Genomic and Phenotypic Insights into the Potential of <i>Bacillus subtilis</i> YB-15 Isolated from Rhizosphere to Biocontrol against Crown Rot and Promote Growth of Wheat
<i>Fusarium</i> crown rot caused by <i>Fusarium pseudograminearum</i> is one of the most devastating diseases of wheat worldwide causing major yield and economic losses. In this study, strain YB-15 was isolated from soil of wheat rhizosphere and classified as <i>Bacillu...
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2022-05-01
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| author | Wen Xu Qian Yang Xia Xie Paul H. Goodwin Xiaoxu Deng Jie Zhang Runhong Sun Qi Wang Mingcong Xia Chao Wu Lirong Yang |
| author_facet | Wen Xu Qian Yang Xia Xie Paul H. Goodwin Xiaoxu Deng Jie Zhang Runhong Sun Qi Wang Mingcong Xia Chao Wu Lirong Yang |
| author_sort | Wen Xu |
| collection | DOAJ |
| description | <i>Fusarium</i> crown rot caused by <i>Fusarium pseudograminearum</i> is one of the most devastating diseases of wheat worldwide causing major yield and economic losses. In this study, strain YB-15 was isolated from soil of wheat rhizosphere and classified as <i>Bacillus subtilis</i> by average nucleotide identity analysis. It significantly reduced <i>Fusarium</i> crown rot with a control efficacy of 81.50% and significantly improved the growth of wheat seedlings by increasing root and shoot fresh weight by 11.4% and 4.2%, respectively. Reduced <i>Fusarium</i> crown rot may have been due to direct antagonism by the production of β-1, 3-glucanase, amylase, protease and cellulase, or by the ability of <i>B. subtilis</i> YB-15 to induce defense-related enzyme activities of wheat seedlings, both alone and in seedlings infected with <i>F. pseudograminearum</i>. Improved plant growth may be related to the ability of <i>B. subtilis</i> YB-15 to secrete indole acetic acid and siderophores, as well as to solubilize phosphorus. In addition, the genome of strain YB-15 was determined, resulting in a complete assembled circular genome of 4,233,040 bp with GC content of 43.52% consisting of 4207 protein-encoding genes. Sequencing the <i>B. subtilis</i> YB-15 genome further revealed genes for encoding carbohydrate-active enzymes, biosynthesis of various secondary metabolites, nutrient acquisition, phytohormone production, chemotaxis and motility, which could explain the potential of strain YB-15 to be plant growth-promoting bacteria and biological control agent. <i>B. subtilis</i> YB-15 appears to be a promising biocontrol agent against <i>Fusarium</i> crown rot as well as for wheat growth promotion. |
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| spelling | doaj.art-807ec8d48387438c9de4eea65a02c8e02023-11-23T10:08:19ZengMDPI AGBiology2079-77372022-05-0111577810.3390/biology11050778Genomic and Phenotypic Insights into the Potential of <i>Bacillus subtilis</i> YB-15 Isolated from Rhizosphere to Biocontrol against Crown Rot and Promote Growth of WheatWen Xu0Qian Yang1Xia Xie2Paul H. Goodwin3Xiaoxu Deng4Jie Zhang5Runhong Sun6Qi Wang7Mingcong Xia8Chao Wu9Lirong Yang10Henan International Joint Laboratory of Crop Protection, Henan Biopesticide Engineering Research Center, Institute of Plant Protection Research, Henan Academy of Agricultural Sciences, Zhengzhou 450002, ChinaHenan International Joint Laboratory of Crop Protection, Henan Biopesticide Engineering Research Center, Institute of Plant Protection Research, Henan Academy of Agricultural Sciences, Zhengzhou 450002, ChinaHenan International Joint Laboratory of Crop Protection, Henan Biopesticide Engineering Research Center, Institute of Plant Protection Research, Henan Academy of Agricultural Sciences, Zhengzhou 450002, ChinaSchool of Environmental Sciences, University of Guelph, 50 Stone Road East, Guelph, ON N1G2W1, CanadaHenan International Joint Laboratory of Crop Protection, Henan Biopesticide Engineering Research Center, Institute of Plant Protection Research, Henan Academy of Agricultural Sciences, Zhengzhou 450002, ChinaHenan International Joint Laboratory of Crop Protection, Henan Biopesticide Engineering Research Center, Institute of Plant Protection Research, Henan Academy of Agricultural Sciences, Zhengzhou 450002, ChinaHenan International Joint Laboratory of Crop Protection, Henan Biopesticide Engineering Research Center, Institute of Plant Protection Research, Henan Academy of Agricultural Sciences, Zhengzhou 450002, ChinaCollege of Plant Protection, China Agricultural University, Beijing 100083, ChinaHenan International Joint Laboratory of Crop Protection, Henan Biopesticide Engineering Research Center, Institute of Plant Protection Research, Henan Academy of Agricultural Sciences, Zhengzhou 450002, ChinaHenan International Joint Laboratory of Crop Protection, Henan Biopesticide Engineering Research Center, Institute of Plant Protection Research, Henan Academy of Agricultural Sciences, Zhengzhou 450002, ChinaHenan International Joint Laboratory of Crop Protection, Henan Biopesticide Engineering Research Center, Institute of Plant Protection Research, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China<i>Fusarium</i> crown rot caused by <i>Fusarium pseudograminearum</i> is one of the most devastating diseases of wheat worldwide causing major yield and economic losses. In this study, strain YB-15 was isolated from soil of wheat rhizosphere and classified as <i>Bacillus subtilis</i> by average nucleotide identity analysis. It significantly reduced <i>Fusarium</i> crown rot with a control efficacy of 81.50% and significantly improved the growth of wheat seedlings by increasing root and shoot fresh weight by 11.4% and 4.2%, respectively. Reduced <i>Fusarium</i> crown rot may have been due to direct antagonism by the production of β-1, 3-glucanase, amylase, protease and cellulase, or by the ability of <i>B. subtilis</i> YB-15 to induce defense-related enzyme activities of wheat seedlings, both alone and in seedlings infected with <i>F. pseudograminearum</i>. Improved plant growth may be related to the ability of <i>B. subtilis</i> YB-15 to secrete indole acetic acid and siderophores, as well as to solubilize phosphorus. In addition, the genome of strain YB-15 was determined, resulting in a complete assembled circular genome of 4,233,040 bp with GC content of 43.52% consisting of 4207 protein-encoding genes. Sequencing the <i>B. subtilis</i> YB-15 genome further revealed genes for encoding carbohydrate-active enzymes, biosynthesis of various secondary metabolites, nutrient acquisition, phytohormone production, chemotaxis and motility, which could explain the potential of strain YB-15 to be plant growth-promoting bacteria and biological control agent. <i>B. subtilis</i> YB-15 appears to be a promising biocontrol agent against <i>Fusarium</i> crown rot as well as for wheat growth promotion.https://www.mdpi.com/2079-7737/11/5/778<i>Bacillus subtilis</i><i>Fusarium pseudograminearum</i>wheatbiocontrol agentgenome characterizationgrowth promotion |
| spellingShingle | Wen Xu Qian Yang Xia Xie Paul H. Goodwin Xiaoxu Deng Jie Zhang Runhong Sun Qi Wang Mingcong Xia Chao Wu Lirong Yang Genomic and Phenotypic Insights into the Potential of <i>Bacillus subtilis</i> YB-15 Isolated from Rhizosphere to Biocontrol against Crown Rot and Promote Growth of Wheat Biology <i>Bacillus subtilis</i> <i>Fusarium pseudograminearum</i> wheat biocontrol agent genome characterization growth promotion |
| title | Genomic and Phenotypic Insights into the Potential of <i>Bacillus subtilis</i> YB-15 Isolated from Rhizosphere to Biocontrol against Crown Rot and Promote Growth of Wheat |
| title_full | Genomic and Phenotypic Insights into the Potential of <i>Bacillus subtilis</i> YB-15 Isolated from Rhizosphere to Biocontrol against Crown Rot and Promote Growth of Wheat |
| title_fullStr | Genomic and Phenotypic Insights into the Potential of <i>Bacillus subtilis</i> YB-15 Isolated from Rhizosphere to Biocontrol against Crown Rot and Promote Growth of Wheat |
| title_full_unstemmed | Genomic and Phenotypic Insights into the Potential of <i>Bacillus subtilis</i> YB-15 Isolated from Rhizosphere to Biocontrol against Crown Rot and Promote Growth of Wheat |
| title_short | Genomic and Phenotypic Insights into the Potential of <i>Bacillus subtilis</i> YB-15 Isolated from Rhizosphere to Biocontrol against Crown Rot and Promote Growth of Wheat |
| title_sort | genomic and phenotypic insights into the potential of i bacillus subtilis i yb 15 isolated from rhizosphere to biocontrol against crown rot and promote growth of wheat |
| topic | <i>Bacillus subtilis</i> <i>Fusarium pseudograminearum</i> wheat biocontrol agent genome characterization growth promotion |
| url | https://www.mdpi.com/2079-7737/11/5/778 |
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