packed-bed biofilm reactor (PBBR) was investigated. Results The effects of the operating conditions tested, dilution rate (D), and medium composition (mixture of glucose, xylose, and arabinose—that simulate the composition of a lignocellulosic hydrolysate)—on the PBBR performances were investigated. The maximum succinic acid productivity of 35.0 g L−1 h−1 and the maximum SA concentration were achieved at a D = 1.9 h−1. The effect of HMF and furfural on succinic acid production was also investigated. HMF resulted to reduce succinic acid production by 22.6%, while furfural caused a reduction of 16% in SA production at the same dilution rate. Conclusion Succinic acid production by A. succinogenes fermentation in a packed-bed reactor (PBBR) was successfully carried out for more than 5 months. The optimal results were obtained at the dilution rate 0.5 h−1: 43.0 g L−1 of succinic acid were produced, glucose conversion was 88%; and the volumetric productivity was 22 g L−1 h−1.http://link.springer.com/article/10.1186/s13068-018-1143-7BiorefinerySuccinic acidActinobacillus succinogenesBiofilmLignocellulose |
| spellingShingle | Mariateresa Ferone Francesca Raganati Alessia Ercole Giuseppe Olivieri Piero Salatino Antonio Marzocchella Continuous succinic acid fermentation by Actinobacillus succinogenes in a packed-bed biofilm reactor Biotechnology for Biofuels Biorefinery Succinic acid Actinobacillus succinogenes Biofilm Lignocellulose |
| title | Continuous succinic acid fermentation by Actinobacillus succinogenes in a packed-bed biofilm reactor |
| title_full | Continuous succinic acid fermentation by Actinobacillus succinogenes in a packed-bed biofilm reactor |
| title_fullStr | Continuous succinic acid fermentation by Actinobacillus succinogenes in a packed-bed biofilm reactor |
| title_full_unstemmed | Continuous succinic acid fermentation by Actinobacillus succinogenes in a packed-bed biofilm reactor |
| title_short | Continuous succinic acid fermentation by Actinobacillus succinogenes in a packed-bed biofilm reactor |
| title_sort | continuous succinic acid fermentation by actinobacillus succinogenes in a packed bed biofilm reactor |
| topic | Biorefinery Succinic acid Actinobacillus succinogenes Biofilm Lignocellulose |
| url | http://link.springer.com/article/10.1186/s13068-018-1143-7 |
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