Phenotype and function of MAIT cells in patients with alveolar echinococcosis
Mucosal-associated invariant T (MAIT) cells are a subpopulation of unconventional T cells widely involved in chronic liver diseases. However, the potential role and regulating factors of MAIT cells in alveolar echinococcosis (AE), a zoonotic parasitic disease by Echinococcus multilocularis (E. multi...
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Frontiers Media S.A.
2024-03-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2024.1343567/full |
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| author | Jintian Li Jintian Li Hanyue Zhao Hanyue Zhao Guodong Lv Kalibixiati Aimulajiang Liang Li Renyong Lin Renyong Lin Tuerganaili Aji Tuerganaili Aji Tuerganaili Aji |
| author_facet | Jintian Li Jintian Li Hanyue Zhao Hanyue Zhao Guodong Lv Kalibixiati Aimulajiang Liang Li Renyong Lin Renyong Lin Tuerganaili Aji Tuerganaili Aji Tuerganaili Aji |
| author_sort | Jintian Li |
| collection | DOAJ |
| description | Mucosal-associated invariant T (MAIT) cells are a subpopulation of unconventional T cells widely involved in chronic liver diseases. However, the potential role and regulating factors of MAIT cells in alveolar echinococcosis (AE), a zoonotic parasitic disease by Echinococcus multilocularis (E. multilocularis) larvae chronically parasitizing liver organs, has not yet been studied. Blood samples (n=29) and liver specimens (n=10) from AE patients were enrolled. The frequency, phenotype, and function of MAIT cells in peripheral blood and liver tissues of AE patients were detected by flow cytometry. The morphology and fibrosis of liver tissue were examined by histopathology and immunohistochemistry. The correlation between peripheral MAIT cell frequency and serologic markers was assessed by collecting clinicopathologic characteristics of AE patients. And the effect of in vitro stimulation with E. multilocularis antigen (Emp) on MAIT cells. In this study, MAIT cells are decreased in peripheral blood and increased in the close-to-lesion liver tissues, especially in areas of fibrosis. Circulating MAIT exhibited activation and exhaustion phenotypes, and intrahepatic MAIT cells showed increased activation phenotypes with increased IFN-γ and IL-17A, and high expression of CXCR5 chemokine receptor. Furthermore, the frequency of circulating MAIT cells was correlated with the size of the lesions and liver function in patients with AE. After excision of the lesion site, circulating MAIT cells returned to normal levels, and the serum cytokines IL-8, IL-12, and IL-18, associated with MAIT cell activation and apoptosis, were altered. Our results demonstrate the status of MAIT cell distribution, functional phenotype, and migration in peripheral blood and tissues of AE patients, highlighting their potential as biomarkers and therapeutic targets. |
| first_indexed | 2024-04-25T00:07:05Z |
| format | Article |
| id | doaj.art-808505442cd64d67b3a524076b248457 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-04-25T00:07:05Z |
| publishDate | 2024-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-808505442cd64d67b3a524076b2484572024-03-14T05:07:53ZengFrontiers Media S.A.Frontiers in Immunology1664-32242024-03-011510.3389/fimmu.2024.13435671343567Phenotype and function of MAIT cells in patients with alveolar echinococcosisJintian Li0Jintian Li1Hanyue Zhao2Hanyue Zhao3Guodong Lv4Kalibixiati Aimulajiang5Liang Li6Renyong Lin7Renyong Lin8Tuerganaili Aji9Tuerganaili Aji10Tuerganaili Aji11School of Public Healthy, Xinjiang Medical University, Urumqi, ChinaState Key Laboratory of Pathogenesis, Prevention, and Treatment of Central Asian High Incidence Diseases, Clinical Medical Research Institute, First Affiliated Hospital of Xinjiang Medical University, Urumqi, ChinaState Key Laboratory of Pathogenesis, Prevention, and Treatment of Central Asian High Incidence Diseases, Clinical Medical Research Institute, First Affiliated Hospital of Xinjiang Medical University, Urumqi, ChinaDepartment of Hepatobiliary & Hydatid Disease, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, ChinaState Key Laboratory of Pathogenesis, Prevention, and Treatment of Central Asian High Incidence Diseases, Clinical Medical Research Institute, First Affiliated Hospital of Xinjiang Medical University, Urumqi, ChinaState Key Laboratory of Pathogenesis, Prevention, and Treatment of Central Asian High Incidence Diseases, Clinical Medical Research Institute, First Affiliated Hospital of Xinjiang Medical University, Urumqi, ChinaState Key Laboratory of Pathogenesis, Prevention, and Treatment of Central Asian High Incidence Diseases, Clinical Medical Research Institute, First Affiliated Hospital of Xinjiang Medical University, Urumqi, ChinaState Key Laboratory of Pathogenesis, Prevention, and Treatment of Central Asian High Incidence Diseases, Clinical Medical Research Institute, First Affiliated Hospital of Xinjiang Medical University, Urumqi, ChinaDepartment of Hepatobiliary & Hydatid Disease, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, ChinaSchool of Public Healthy, Xinjiang Medical University, Urumqi, ChinaState Key Laboratory of Pathogenesis, Prevention, and Treatment of Central Asian High Incidence Diseases, Clinical Medical Research Institute, First Affiliated Hospital of Xinjiang Medical University, Urumqi, ChinaDepartment of Hepatobiliary & Hydatid Disease, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, ChinaMucosal-associated invariant T (MAIT) cells are a subpopulation of unconventional T cells widely involved in chronic liver diseases. However, the potential role and regulating factors of MAIT cells in alveolar echinococcosis (AE), a zoonotic parasitic disease by Echinococcus multilocularis (E. multilocularis) larvae chronically parasitizing liver organs, has not yet been studied. Blood samples (n=29) and liver specimens (n=10) from AE patients were enrolled. The frequency, phenotype, and function of MAIT cells in peripheral blood and liver tissues of AE patients were detected by flow cytometry. The morphology and fibrosis of liver tissue were examined by histopathology and immunohistochemistry. The correlation between peripheral MAIT cell frequency and serologic markers was assessed by collecting clinicopathologic characteristics of AE patients. And the effect of in vitro stimulation with E. multilocularis antigen (Emp) on MAIT cells. In this study, MAIT cells are decreased in peripheral blood and increased in the close-to-lesion liver tissues, especially in areas of fibrosis. Circulating MAIT exhibited activation and exhaustion phenotypes, and intrahepatic MAIT cells showed increased activation phenotypes with increased IFN-γ and IL-17A, and high expression of CXCR5 chemokine receptor. Furthermore, the frequency of circulating MAIT cells was correlated with the size of the lesions and liver function in patients with AE. After excision of the lesion site, circulating MAIT cells returned to normal levels, and the serum cytokines IL-8, IL-12, and IL-18, associated with MAIT cell activation and apoptosis, were altered. Our results demonstrate the status of MAIT cell distribution, functional phenotype, and migration in peripheral blood and tissues of AE patients, highlighting their potential as biomarkers and therapeutic targets.https://www.frontiersin.org/articles/10.3389/fimmu.2024.1343567/fullmucosa-associated invariant T cellsalveolar echinococcosisparasitic lesionpro-fibrogenicserum cytokine |
| spellingShingle | Jintian Li Jintian Li Hanyue Zhao Hanyue Zhao Guodong Lv Kalibixiati Aimulajiang Liang Li Renyong Lin Renyong Lin Tuerganaili Aji Tuerganaili Aji Tuerganaili Aji Phenotype and function of MAIT cells in patients with alveolar echinococcosis Frontiers in Immunology mucosa-associated invariant T cells alveolar echinococcosis parasitic lesion pro-fibrogenic serum cytokine |
| title | Phenotype and function of MAIT cells in patients with alveolar echinococcosis |
| title_full | Phenotype and function of MAIT cells in patients with alveolar echinococcosis |
| title_fullStr | Phenotype and function of MAIT cells in patients with alveolar echinococcosis |
| title_full_unstemmed | Phenotype and function of MAIT cells in patients with alveolar echinococcosis |
| title_short | Phenotype and function of MAIT cells in patients with alveolar echinococcosis |
| title_sort | phenotype and function of mait cells in patients with alveolar echinococcosis |
| topic | mucosa-associated invariant T cells alveolar echinococcosis parasitic lesion pro-fibrogenic serum cytokine |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2024.1343567/full |
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