Application of Acetate as a Substrate for the Production of Value-Added Chemicals in <em>Escherichia coli</em>
At present, the production of the majority of valuable chemicals is dependent on the microbial fermentation of carbohydrate substrates. However, direct competition is a potential problem for microbial feedstocks that are also used within the food/feed industries. The use of alternative carbon source...
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MDPI AG
2024-02-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/12/2/309 |
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| author | Pengfei Gu Fangfang Li Zhaosong Huang Juan Gao |
| author_facet | Pengfei Gu Fangfang Li Zhaosong Huang Juan Gao |
| author_sort | Pengfei Gu |
| collection | DOAJ |
| description | At present, the production of the majority of valuable chemicals is dependent on the microbial fermentation of carbohydrate substrates. However, direct competition is a potential problem for microbial feedstocks that are also used within the food/feed industries. The use of alternative carbon sources, such as acetate, has therefore become a research focus. As a common organic acid, acetate can be generated from lignocellulosic biomass and C1 gases, as well as being a major byproduct in microbial fermentation, especially in the presence of an excess carbon source. As a model microorganism, <i>Escherichia coli</i> has been widely applied in the production of valuable chemicals using different carbon sources. Recently, several valuable chemicals (e.g., succinic acid, itaconic acid, isobutanol, and mevalonic acid) have been investigated for synthesis in <i>E. coli</i> using acetate as the sole carbon source. In this review, we summarize the acetate metabolic pathway in <i>E. coli</i> and recent research into the microbial production of chemical compounds in <i>E. coli</i> using acetate as the carbon source. Although microbial synthetic pathways for different compounds have been developed in <i>E. coli</i>, the production titer and yield are insufficient for commercial applications. Finally, we discuss the development prospects and challenges of using acetate for microbial fermentation. |
| first_indexed | 2024-03-07T22:20:59Z |
| format | Article |
| id | doaj.art-80784c131d0149f78c15b03b0387b5c6 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-07T22:20:59Z |
| publishDate | 2024-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-80784c131d0149f78c15b03b0387b5c62024-02-23T15:28:09ZengMDPI AGMicroorganisms2076-26072024-02-0112230910.3390/microorganisms12020309Application of Acetate as a Substrate for the Production of Value-Added Chemicals in <em>Escherichia coli</em>Pengfei Gu0Fangfang Li1Zhaosong Huang2Juan Gao3School of Biological Science and Technology, University of Jinan, Jinan 250022, ChinaYantai Food and Drug Control and Test Center, Yantai 264003, ChinaSchool of Biological Science and Technology, University of Jinan, Jinan 250022, ChinaSchool of Biological Science and Technology, University of Jinan, Jinan 250022, ChinaAt present, the production of the majority of valuable chemicals is dependent on the microbial fermentation of carbohydrate substrates. However, direct competition is a potential problem for microbial feedstocks that are also used within the food/feed industries. The use of alternative carbon sources, such as acetate, has therefore become a research focus. As a common organic acid, acetate can be generated from lignocellulosic biomass and C1 gases, as well as being a major byproduct in microbial fermentation, especially in the presence of an excess carbon source. As a model microorganism, <i>Escherichia coli</i> has been widely applied in the production of valuable chemicals using different carbon sources. Recently, several valuable chemicals (e.g., succinic acid, itaconic acid, isobutanol, and mevalonic acid) have been investigated for synthesis in <i>E. coli</i> using acetate as the sole carbon source. In this review, we summarize the acetate metabolic pathway in <i>E. coli</i> and recent research into the microbial production of chemical compounds in <i>E. coli</i> using acetate as the carbon source. Although microbial synthetic pathways for different compounds have been developed in <i>E. coli</i>, the production titer and yield are insufficient for commercial applications. Finally, we discuss the development prospects and challenges of using acetate for microbial fermentation.https://www.mdpi.com/2076-2607/12/2/309acetatevaluable chemicals<i>E. coli</i>metabolismtolerance |
| spellingShingle | Pengfei Gu Fangfang Li Zhaosong Huang Juan Gao Application of Acetate as a Substrate for the Production of Value-Added Chemicals in <em>Escherichia coli</em> Microorganisms acetate valuable chemicals <i>E. coli</i> metabolism tolerance |
| title | Application of Acetate as a Substrate for the Production of Value-Added Chemicals in <em>Escherichia coli</em> |
| title_full | Application of Acetate as a Substrate for the Production of Value-Added Chemicals in <em>Escherichia coli</em> |
| title_fullStr | Application of Acetate as a Substrate for the Production of Value-Added Chemicals in <em>Escherichia coli</em> |
| title_full_unstemmed | Application of Acetate as a Substrate for the Production of Value-Added Chemicals in <em>Escherichia coli</em> |
| title_short | Application of Acetate as a Substrate for the Production of Value-Added Chemicals in <em>Escherichia coli</em> |
| title_sort | application of acetate as a substrate for the production of value added chemicals in em escherichia coli em |
| topic | acetate valuable chemicals <i>E. coli</i> metabolism tolerance |
| url | https://www.mdpi.com/2076-2607/12/2/309 |
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