Uptake of <sup>3</sup>H-cAMP by retinal pigment epithelium isolated from bluegill sunfish (<it>Lepomis macrochirus</it>)
<p>Abstract</p> <p>Background</p> <p>In bluegill sunfish, the melanin-containing pigment granules of the retinal pigment epithelium undergo cyclic movements in response both to ambient lighting and circadian cues. Pigment granules aggregate into the cell body at night (...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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BMC
2006-12-01
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| Series: | BMC Neuroscience |
| Online Access: | http://www.biomedcentral.com/1471-2202/7/82 |
| _version_ | 1819027335464091648 |
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| author | Moredock Steve Radhakrishnan Varsha Keith Thomas A García Dana M |
| author_facet | Moredock Steve Radhakrishnan Varsha Keith Thomas A García Dana M |
| author_sort | Moredock Steve |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>In bluegill sunfish, the melanin-containing pigment granules of the retinal pigment epithelium undergo cyclic movements in response both to ambient lighting and circadian cues. Pigment granules aggregate into the cell body at night (in the dark), and disperse into apical processes during the day (in the light). Regulation of pigment granule aggregation in a number of fishes depends on modulating the intracellular levels of cyclic adenosine monophosphate.</p> <p>Results</p> <p>Here we show isolated RPE takes up cyclic adenosine monophosphate (cAMP) in a saturable manner, exogenously applied cAMP induces pigment granule aggregation in retinal pigment epithelium isolated from bluegill, and aggregation induced in this manner is inhibited by treatment with probenecid, an organic anion transport inhibitor.</p> <p>Conclusion</p> <p>Our results raise the possibility that cAMP functions as a messenger secreted from the neural retina to signal darkness to the RPE, which takes it up. It further suggests that organic anion transport systems are the route by which cAMP crosses RPE cell membranes since probenecid inhibits extracellular cAMP from causing pigment granule aggregation.</p> |
| first_indexed | 2024-12-21T05:40:50Z |
| format | Article |
| id | doaj.art-8084d53be3df4444a9edba08a1ed7a49 |
| institution | Directory Open Access Journal |
| issn | 1471-2202 |
| language | English |
| last_indexed | 2024-12-21T05:40:50Z |
| publishDate | 2006-12-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Neuroscience |
| spelling | doaj.art-8084d53be3df4444a9edba08a1ed7a492022-12-21T19:14:16ZengBMCBMC Neuroscience1471-22022006-12-01718210.1186/1471-2202-7-82Uptake of <sup>3</sup>H-cAMP by retinal pigment epithelium isolated from bluegill sunfish (<it>Lepomis macrochirus</it>)Moredock SteveRadhakrishnan VarshaKeith Thomas AGarcía Dana M<p>Abstract</p> <p>Background</p> <p>In bluegill sunfish, the melanin-containing pigment granules of the retinal pigment epithelium undergo cyclic movements in response both to ambient lighting and circadian cues. Pigment granules aggregate into the cell body at night (in the dark), and disperse into apical processes during the day (in the light). Regulation of pigment granule aggregation in a number of fishes depends on modulating the intracellular levels of cyclic adenosine monophosphate.</p> <p>Results</p> <p>Here we show isolated RPE takes up cyclic adenosine monophosphate (cAMP) in a saturable manner, exogenously applied cAMP induces pigment granule aggregation in retinal pigment epithelium isolated from bluegill, and aggregation induced in this manner is inhibited by treatment with probenecid, an organic anion transport inhibitor.</p> <p>Conclusion</p> <p>Our results raise the possibility that cAMP functions as a messenger secreted from the neural retina to signal darkness to the RPE, which takes it up. It further suggests that organic anion transport systems are the route by which cAMP crosses RPE cell membranes since probenecid inhibits extracellular cAMP from causing pigment granule aggregation.</p>http://www.biomedcentral.com/1471-2202/7/82 |
| spellingShingle | Moredock Steve Radhakrishnan Varsha Keith Thomas A García Dana M Uptake of <sup>3</sup>H-cAMP by retinal pigment epithelium isolated from bluegill sunfish (<it>Lepomis macrochirus</it>) BMC Neuroscience |
| title | Uptake of <sup>3</sup>H-cAMP by retinal pigment epithelium isolated from bluegill sunfish (<it>Lepomis macrochirus</it>) |
| title_full | Uptake of <sup>3</sup>H-cAMP by retinal pigment epithelium isolated from bluegill sunfish (<it>Lepomis macrochirus</it>) |
| title_fullStr | Uptake of <sup>3</sup>H-cAMP by retinal pigment epithelium isolated from bluegill sunfish (<it>Lepomis macrochirus</it>) |
| title_full_unstemmed | Uptake of <sup>3</sup>H-cAMP by retinal pigment epithelium isolated from bluegill sunfish (<it>Lepomis macrochirus</it>) |
| title_short | Uptake of <sup>3</sup>H-cAMP by retinal pigment epithelium isolated from bluegill sunfish (<it>Lepomis macrochirus</it>) |
| title_sort | uptake of sup 3 sup h camp by retinal pigment epithelium isolated from bluegill sunfish it lepomis macrochirus it |
| url | http://www.biomedcentral.com/1471-2202/7/82 |
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