Assessment of developed paper strip based sensor with pesticide residues in different dairy environmental samples

According to the results of this study, the paper strip biosensor can detect pesticide at very low concentration like fungicide, organochlorine, organophosphate, carbamate, and herbicide group ranges from 1 to 10, 1–50, 250–500, 1–50, and 1 μg/L, respectively in animal feed, water, milk and soil. Th...

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Main Authors: Soniya A. Ranveer, C.G. Harshitha, Vaishali Dasriya, Nimisha Tehri, Naresh Kumar, H.V. Raghu
Format: Article
Language:English
Published: Elsevier 2023-01-01
Series:Current Research in Food Science
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2665927122002428
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author Soniya A. Ranveer
C.G. Harshitha
Vaishali Dasriya
Nimisha Tehri
Naresh Kumar
H.V. Raghu
author_facet Soniya A. Ranveer
C.G. Harshitha
Vaishali Dasriya
Nimisha Tehri
Naresh Kumar
H.V. Raghu
author_sort Soniya A. Ranveer
collection DOAJ
description According to the results of this study, the paper strip biosensor can detect pesticide at very low concentration like fungicide, organochlorine, organophosphate, carbamate, and herbicide group ranges from 1 to 10, 1–50, 250–500, 1–50, and 1 μg/L, respectively in animal feed, water, milk and soil. This is a significant improvement from the previous study, which found that the paper strip biosensor could only detect pesticide levels of up to 500 or 1000 μg/L. A total of 436 samples were collected from the dairy farm, including 58 samples of green feed, 54 samples of dry feed, 45 samples of concentrated feed, 41 samples of fermented feed, 49 samples of manure, 54 samples of soil, and 86 samples of milk. PSA (Primary Secondary Amine) and MgSO4 (1:2 ratio) were used to remove pigments from dairy farm samples to prevent the enzyme–pesticide interaction leading to colour development on the strip, which was successfully achieved. Using a strip-based test and an optimized extraction protocol, pesticides were detected in 38.49% in the samples. Limit of Detection of 15 pesticides from the organochlorine, organophosphate, carbamate, neonicotinoid, pyrethroid, ryanoid, strobilurins, and triazole groups recommended for use in dairy farms were evaluated in feed/fodder. Pesticides were being detected in various dairy farm matrices using the newly developed test. The developed technology can be used as a semi-quantitative test for pesticides monitoring in the dairy farm as well as for screening of primary produce under field condition for organic certification of various food/feed commodities.
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spelling doaj.art-807f6acfc20941fc9175fe006f0f5d562023-06-22T05:04:48ZengElsevierCurrent Research in Food Science2665-92712023-01-016100416Assessment of developed paper strip based sensor with pesticide residues in different dairy environmental samplesSoniya A. Ranveer0C.G. Harshitha1Vaishali Dasriya2Nimisha Tehri3Naresh Kumar4H.V. Raghu5Microbial Biosensors Food Safety Lab, Dairy Microbiology Division, ICAR-National Dairy Research Institute, KArnal, 132001, Haryana, IndiaChemistry Section, National Referral Centre for Milk Quality and Safety, Dairy Chemistry Division, ICAR-National Dairy Research Institute, Karnal, 132001, Haryana, IndiaNational Referral Centre for Milk Quality and Safety, Dairy Microbiology Division, ICAR-National Dairy Research Institute, Karnal, 132001, Haryana, IndiaKothari Postdoc Fellow, Centre for Biotechnology, Maharshi Dayanand University, Rohtak, 124001, Haryana, IndiaMicrobial Biosensors Food Safety Lab, Dairy Microbiology Division, ICAR-National Dairy Research Institute, KArnal, 132001, Haryana, India; National Referral Centre for Milk Quality and Safety, Dairy Microbiology Division, ICAR-National Dairy Research Institute, Karnal, 132001, Haryana, IndiaMicrobial Biosensors Food Safety Lab, Dairy Microbiology Division, ICAR-National Dairy Research Institute, KArnal, 132001, Haryana, India; National Referral Centre for Milk Quality and Safety, Dairy Microbiology Division, ICAR-National Dairy Research Institute, Karnal, 132001, Haryana, India; Corresponding author. National Referral Centre for milk quality and safety, Dairy Microbiology Division, ICAR-National Dairy Research Institute, Karnal, 132001, Haryana, India.According to the results of this study, the paper strip biosensor can detect pesticide at very low concentration like fungicide, organochlorine, organophosphate, carbamate, and herbicide group ranges from 1 to 10, 1–50, 250–500, 1–50, and 1 μg/L, respectively in animal feed, water, milk and soil. This is a significant improvement from the previous study, which found that the paper strip biosensor could only detect pesticide levels of up to 500 or 1000 μg/L. A total of 436 samples were collected from the dairy farm, including 58 samples of green feed, 54 samples of dry feed, 45 samples of concentrated feed, 41 samples of fermented feed, 49 samples of manure, 54 samples of soil, and 86 samples of milk. PSA (Primary Secondary Amine) and MgSO4 (1:2 ratio) were used to remove pigments from dairy farm samples to prevent the enzyme–pesticide interaction leading to colour development on the strip, which was successfully achieved. Using a strip-based test and an optimized extraction protocol, pesticides were detected in 38.49% in the samples. Limit of Detection of 15 pesticides from the organochlorine, organophosphate, carbamate, neonicotinoid, pyrethroid, ryanoid, strobilurins, and triazole groups recommended for use in dairy farms were evaluated in feed/fodder. Pesticides were being detected in various dairy farm matrices using the newly developed test. The developed technology can be used as a semi-quantitative test for pesticides monitoring in the dairy farm as well as for screening of primary produce under field condition for organic certification of various food/feed commodities.http://www.sciencedirect.com/science/article/pii/S2665927122002428Strip based sensorPesticide residuesBiosensorAnimal feedMilkDetection
spellingShingle Soniya A. Ranveer
C.G. Harshitha
Vaishali Dasriya
Nimisha Tehri
Naresh Kumar
H.V. Raghu
Assessment of developed paper strip based sensor with pesticide residues in different dairy environmental samples
Current Research in Food Science
Strip based sensor
Pesticide residues
Biosensor
Animal feed
Milk
Detection
title Assessment of developed paper strip based sensor with pesticide residues in different dairy environmental samples
title_full Assessment of developed paper strip based sensor with pesticide residues in different dairy environmental samples
title_fullStr Assessment of developed paper strip based sensor with pesticide residues in different dairy environmental samples
title_full_unstemmed Assessment of developed paper strip based sensor with pesticide residues in different dairy environmental samples
title_short Assessment of developed paper strip based sensor with pesticide residues in different dairy environmental samples
title_sort assessment of developed paper strip based sensor with pesticide residues in different dairy environmental samples
topic Strip based sensor
Pesticide residues
Biosensor
Animal feed
Milk
Detection
url http://www.sciencedirect.com/science/article/pii/S2665927122002428
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AT nimishatehri assessmentofdevelopedpaperstripbasedsensorwithpesticideresiduesindifferentdairyenvironmentalsamples
AT nareshkumar assessmentofdevelopedpaperstripbasedsensorwithpesticideresiduesindifferentdairyenvironmentalsamples
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