Growth and Biochemical Composition of <i>Porphyridium</i> <i>purpureum</i> SCS-02 under Different Nitrogen Concentrations
Microalgae of the genus <i>Porphyridium</i> show great potential for large-scale commercial cultivation, as they accumulate large quantities of B-phycoerythrin (B-PE), long chain polyunsaturated fatty acids (LC-PUFAs) and exopolysaccharide (EPS). The present study aimed to adjust culture...
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| Format: | Article |
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MDPI AG
2019-02-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/17/2/124 |
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| author | Tao Li Jin Xu Houbo Wu Peiliang Jiang Zishuo Chen Wenzhou Xiang |
| author_facet | Tao Li Jin Xu Houbo Wu Peiliang Jiang Zishuo Chen Wenzhou Xiang |
| author_sort | Tao Li |
| collection | DOAJ |
| description | Microalgae of the genus <i>Porphyridium</i> show great potential for large-scale commercial cultivation, as they accumulate large quantities of B-phycoerythrin (B-PE), long chain polyunsaturated fatty acids (LC-PUFAs) and exopolysaccharide (EPS). The present study aimed to adjust culture nitrogen concentrations to produce <i>Porphyridium</i> biomass rich in B-PE, LC-PUFAs and EPS. <i>Porphyridium purpureum</i> SCS-02 was cultured in ASW culture medium with low nitrogen supply (LN, 3.5 mM), medium nitrogen supply (MN, 5.9 mM) or high nitrogen supply (HN, 17.6 mM). HN significantly enhanced the accumulation of biomass, intracellular protein, B-PE and eicosapentaenoic acid. LN increased the intracellular carbohydrate and arachidonic acid content, and promoted the secretion of EPS. The total lipids content was almost unaffected by nitrogen concentration. Based on these results, a semi-continuous two-step process was proposed, which included the production of biomass rich in B-PE and LC-PUFAs with sufficient nitrogen, and induced EPS excretion with limited nitrogen and strong light. |
| first_indexed | 2024-04-11T20:54:48Z |
| format | Article |
| id | doaj.art-807bd79650954cecb93948e51b7d52b4 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-04-11T20:54:48Z |
| publishDate | 2019-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-807bd79650954cecb93948e51b7d52b42022-12-22T04:03:43ZengMDPI AGMarine Drugs1660-33972019-02-0117212410.3390/md17020124md17020124Growth and Biochemical Composition of <i>Porphyridium</i> <i>purpureum</i> SCS-02 under Different Nitrogen ConcentrationsTao Li0Jin Xu1Houbo Wu2Peiliang Jiang3Zishuo Chen4Wenzhou Xiang5CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, Institution of South China Sea Ecology and Environmental Engineering, RNAM Center for Marine Microbiology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, ChinaKey Laboratory of Renewable Energy, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, ChinaCAS Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, Institution of South China Sea Ecology and Environmental Engineering, RNAM Center for Marine Microbiology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, ChinaKey Laboratory of Renewable Energy, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, ChinaCAS Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, Institution of South China Sea Ecology and Environmental Engineering, RNAM Center for Marine Microbiology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, ChinaCAS Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, Institution of South China Sea Ecology and Environmental Engineering, RNAM Center for Marine Microbiology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, ChinaMicroalgae of the genus <i>Porphyridium</i> show great potential for large-scale commercial cultivation, as they accumulate large quantities of B-phycoerythrin (B-PE), long chain polyunsaturated fatty acids (LC-PUFAs) and exopolysaccharide (EPS). The present study aimed to adjust culture nitrogen concentrations to produce <i>Porphyridium</i> biomass rich in B-PE, LC-PUFAs and EPS. <i>Porphyridium purpureum</i> SCS-02 was cultured in ASW culture medium with low nitrogen supply (LN, 3.5 mM), medium nitrogen supply (MN, 5.9 mM) or high nitrogen supply (HN, 17.6 mM). HN significantly enhanced the accumulation of biomass, intracellular protein, B-PE and eicosapentaenoic acid. LN increased the intracellular carbohydrate and arachidonic acid content, and promoted the secretion of EPS. The total lipids content was almost unaffected by nitrogen concentration. Based on these results, a semi-continuous two-step process was proposed, which included the production of biomass rich in B-PE and LC-PUFAs with sufficient nitrogen, and induced EPS excretion with limited nitrogen and strong light.https://www.mdpi.com/1660-3397/17/2/124<i>Porphyridium</i>nitrogenphycoerythrinexopolysaccharidepolyunsaturated fatty acids |
| spellingShingle | Tao Li Jin Xu Houbo Wu Peiliang Jiang Zishuo Chen Wenzhou Xiang Growth and Biochemical Composition of <i>Porphyridium</i> <i>purpureum</i> SCS-02 under Different Nitrogen Concentrations Marine Drugs <i>Porphyridium</i> nitrogen phycoerythrin exopolysaccharide polyunsaturated fatty acids |
| title | Growth and Biochemical Composition of <i>Porphyridium</i> <i>purpureum</i> SCS-02 under Different Nitrogen Concentrations |
| title_full | Growth and Biochemical Composition of <i>Porphyridium</i> <i>purpureum</i> SCS-02 under Different Nitrogen Concentrations |
| title_fullStr | Growth and Biochemical Composition of <i>Porphyridium</i> <i>purpureum</i> SCS-02 under Different Nitrogen Concentrations |
| title_full_unstemmed | Growth and Biochemical Composition of <i>Porphyridium</i> <i>purpureum</i> SCS-02 under Different Nitrogen Concentrations |
| title_short | Growth and Biochemical Composition of <i>Porphyridium</i> <i>purpureum</i> SCS-02 under Different Nitrogen Concentrations |
| title_sort | growth and biochemical composition of i porphyridium i i purpureum i scs 02 under different nitrogen concentrations |
| topic | <i>Porphyridium</i> nitrogen phycoerythrin exopolysaccharide polyunsaturated fatty acids |
| url | https://www.mdpi.com/1660-3397/17/2/124 |
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