Pattern of Protein Expression in Developing Wheat Grains Identified through Proteomic Analysis
Grain development is one of the biological processes, which contributes to the final grain yield. To understand the molecular changes taking place during the early grain development, we profiled proteomes of two common wheat cultivars P271 and Chinese Spring (CS) with large and small grains, respect...
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| Format: | Article |
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Frontiers Media S.A.
2017-06-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fpls.2017.00962/full |
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| author | Mingming Yang Mingming Yang Xiang Gao Xiang Gao Jian Dong Jian Dong Nitant Gandhi Huanjie Cai Huanjie Cai Diter H. von Wettstein Sachin Rustgi Sachin Rustgi Shanshan Wen |
| author_facet | Mingming Yang Mingming Yang Xiang Gao Xiang Gao Jian Dong Jian Dong Nitant Gandhi Huanjie Cai Huanjie Cai Diter H. von Wettstein Sachin Rustgi Sachin Rustgi Shanshan Wen |
| author_sort | Mingming Yang |
| collection | DOAJ |
| description | Grain development is one of the biological processes, which contributes to the final grain yield. To understand the molecular changes taking place during the early grain development, we profiled proteomes of two common wheat cultivars P271 and Chinese Spring (CS) with large and small grains, respectively at three grain developmental stages (4, 8, and 12 days post anthesis). An iTRAQ (isobaric tags for relative and absolute quantitation) based proteomics approach was used for this purpose. More than 3,600 proteins were reported to accumulate during early grain development in both wheat cultivars. Of these 3,600 proteins, 130 expressed differentially between two wheat cultivars, and 306 exhibited developmental stage-specific accumulation in either or both genotypes. Detailed bioinformatic analyses of differentially expressed proteins (DEPs) from the large- and small-grain wheat cultivars underscored the developmental differences observed between them and shed light on the molecular and cellular processes contributing to these differences. In silico localization of either or both sets of DEPs to wheat chromosomes exhibited a biased genomic distribution with chromosome 4D contributing largely to it. These results corresponded well with the earlier studies, performed in common wheat, where chromosome 4D was reported to harbor QTLs for yield contributing traits specifically grain length. Collectively, our results provide insight into the molecular processes taking place during early grain development, a knowledge, which may prove useful in improving wheat grain yield in the future. |
| first_indexed | 2024-12-19T06:06:00Z |
| format | Article |
| id | doaj.art-807ee7b997ee4f73a548748bae10b162 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-19T06:06:00Z |
| publishDate | 2017-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-807ee7b997ee4f73a548748bae10b1622022-12-21T20:33:09ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2017-06-01810.3389/fpls.2017.00962252984Pattern of Protein Expression in Developing Wheat Grains Identified through Proteomic AnalysisMingming Yang0Mingming Yang1Xiang Gao2Xiang Gao3Jian Dong4Jian Dong5Nitant Gandhi6Huanjie Cai7Huanjie Cai8Diter H. von Wettstein9Sachin Rustgi10Sachin Rustgi11Shanshan Wen12State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A&F UniversityYangling, ChinaWheat Engineering Research Center of Shaanxi Province, Northwest A&F UniversityYangling, ChinaState Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A&F UniversityYangling, ChinaWheat Engineering Research Center of Shaanxi Province, Northwest A&F UniversityYangling, ChinaState Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A&F UniversityYangling, ChinaWheat Engineering Research Center of Shaanxi Province, Northwest A&F UniversityYangling, ChinaDepartment of Plant and Environmental Sciences, Clemson University Pee Dee Research and Education CenterFlorence, SC, United StatesKey Laboratory of Agricultural Soil and Water Engineering in Arid and Semiarid Areas, Ministry of Education, Northwest A&F UniversityYangling, ChinaInstitute of Water Saving Agriculture in Arid Regions of China, Northwest A&F UniversityYangling, ChinaDepartment of Crop and Soil Sciences, Washington State UniversityPullman, WA, United StatesDepartment of Plant and Environmental Sciences, Clemson University Pee Dee Research and Education CenterFlorence, SC, United StatesDepartment of Crop and Soil Sciences, Washington State UniversityPullman, WA, United StatesState Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A&F UniversityYangling, ChinaGrain development is one of the biological processes, which contributes to the final grain yield. To understand the molecular changes taking place during the early grain development, we profiled proteomes of two common wheat cultivars P271 and Chinese Spring (CS) with large and small grains, respectively at three grain developmental stages (4, 8, and 12 days post anthesis). An iTRAQ (isobaric tags for relative and absolute quantitation) based proteomics approach was used for this purpose. More than 3,600 proteins were reported to accumulate during early grain development in both wheat cultivars. Of these 3,600 proteins, 130 expressed differentially between two wheat cultivars, and 306 exhibited developmental stage-specific accumulation in either or both genotypes. Detailed bioinformatic analyses of differentially expressed proteins (DEPs) from the large- and small-grain wheat cultivars underscored the developmental differences observed between them and shed light on the molecular and cellular processes contributing to these differences. In silico localization of either or both sets of DEPs to wheat chromosomes exhibited a biased genomic distribution with chromosome 4D contributing largely to it. These results corresponded well with the earlier studies, performed in common wheat, where chromosome 4D was reported to harbor QTLs for yield contributing traits specifically grain length. Collectively, our results provide insight into the molecular processes taking place during early grain development, a knowledge, which may prove useful in improving wheat grain yield in the future.http://journal.frontiersin.org/article/10.3389/fpls.2017.00962/fullwheatgrain developmentmetabolic pathwayproteomechromosome |
| spellingShingle | Mingming Yang Mingming Yang Xiang Gao Xiang Gao Jian Dong Jian Dong Nitant Gandhi Huanjie Cai Huanjie Cai Diter H. von Wettstein Sachin Rustgi Sachin Rustgi Shanshan Wen Pattern of Protein Expression in Developing Wheat Grains Identified through Proteomic Analysis Frontiers in Plant Science wheat grain development metabolic pathway proteome chromosome |
| title | Pattern of Protein Expression in Developing Wheat Grains Identified through Proteomic Analysis |
| title_full | Pattern of Protein Expression in Developing Wheat Grains Identified through Proteomic Analysis |
| title_fullStr | Pattern of Protein Expression in Developing Wheat Grains Identified through Proteomic Analysis |
| title_full_unstemmed | Pattern of Protein Expression in Developing Wheat Grains Identified through Proteomic Analysis |
| title_short | Pattern of Protein Expression in Developing Wheat Grains Identified through Proteomic Analysis |
| title_sort | pattern of protein expression in developing wheat grains identified through proteomic analysis |
| topic | wheat grain development metabolic pathway proteome chromosome |
| url | http://journal.frontiersin.org/article/10.3389/fpls.2017.00962/full |
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